Mesostructured polymer membranes and other articles

Patent metadata

Field	Value
Publication number	US-2016193385-A1
Application number	US-201414911792-A
Country	US
Kind code	A1
Filing date	Aug 14, 2014
Priority date	Aug 16, 2013
Publication date	Jul 7, 2016
Grant date	—

Abstract

The present invention generally relates to porous membranes and other porous articles. In one aspect, the present invention is generally directed to porous membranes and other articles that have a pore size comparable to feature sizes of the extracellular matrix. Such articles may be useful, for example, for tissue engineering (e.g., as a substrate for culturing cells), as a filter, or for other applications. In some cases, the membranes may be formed from biocompatible and/or biodegradable materials. In some embodiments, such membranes may be formed using solvent evaporation induced self-assembly (EISA) techniques, although other techniques may be used in other embodiments. Still other aspects of the present invention are directed to methods of using such articles, kits involving such articles, and the like.

First claim

What is claimed is:

1 . A composition, comprising:
a porous article comprising an amphiphilic block copolymer and a hydrophobic block copolymer, the porous article comprising pores having an average pore size of between about 100 nm and about 1 micrometer, as determined using SEM.

2 . The composition of claim 1 , wherein the pores of the porous article has an average pore aspect ratio of at least about 2.

3 . The composition of any one of claim 1 or 2 , wherein the pores of the porous article has an average pore aspect ratio of at least about 3.

4 . The composition of any one of claims 1 - 3 , wherein the pores of the porous article has an average pore aspect ratio of at least about 4.

5 . The composition of any one of claims 1 - 4 , wherein the article has a mass ratio of the hydrophobic block copolymer to the amphiphilic block copolymer of between about 1:1 and about 1:10.

6 . The composition of any one of claims 1 - 5 , wherein the article has a mass ratio of the hydrophobic block copolymer to the amphiphilic block copolymer of between about 1:2 and about 1:8.

7 . The composition of any one of claims 1 - 6 , wherein the article comprises fibers.

8 . The composition of claim 7 , wherein at least about 80 wt % of the article comprises fibers.

9 . The composition of any one of claim 7 or 8 , wherein the fibers have an average diameter of between about 50 nm and about 500 nm.

10 . The composition of any one of claims 7 - 9 , wherein the fibers have an average diameter of between about 100 nm and about 200 nm.

11 . The composition of any one of claims 1 - 10 , wherein at least about 25 wt % of the article comprises the amphiphilic block copolymer and the hydrophobic block copolymer.

12 . The composition of any one of claims 1 - 11 , wherein at least about 50 wt % of the article comprises the amphiphilic block copolymer and the hydrophobic block copolymer.

13 . The composition of any one of claims 1 - 12 , wherein at least about 90 wt % of the article comprises the amphiphilic block copolymer and the hydrophobic block copolymer.

14 . The composition of any one of claims 1 - 13 , wherein at least about 95 wt % of the article comprises the amphiphilic block copolymer and the hydrophobic block copolymer.

15 . The composition of any one of claims 1 - 14 , wherein the hydrophobic block copolymer comprises a polyester.

16 . The composition of claim 15 , wherein at least some of the polyester comprises polylactide.

17 . The composition of any one of claim 15 or 16 , wherein at least some of the polyester comprises polyglycolide.

18 . The composition of any one of claims 15 - 17 , wherein at least some of the polyester comprises poly(lactide-co-glycolide).

19 . The composition of claim 18 , wherein the poly(lactide-co-glycolide) comprises a ratio of between about 1:100 and about 100:1 of lactide to glycolide by mass.

20 . The composition of any one of claim 18 or 19 , wherein the poly(lactide-co-glycolide) comprises a ratio of between about 1:30 and about 30:1 of lactide to glycolide by mass.

21 . The composition of any one of claims 18 - 20 , wherein the poly(lactide-co-glycolide) comprises a ratio of between about 1:5 and about 5:1 of lactide to glycolide by mass.

22 . The composition of any one of claims 18 - 21 , wherein the poly(lactide-co-glycolide) comprises a ratio of between about 1:2 and about 2:1 of lactide to glycolide by mass.

23 . The composition of any one of claims 1 - 22 , wherein at least some of the amphiphilic block copolymer comprises a polyol.

24 . The composition of claim 23 , wherein at least some of the polyol comprises poly(ethylene glycol).

25 . The composition of any one of claim 23 or 24 , wherein at least some of the polyol comprises poly(propylene glycol).

26 . The composition of any one of claims 23 - 25 , wherein at least some of the polyol comprises a copolymer comprising poly(ethylene glycol) and poly(propylene glycol).

27 . The composition of any one of claims 23 - 26 , wherein at least some of the polyol comprises triblock poly(ethylene glycol)-poly(propylene glycol)-poly(ethylene glycol).

28 . The composition of any one of claims 1 - 27 , wherein the average pore size is between about 200 nm and about 500 nm.

29 . The composition of any one of claims 1 - 28 , wherein the average pore size is between about 300 nm and about 400 nm.

30 . The composition of any one of claims 1 - 29 , wherein the pores are arranged as pore domains having an average dimension of between about 10 micrometers and about 1000 micrometers, as determined using SEM, the pores being substantially concentrically arranged within the pore domain.

31 . The composition of claim 30 , wherein the pore domains have an average dimension of between about 15 micrometers and about 500 micrometers.

32 . The composition of any one of claim 30 or 31 , wherein the pore domains have an average dimension of between about 20 micrometers and about 200 micrometers.

33 . The composition of any one of claims 1 - 32 , wherein at least some of the hydrophobic block copolymer is biocompatible.

34 . The composition of any one of claims 1 - 33 , wherein at least some of the hydrophobic block copolymer is biodegradable.

35 . The composition of any one of claims 1 - 34 , wherein at least some of the amphiphilic block copolymer is biocompatible.

36 . The composition of any one of claims 1 - 35 , wherein at least some of the amphiphilic block copolymer is biodegradable.

37 . The composition of any one of claims 1 - 36 , wherein the article is a membrane.

38 . The composition of any one of claims 1 - 37 , wherein the article is in physical contact with cell culture media.

39 . The composition of any one of claims 1 - 38 , wherein the article comprises mammalian cells.

40 . The composition of any one of claims 1 - 39 , wherein the article is implanted within a mammal.

41 . The composition of any one of claims 1 - 40 , wherein the article is a coating on a substrate.

42 . The composition of any one of claims 1 - 41 , wherein the article is implantable.

43 . The composition of any one of claims 1 - 42 , wherein the article further comprises a peptide.

44 . The composition of claim 43 , wherein the peptide comprises cyclic RGD peptide.

45 . The composition of any one of claims 1 - 44 , wherein the article has an average tensile modulus of between about 1 MPa and about 100 MPa.

46 . The composition of any one of claims 1 - 45 , wherein the article has an average tensile modulus of between about 10 MPa and about 50 MPa.

47 . The composition of any one of claims 1 - 46 , wherein the article has a contact angle of less than about 30°.

48 . The composition of any one of claims 1 - 47 , wherein the article has a contact angle of less than about 20°.

49 . The composition of any one of claims 1 - 48 , wherein the article is substantially nonionic.

50 . A method,

Assignees

• Childrens Medical Center

Inventors

• Tian Bozhi
• Kohane Daniel S

Classifications

• A61L27/56 Primary

  Porous materials, {e.g. foams or sponges} · CPC title

• C08J9/26

  by elimination of a solid phase from a macromolecular composition or article, e.g. leaching out · CPC title

• C08J2205/044

  Micropores, i.e. average diameter being between 0,1 micrometer and 0,1 millimeter · CPC title

• C08J9/0085

  Use of fibrous compounding ingredients (C08J9/0076 takes precedence) · CPC title

• C08J2467/04

  Polyesters derived from hydroxy carboxylic acids, e.g. lactones (C08J2467/06 takes precedence) · CPC title