Gho/sec24b2 and sec24b1 nucleic acid molecules to control coleopteran and hemipteran pests

Patent metadata

Field	Value
Publication number	US-2016186203-A1
Application number	US-201514878852-A
Country	US
Kind code	A1
Filing date	Oct 8, 2015
Priority date	Oct 8, 2014
Publication date	Jun 30, 2016
Grant date	—

Abstract

This disclosure concerns nucleic acid molecules and methods of use thereof for control of insect pests through RNA interference-mediated inhibition of target coding and transcribed non-coding sequences in insect pests, including coleopteran and/or hemipteran pests. The disclosure also concerns methods for making transgenic plants that express nucleic acid molecules useful for the control of insect pests, and the plant cells and plants obtained thereby.

First claim

What may be claimed is:

1 . An isolated nucleic acid comprising at least one polynucleotide operably linked to a heterologous promoter, wherein the polynucleotide is selected from the group consisting of:
SEQ ID NO:1; the complement of SEQ ID NO:1; a fragment of at least 15 contiguous nucleotides of SEQ ID NO:1; the complement of a fragment of at least 15 contiguous nucleotides of SEQ ID NO:1; a native coding sequence of a Diabrotica organism comprising SEQ ID NO:1; the complement of a native coding sequence of a Diabrotica organism comprising SEQ ID NO:1; a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Diabrotica organism comprising SEQ ID NO:1; the complement of a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Diabrotica organism comprising SEQ ID NO:1; SEQ ID NO:102; the complement of SEQ ID NO:102; a fragment of at least 15 contiguous nucleotides of SEQ ID NO:102; the complement of a fragment of at least 15 contiguous nucleotides of SEQ ID NO:102; a native coding sequence of a Diabrotica organism comprising SEQ ID NO:102; the complement of a native coding sequence of a Diabrotica organism comprising SEQ ID NO:102; a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Diabrotica organism comprising SEQ ID NO:102; the complement of a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Diabrotica organism comprising SEQ ID NO:102; SEQ ID NO:107; the complement of SEQ ID NO:107; a fragment of at least 15 contiguous nucleotides of SEQ ID NO:107; the complement of a fragment of at least 15 contiguous nucleotides of SEQ ID NO:107; a native coding sequence of a Diabrotica organism comprising SEQ ID NO:107; the complement of a native coding sequence of a Diabrotica organism comprising SEQ ID NO:107; a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Diabrotica organism comprising SEQ ID NO:107; the complement of a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Diabrotica organism comprising SEQ ID NO:107; SEQ ID NO:84; the complement of SEQ ID NO:84; a fragment of at least 15 contiguous nucleotides of SEQ ID NO:84; the complement of a fragment of at least 15 contiguous nucleotides of SEQ ID NO:84; a native coding sequence of a Euschistus organism comprising SEQ ID NO:84; the complement of a native coding sequence of a Euschistus organism comprising SEQ ID NO:84; a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Euschistus organism comprising SEQ ID NO:84; the complement of a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Euschistus organism comprising SEQ ID NO:84; SEQ ID NO:85; the complement of SEQ ID NO:85; a fragment of at least 15 contiguous nucleotides of SEQ ID NO:85; the complement of a fragment of at least 15 contiguous nucleotides of SEQ ID NO:85; a native coding sequence of a Euschistus organism comprising SEQ ID NO:85; the complement of a native coding sequence of a Euschistus organism comprising SEQ ID NO:85; a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Euschistus organism comprising SEQ ID NO:85; and the complement of a fragment of at least 15 contiguous nucleotides of a native coding sequence of a Euschistus organism comprising SEQ ID NO:85.

2 . The polynucleotide of claim 1 , wherein the polynucleotide is selected from the group consisting of SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:84, SEQ ID NO:85, SEQ ID NO:86, SEQ ID NO:87, SEQ ID NO:88, SEQ ID NO:102, SEQ ID NO:104, SEQ ID NO:107, SEQ ID NO:109, and the complements of any of the foregoing.

3 . A plant transformation vector comprising the polynucleotide of claim 1 .

4 . The polynucleotide of claim 1 , wherein the organism is selected from the group consisting of D. v. virgifera LeConte; D. barberi Smith and Lawrence; D. u. howardi; D. v. zeae; D. balteata LeConte; D. u. tenella; D. u. undecimpunctata Mannerheim; Euschistus heros (Fabr.) (Neotropical Brown Stink Bug), Nezara viridula (L.) (Southern Green Stink Bug), Piezodorus guildinii (Westwood) (Red-banded Stink Bug), Halyomorpha halys (Stål) (Brown Marmorated Stink Bug), Chinavia hilare (Say) (Green Stink Bug), Euschistus servus (Say) (Brown Stink Bug), Dichelops melacanthus (Dallas), Dichelops furcatus (F.), Edessa meditabunda (F.), Thyanta perditor (F.) (Neotropical Red Shouldered Stink Bug), Chinavia marginatum (Palisot de Beauvois), Horcias nobilellus (Berg) (Cotton Bug), Taedia stigmosa (Berg), Dysdercus peruvianus (Guérin-Méneville), Neomegalotomus parvus (Westwood), Leptoglossus zonatus (Dallas), Niesthrea sidae (F.), Lygus hesperus (Knight) (Western Tarnished Plant Bug), and Lygus lineolaris (Palisot de Beauvois).

5 . A ribonucleic acid (RNA) molecule transcribed from the polynucleotide of claim 1 .

6 . A double-stranded ribonucleic acid molecule produced from the expression of the polynucleotide of claim 1 .

7 . The double-stranded ribonucleic acid molecule of claim 6 , wherein contacting the polynucleotide sequence with a coleopteran or hemipteran pest inhibits the expression of an endogenous nucleotide sequence specifically complementary to the polynucleotide.

8 . The double-stranded ribonucleic acid molecule of claim 7 , wherein contacting said ribonucleotide molecule with a coleopteran or hemipteran pest kills or inhibits the growth, and/or feeding of the pest.

9 . The double stranded RNA of claim 6 , comprising a first, a second and a third RNA segment, wherein the first RNA segment comprises the polynucleotide, wherein the third RNA segment is linked to the first RNA segment by the second polynucleotide sequence, and wherein the third RNA segment is substantially the reverse complement of the first RNA segment, such that the first and the third RNA segments hybridize when transcribed into a ribonucleic acid to form the double-stranded RNA.

10 . The RNA of claim 5 , selected from the group consisting of a double-stranded ribonucleic acid molecule and a single-stranded ribonucleic acid molecule of between about 15 and about 30 nucleotides in length.

11 . A plant transformation vector comprising the polynucleotide of claim 1 , wherein the heterologous promoter is functional in a plant cell.

12 . A cell transformed with the polynucleotide of claim 1 .

13 . The cell of claim 12 , wherein the cell is a prokaryotic cell.

14 . The cell of claim 12 , wherein the cell is a eukaryotic cell.

15 . The cell of claim 14 , wherein the cell is a plant cell.

16 . A plant transformed with the polynucleotide of claim 1 .

17 . A seed of the plant of claim 16 , wherein the seed comprises the polynucleotide.

18 . A commodity product produced from the plant of claim 16 , wherein the commodity product comprises a detectable amount of the polynucleotide.

19 . The plant of claim 16 , wherein the at least one polynucleotide is expressed in the plant as a double-stranded ribonucleic acid molecule.

20 . The cell of claim 15 , wherein the cell is a maize, soybean, or cotton cell.

21 . The plant of claim 16 , wherein the plant is maize, soybean, or cotton.

22 . The plant of claim 16 , wherein the at least one polynucleotide is expressed in the plant as a ribonucleic acid molecule, and the ribonucleic acid molecule inhi

Assignees

• Dow Agrosciences Llc
• Univ Nebraska

Inventors

• Narva Kenneth E
• Arora Kanika
• Worden Sarah
• Rangasamy Murugesan
• Li Huarong
• Frey Meghan
• Siegfried Blair
• Khajuria Chitvan
• Fishilevich Elane

Classifications

• C12N15/8286 Primary

  for insect resistance · CPC title

• C12N15/8218

  Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS] · CPC title

• C12N2310/14

  interfering nucleic acids [NA] · CPC title

• C12N2330/51

  Specially adapted vectors · CPC title

• C07K14/325

  Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins · CPC title