Phytase

Patent metadata

Field	Value
Publication number	US-2016194618-A1
Application number	US-201414904840-A
Country	US
Kind code	A1
Filing date	Mar 10, 2014
Priority date	Jul 25, 2013
Publication date	Jul 7, 2016
Grant date	—

Abstract

Polypeptides having phytase activity and polynucleotide sequences encoding the phytases are provided. The gene expresses the phytase at a level of at least 7 g/l to 40 g/L. The phytases have higher specific activity, retain activity at low pH, retain activity at high temperature, increased phosphorous equivalency, increased phosphorous bioavailability, and increased phosphorous hydrolysis. The phytases can be used in a variety of industries including food, feed, ethanol production, pharmaceuticals, and cleaning.

First claim

1 . A phytase having increased phosphorous equivalency when compared to a fungal, E. coli , or a modified E. coli phytase.

2 . The phytase of claim 1 , wherein the phytase has a phosphorous equivalency in a range from 0.11 to 0.23%.

3 . A phytase having increased phosphorous bioavailability when compared to a fungal, E. coli , or modified E. coli phytase.

4 . The phytase of claim 3 , wherein the phytase has at least 60 to 65% more phosphorous bioavailability when compared to a fungal or E. coli phytase.

5 . The phytase of claim 3 , wherein the phytase has least 20 to 30% more phosphorous bioavailability when compared to a modified E. coli phytase.

6 . A phytase capable of increased phosphorous hydrolysis when compared to a fungal, E. coli , or modified E. coli phytase.

7 . The phytase of claim 6 , wherein the phytase hydrolyzes up to 90% of phosphorous from a feed comprising phytic acid.

8 . A phytase capable of hydrolyzing up to 90% of phosphorous from a feed comprising phytic acid.

9 . A phytase having at least 100% activity for more than 5 minutes at low pH.

10 . A phytase having improved activity at phytic acid concentrations from 0.2 mM to 5.1 mM of phytic acid.

11 . A phytase having a melting temperature greater than about 90 degrees C.

12 . A phytase having at least 90% activity at 2.5 minutes.

13 . A phytase that hydrolyzes anti-nutritive phytase IP2, IP3, IP4, IP5, and IP6 from a range of about 91% to about 98%.

Assignees

• Basf Enzymes Llc

Inventors

• Solbak Arne I

Classifications

• C12N9/16 Primary

  acting on ester bonds (3.1) · CPC title

• C12Y301/03008

  3-Phytase (3.1.3.8) · CPC title

• Y02E50/10

  Biofuels, e.g. bio-diesel · CPC title

• A23K50/75

  for poultry · CPC title

• A23K20/189

  Enzymes · CPC title