Manipulation of droplets on hydrophilic or variegated-hydrophilic surfaces

Patent metadata

Field	Value
Publication number	US-2016199832-A1
Application number	US-201414912394-A
Country	US
Kind code	A1
Filing date	Aug 29, 2014
Priority date	Aug 30, 2013
Publication date	Jul 14, 2016
Grant date	—

Abstract

Provided herein is a droplet actuator including (a) first and second substrates separated by a droplet-operations gap, the first and second substrates including respective hydrophobic surfaces that face the droplet-operations gap; (b) a plurality of electrodes coupled to at least one of the first substrate and the second substrate, the electrodes arranged along the droplet-operations gap to control movement of a droplet along the hydrophobic surfaces within the droplet-operations gap; and (c) a hydrophilic or variegated-hydrophilic surface exposed to the droplet-operations gap, the hydrophilic or variegated-hydrophilic surface being positioned to contact the droplet when the droplet is at a select position within the droplet-operations gap.

First claim

1 . A droplet actuator comprising:
first and second substrates separated by a droplet-operations gap, the first and second substrates including respective hydrophobic surfaces that face the droplet-operations gap; a plurality of electrodes coupled to at least one of the first substrate and the second substrate, the electrodes arranged along the droplet-operations gap to control movement of a droplet along the hydrophobic surfaces within the droplet-operations gap; and a hydrophilic or variegated-hydrophilic surface exposed to the droplet-operations gap, the hydrophilic or variegated-hydrophilic surface being positioned to contact the droplet when the droplet is at a select position within the droplet-operations gap.

2 . The droplet actuator of claim 1 , wherein the hydrophobic surfaces include at least one of a tetrafluoroethylene polymer, a fluoropolymer, and an amorphous fluoropolymer.

3 . The droplet actuator of claim 1 , wherein the variegated-hydrophilic surface comprises a rough surface that forms interstitial regions that separate a plurality of nanowells.

4 .- 7 . (canceled)

8 . The droplet actuator of claim 1 , wherein the electrodes are positioned to transport the droplet toward the hydrophilic or variegated-hydrophilic surface, or wherein the electrodes are positioned to transport the droplet away from the hydrophilic or variegated-hydrophilic surface.

9 . (canceled)

10 . The droplet actuator of claim 1 , further comprising a controller, the controller configured to control the electrodes to transport the droplet onto the hydrophilic or variegated-hydrophilic surface from at least one of the hydrophobic surfaces, or configured to control the electrodes to transport the droplet onto at least one of the hydrophobic surfaces from the hydrophilic or variegated-hydrophilic surface.

11 .- 15 . (canceled)

16 . The droplet actuator of claim 1 , wherein the droplet is aligned with a designated electrode when at the select position, such that the designated electrode faces and is adjacent to the droplet within the droplet-operations gap.

17 . The droplet actuator of claim 16 , wherein the hydrophilic or variegated-hydrophilic surface is positioned to face the designated electrode with the droplet-operations gap therebetween.

18 . (canceled)

19 . The droplet actuator of claim 16 , wherein the hydrophilic or variegated-hydrophilic surface is arranged between the first and second substrates.

20 .- 25 . (canceled)

26 . The droplet actuator of claim 16 , wherein the droplet-operations gap has a gap height, the gap height at the designated electrode being different than the gap height at an electrode adjacent to the designated electrode such that the droplet has a different height when aligned with the designated electrode than when aligned with the adjacent electrode.

27 .- 36 . (canceled)

37 . The droplet actuator of claim 1 , wherein the variegated-hydrophilic surface has hydrophilic portions and superhydrophobic portions within the footprint.

38 .- 49 . (canceled)

50 . The droplet actuator of claim 1 , wherein a dielectric layer is positioned between the hydrophilic or variegated-hydrophilic surface and the electrodes.

51 .- 56 . (canceled)

57 . The droplet actuator of claim 1 , further comprising an optical detector coupled to one of the first substrate or the second substrate, the hydrophilic or variegated-hydrophilic surface being aligned with the optical detector for detecting light signals from the hydrophilic surface.

58 . A method comprising:
providing a droplet actuator including a droplet-operations gap and a plurality of electrodes positioned along the droplet-operations gap, the droplet-operations gap being defined between opposing hydrophobic surfaces, the droplet actuator having a hydrophilic or variegated-hydrophilic surface exposed to the droplet-operations gap; controlling the electrodes to transport a droplet using electrowetting-mediated droplet operations through the droplet-operations gap along the hydrophobic surfaces to a select position, wherein the droplet is in contact with the hydrophilic or variegated-hydrophilic surface when the droplet in a select position.

59 .- 64 . (canceled)

65 . The method of claim 58 , wherein controlling the electrodes to transport the droplet includes transporting the droplet toward the hydrophilic or variegated-hydrophilic surface, or wherein controlling the electrodes to transport the droplet includes transporting the droplet away from the hydrophilic or variegated-hydrophilic surface.

66 .- 70 . (canceled)

71 . The method of claim 58 , wherein the droplet is a first droplet, the method further comprising controlling the electrodes to move a second droplet to engage the first droplet and displace the first droplet from the select position.

72 . The method of claim 71 , further comprising controlling the electrodes to move the first droplet further away from the select position after the first droplet has been displaced.

73 .- 76 . (canceled)

77 . The method of claim 58 , wherein the droplet is a first droplet, the method further comprising controlling a second droplet to engage and combine with the first droplet at the select position and form a combined droplet, the method further comprising moving at least a portion of the combined droplet away from the select position.

78 . The method of claim 77 , wherein the first droplet has a volume such that the first droplet aligns with multiple electrodes when in the select position, the second droplet having a volume that is smaller than the first droplet, wherein the portion of the combined droplet that is moved away from the select position is substantially equal to a volume of the second droplet.

79 . The method of claim 58 , wherein
the droplet is a first droplet, the method further comprising moving a second droplet toward the first droplet with a filler fluid therebetween thereby generating a pumping force, the pumping force displacing the first droplet from the select position.

80 . (canceled)

81 . The method of claim 79 , wherein the second droplet has a reservoir volume, the method further comprising splitting the second droplet to form the first droplet and then moving the first droplet through the pumping force.

82 .- 113 . (canceled)

Assignees

• Illumina Inc
• Advanced Liquid Logic France Sas

Inventors

• Jamshidi Arash
• Lin Yan-You
• Aravanis Alex
• Delattre Cyril
• Rival Arnaud
• Foley Jennifer
• Sabounchi Poorya
• Khurana Tarun
• Babazadeh Majid
• Amini Hamed
• Venkatesan Bala Murali
• Bowen M Shane
• Barnard Steven M
• Rogert Bacigalupo Maria Candelaria
• Dehlinger Dietrich

Classifications

• B01L2300/166

  Suprahydrophobic; Ultraphobic; Lotus-effect · CPC title

• B01L2400/0427

  Electrowetting · CPC title

• B01L2300/161

  Control and use of surface tension forces, e.g. hydrophobic, hydrophilic · CPC title

• B01L3/502792

  for moving individual droplets on a plate, e.g. by locally altering surface tension · CPC title

• B01L2300/0819

  Microarrays; Biochips · CPC title