Novel use of maltotriosyl transferase

Patent metadata

Field	Value
Publication number	US-2016177356-A1
Application number	US-201615063962-A
Country	US
Kind code	A1
Filing date	Mar 8, 2016
Priority date	Feb 4, 2011
Publication date	Jun 23, 2016
Grant date	—

Abstract

The present invention is intended to provide a novel use of a maltotriosyl transferase. The present invention provides a method for producing rice cakes or noodles, including a step of heat-treating a dough containing maltotriosyl transferase thereby gelatinizing starch in the dough. The present invention also provides a method for producing an indigestible saccharide, including a step of allowing maltotriosyl transferase to act on a saccharide.

First claim

1 . A method for producing an indigestible saccharide, comprising a step of allowing maltotriosyl transferase to act on a dextrin, wherein the maltotriosyl transferase is composed of an amino acid sequence SEQ ID NO. 3, an amino acid sequence which is 70% or more identical to the amino acid sequence SEQ ID NO. 3, or a fragment of the amino acid sequence SEQ ID NO. 3 showing maltotriosyl transferase activity.

2 . The method according to claim 1 , wherein the step is carried out using one or more enzymes selected from the group consisting of cyclodextrin glucanotransferase, a-amylase (only those having transglycosylation activity), pullulanase, and isoamylase in combination with the maltotriosyl transferase.

3 . The production method according to claim 1 , wherein the reaction temperature during the step is from 50° C. to 70° C.

4 . The method for producing an indigestible saccharide according to claim 1 , wherein the step is carried out in the presence of a receptor substrate, and a hetero-oligosaccharide is formed as the indigestible saccharide.

5 . The method for producing an indigestible saccharide according to claim 4 , wherein the receptor substrate is monosaccharide and/or sugar alcohol.

6 . The method for producing an indigestible saccharide according to claim 4 , wherein the receptor substrate is one or more sugars or sugar alcohols selected from the group consisting of D-xylose, D-fructose, D-glucose, L-fucose, L-sorbose, Dmannose, D-sorbitol, D-galactose, N-acetyl glucosamine, L-arabinose, D-ribose, and Lrhamnose.

7 . The method for producing an indigestible saccharide according to claim 1 , wherein the dextrin is formed by saccharification of a saccharide ingredient used for producing beer or a beer-like beverage.

8 . The method for producing an indigestible saccharide according to claim 7 , wherein the saccharide ingredient is one or more ingredients selected from the group consisting of malt, barley, wheat, rye, oat, corn, rice, kaoliang, potato, soybean, pea bean, chick-pea, and corn starch.

9 . The production method according to claim 1 , wherein the maltotriosyl transferase has the following enzymatic properties:
the enzyme acts on polysaccharides and oligosaccharides having a-1,4 glucoside bonds to transfer maltotriose units to saccharides; when the enzyme acts on maltotetraose as the substrate, the ratio between the production rates of maltoheptaose and maltotriose is from 9:1 to 10:0 over the whole range of the substrate concentration from 0.67% (w/v) to 70% (w/v).

10 . The production method according to claim 1 , wherein the maltotriosyl transferase is an enzyme derived from a microorganism.

11 . The production method according to claim 1 , wherein the maltotriosyl transferase is an enzyme derived from Geobacillus species.

12 . The production method according to claim 11 , wherein the Geobacillus microorganism is Geobacillus sp. APC9669 (accession number NITE BP-770).

13 . The production method according to claim 1 , wherein the maltotriosyl transferase has the following enzymatic properties:
(1) action: the enzyme acts on polysaccharides and oligosaccharides having a-1,4 glucoside bonds to transfer maltotriose units to saccharides; (2) substrate specificity: the enzyme acts on soluble starch, amylose, amylopectin, maltotetraose, maltopentaose, and maltohexaose, but does not act on a-cyclodextrin, b-cyclodextrin, g-cyclodextrin, maltotriose, and maltose; and (3) molecular weight: about 83,000 (by SDS-PAGE).

14 . A composition containing an indigestible saccharide produced by the production method according to claim 1 .

15 . The composition according to claim 14 , which is a pharmaceutical composition, a quasi-drug composition, or a food composition.

16 . The composition according to claim 15 , wherein the food composition is beer or a beer-like beverage.

17 . The method for producing an indigestible saccharide according to claim 1 , wherein the maltotriosyl transferase has the following enzymatic properties:
the enzyme acts on polysaccharides and oligosaccharides having a-1,4 glucoside bonds to transfer maltotriose units to saccharides; when the enzyme acts on maltotetraose as the substrate, the ratio between the production rates of maltoheptaose and maltotriose is from 9:1 to 10:0 over the whole range of the substrate concentration from 0.67% (w/v) to 70% (w/v).

18 . A method for producing a dextrin solution which is hard to retrograde, comprising a step of allowing maltotriosyl transferase to act on dextrin in a solution.

19 . A dextrin solution produced by the production method according to claim 18 .

20 . A composition comprising the dextrin solution according to claim 19 .

Assignees

• Amano Enzyme Inc

Inventors

• Okada Masamichi
• Yamaguchi Shotaro

Classifications

• C12C7/047

  part of the mash being unmalted cereal mash · CPC title

• C12P19/04 Primary

  Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds · CPC title

• C12C12/002

  using special microorganisms · CPC title

• C12C5/004

  Enzymes · CPC title

• A23L33/21

  Addition of substantially indigestible substances, e.g. dietary fibres · CPC title