Probe and method for detecting metal ions and chemical/biochemical molecules

Patent metadata

Field	Value
Publication number	US-2016178596-A1
Application number	US-201414582844-A
Country	US
Kind code	A1
Filing date	Dec 24, 2014
Priority date	Dec 22, 2014
Publication date	Jun 23, 2016
Grant date	—

Abstract

A method for detecting metal ions and chemical/biochemical molecules is provided. The method includes providing a probe, wherein the probe includes: a gold nanocluster; a reducing agent and a chelating agent partially capped on a surface of the gold nanocluster, wherein the probe is formed of reducing gold ions by the reducing agent, and the gold ions and the reducing agent have a molar ratio of 1:0.7 to 1:1.9. The probe may interact with several metal ions of an aqueous solution to produce different changes of fluorescent spectra. Chemical/biochemical molecules can be detected by the fluorescent spectra difference caused by the interaction between the metal ions and the chemical/biochemical molecules.

First claim

What is claimed is:

1 . A probe, comprising:
a gold nanocluster; and a reducing agent partially capped on a surface of the gold nanocluster; wherein the probe is formed of reducing gold ions by the reducing agent, and the gold ions and the reducing agent have a molar ratio of 1:0.7 to 1:1.9.

2 . The probe as claimed in claim 1 , wherein the reducing agent comprises glutathione, and the probe is used to detect Ag + , Gd 3+ , Zr 4+ , Fe 3+ , Ga 3+ , Pb 2+ , Eu 3+ , or Cu 2+ .

3 . The probe as claimed in claim 1 , further comprising a chelating agent capped on the surface of the gold nanocluster.

4 . The probe as claimed in claim 3 , wherein the chelating agent comprises glutathione, and the probe is used to detect Ag + , Gd 3+ , Al 3+ , Zr 4+ , Fe 3+ , Cd 2+ , Ga 3+ , Pb 2+ , Eu 3+ , or Cu 2+ .

5 . The probe as claimed in claim 3 , wherein the chelating agent comprises N—[Nα,Nα-Bis(carboxymethyl)-L-lysine]-12-mercaptododecanamide, and the probe is used to detect Co 2+ , Ni 2+ , Ag + , Gd 3+ , Al 3+ , Zr 4+ , Zn 2+ , Fe 3+ , Cd 2+ , Ga 3+ , Pb 2+ , Eu 3+ , or Cu 2+ .

6 . The probe as claimed in claim 3 , further comprising metal ions chelated to the reducing agent and the chelating agent, and the probe is used to detect chemical/biochemical molecules.

7 . The probe as claimed in claim 6 , wherein the reducing agent comprises glutathione, the chelating agent comprises glutathione, the metal ions comprise Gd 3+ , and the chemical/biochemical molecules contain phosphate groups.

8 . The probe as claimed in claim 1 , further comprising metal ions chelated to the reducing agent, and the probe is used to detect chemical/biochemical molecules.

9 . The probe as claimed in claim 8 , wherein the reducing agent comprises glutathione, the metal ions comprise Gd 3+ , and the chemical/biochemical molecules contain phosphate groups.

10 . A method for detecting metal ions, comprising:
providing a probe, wherein the probe includes: a gold nanocluster; and a reducing agent partially capped on a surface of the gold nanocluster, wherein the probe is formed of reducing gold ions by the reducing agent, and the gold ions and the reducing agent have a molar ratio of 1:0.7 to 1:1.9; and mixing the probe and an analyte solution to form a mixture, and analyzing the analyte solution to determine whether it contains specific metal ions or not by comparing fluorescent spectra difference of the probe and the mixture.

11 . The method as claimed in claim 10 , wherein the reducing agent comprises glutathione, and the metal ions comprise Ag + , Gd 3+ , Zr 4+ , Fe 3+ , Ga 3+ , Pb 2+ , Eu 3+ , or Cu 2+ .

12 . The method as claimed in claim 10 , further comprising a chelating agent capped on the surface of the gold nanocluster.

13 . The method as claimed in claim 12 , wherein the reducing agent comprises glutathione, the chelating agent comprises glutathione, and the metal ions comprise Ag + , Gd 3+ , Al 3+ , Zr 4+ , Fe 3+ , Cd 2+ , Ga 3+ , Pb 2+ , Eu 3+ , or Cu 2+ .

14 . The method as claimed in claim 12 , wherein the reducing agent comprises glutathione, the chelating agent comprises N—[Nα,Nα-Bis(carboxymethyl)-L-lysine]-12-mercaptododecanamide, and the metal ions comprise Co 2+ , Ni 2+ , Ag + , Gd 3+ , Al 3+ , Zr 4+ , Zn 2+ , Fe 3+ , Cd 2+ , Ga 3+ , Pb 2+ , Eu 3+ , or Cu 2+ .

15 . A method for detecting chemical/biochemical molecules, comprising:
providing a probe, wherein the probe includes: a gold nanocluster; a reducing agent partially capped on a surface of the gold nanocluster; and metal ions chelated to the reducing agent, wherein the probe is formed of reducing gold ions by the reducing agent, and the gold ions and the reducing agent have a molar ratio of 1:0.7 to 1:1.9; and mixing the probe and an analyte solution to form a mixture, and analyzing the analyte solution to determine whether it contains specific chemical/biochemical molecules or not by comparing fluorescent spectra difference of the probe and the mixture.

16 . The method as claimed in claim 15 , wherein the reducing agent comprises glutathione, the metal ions comprise Gd 3+ , and the chemical/biochemical molecules contain phosphate groups.

17 . The method as claimed in claim 15 , further comprising a chelating agent capped on the surface of the gold nanocluster, and the metal ions are also chelated to the chelating agent.

18 . The method as claimed in claim 17 , wherein the reducing agent comprises glutathione, the chelating agent comprises glutathione, the metal ions comprise Gd 3+ , and the chemical/biochemical molecules contain phosphate groups.

Assignees

• Ind Tech Res Inst

Inventors

• Chen Cheng-Tai
• Jiang Pei-Shin

Classifications

• G01N33/20 Primary

  Metals · CPC title

• G01N31/22 Primary

  using chemical indicators (G01N31/02 takes precedence) · CPC title

• G01N33/84

  involving inorganic compounds or pH · CPC title

• G01N33/587

  Nanoparticles · CPC title

• G01N33/553

  Metal or metal coated · CPC title