Clubroot resistant brassica oleracea plants

Patent metadata

Field	Value
Publication number	US-2016177331-A1
Application number	US-201615057684-A
Country	US
Kind code	A1
Filing date	Mar 1, 2016
Priority date	Jul 26, 2002
Publication date	Jun 23, 2016
Grant date	—

Abstract

The present invention discloses B. oleracea plants resistant to clubroot disease. In particular, the plants of the present invention comprise a monogenic dominant resistance to the disease clubroot introgressed from B. rapa . This resistance provides improved resistance to the disease as compared to previously existing resistances in B. oleracea.

First claim

What is claimed is:

1 . A Brassica oleracea plant resistant to clubroot disease, wherein the resistance to clubroot is monogenic and dominant and is obtainable from a clubroot resistant Brassica rapa plant, wherein said B. rapa plant is Chinese cabbage F1 hybrid Parkin.

2 . The plant according to claim 1 , wherein said B. oleracea plant is rated at level 2 or less in a test for the disease having a 1-9 scale or at a level 1 or less in a test for the disease having a 0-5 scale.

3 . The plant according to claim 1 , wherein said B. oleracea plant is rated at level 1 in a test for the disease having a 1-9 scale or at a level 0 in a test for the disease having a 0-5 scale.

4 . The plant according to claim 1 , wherein said B. oleracea plant is broccoli, white cabbage, cauliflower, Brussels sprouts, Borecole, Savoy, or red cabbage.

5 . The plant according to claim 1 , wherein said resistance is linked to a molecular marker obtainable by PCR amplification using primer O20 (SEQ ID NO:1) or primer Y13 (SEQ ID NO:2).

6 . The plant according to claim 1 , wherein said B. oleracea plant is homozygous for said resistance.

7 . The plant according to claim 1 , wherein said B. oleracea plant is heterozygous for said resistance.

8 . The plant according to claim 1 , wherein said B. oleracea plant is a hybrid, an inbred or a dihaploid.

9 . The plant according to claim 8 , wherein said B. oleracea plant is cytoplasmic male sterile.

10 . Seed of a plant according to claim 1 .

11 . Fruit or part of a plant according to claim 1 .

12 . Part of a plant according to claim 1 , wherein said part is pollen, ovule or embryo.

13 . A method for transferring a monogenic and dominant resistance to clubroot to a B. oleracea plant susceptible or less resistant to the disease comprising the steps of:
a) obtaining a B. oleracea plant according to claim 1 comprising a monogenic and dominant resistance to clubroot; b) crossing said B. oleracea plant of step a) with a B. oleracea plant susceptible or less resistant to clubroot; c) selecting a plant from the cross of step b) that is resistant to clubroot.

14 . The method according to claim 13 , further comprising backcrossing said resistance into said B. oleracea plant susceptible or less resistant to clubroot.

15 . A method of identifying a B. oleracea plant resistant to clubroot comprising the steps of:
a) obtaining a sample from a B. oleracea plant; b) detecting in said sample a DNA fragment obtainable by PCR amplification using primer O20 (SEQ ID NO:1) or primer Y13 (SEQ ID NO:2),
wherein said B. oleracea plant of step b) is resistant to clubroot.

16 . A method of selecting a B. oleracea plant resistant to clubroot from a population of B. oleracea plants comprising the steps of:
a) providing a population of B. oleracea plants; b) obtaining a sample of a plant of said population; c) detecting in said sample a DNA fragment obtainable by PCR amplification using primer O20 (SEQ ID NO:1) or primer Y13 (SEQ ID NO:2),
wherein said B. oleracea plant of step b) is resistant to clubroot.

Assignees

• Syngenta Participations Ag

Inventors

• Linders Enrico Gerardus Albertus
• Tjeertes Peter
• De Haas Johannes Maria
• Huang Cai-Cheng

Classifications

• C12N15/8279 Primary

  for biotic stress resistance, pathogen resistance, disease resistance · CPC title

• C12Q2600/13

  Plant traits · CPC title

• C12Q1/6895

  for plants, fungi or algae · CPC title

• A01H1/045

  using molecular markers · CPC title

• A01H4/00

  Plant reproduction by tissue culture techniques {; Tissue culture techniques therefor} · CPC title