Acetogenic bacteria

Patent metadata

Field	Value
Publication number	US-2016177259-A1
Application number	US-201514969891-A
Country	US
Kind code	A1
Filing date	Dec 15, 2015
Priority date	Dec 22, 2014
Publication date	Jun 23, 2016
Grant date	—

Abstract

A method of culturing at least one acetogenic bacteria, includes growing the bacteria in an aqueous medium comprising an amount of cysteine and/or cystine, wherein the cysteine and/or cystine concentration in the medium is maintained at a concentration of less than 0.20 g/L.

First claim

1 . A method of culturing at least one acetogenic bacteria, the method comprising:
growing the bacteria in an aqueous medium comprising an amount of cysteine, cystine or mixtures thereof; wherein a concentration of the cysteine, cystine or mixtures thereof in the medium is maintained at a concentration range of 0.05 g/L to 0.20 g/L.

2 . The method according to claim 1 , wherein the concentration of cysteine, cystine or mixtures thereof in the medium is maintained at about 0.10 g/L.

3 . The method according to claim 1 , wherein the concentration of cysteine, cystine or mixtures thereof in the medium is maintained at about 0.08 g/L.

4 . The method according to claim 1 , wherein the medium further comprises a carbon source comprising CO, CO 2 or mixtures thereof.

5 . The method according to claim 4 , wherein the carbon source comprises at least 50% by weight of CO, CO 2 or mixtures thereof.

6 . The method according to to claim 1 , wherein the acetogenic bacteria is selected from the group consisting of Acetoanaerobium notera (ATCC 35199), Acetonema longum (DSM 6540), Acetobacterium carbinolicum (DSM 2925), Acetobacterium malicum (DSM 4132), Acetobacterium species no. 446, Acetobacterium wieringae (DSM 1911), Acetobacterium woodii (DSM 1030), Alkalibaculum bacchi (DSM 22112), Archaeoglobus fulgidus (DSM 4304), Blautia producta (DSM 2950), Butyribacterium methylotrophicum (DSM 3468), Clostridium aceticum (DSM 1496), Clostridium autoethanogenum (DSM 10061, DSM 19630 and DSM 23693), Clostridium carboxidivorans (DSM 15243), Clostridium coskatii (ATCC no. PTA-10522), Clostridium drakei (ATCC BA-623), Clostridium formicoaceticum (DSM 92), Clostridium glycolicum (DSM 1288), Clostridium ljungdahlii (DSM 13528), Clostridium ljungdahlii C-01 (ATCC 55988), Clostridium ljungdahlii ERI-2 (ATCC 55380), Clostridium ljungdahlii O-52 (ATCC 55989), Clostridium mayombei (DSM 6539), Clostridium methoxybenzovorans (DSM 12182), Clostridium ragsdalei (DSM 15248), Clostridium scatologenes (DSM 757), Clostridium species ATCC 29797, Desulfotomaculum kuznetsovii (DSM 6115), Desulfotomaculum thermobezoicum subsp. thermosyntrophicum (DSM 14055), Eubacterium limosum (DSM 20543), Methanosarcina acetivorans C2A (DSM 2834), Moorella sp. HUC22-1, Moorella thermoacetica (DSM 521), Moorella thermoautotrophica (DSM 1974), Oxobacter pfennigii (DSM 322), Sporomusa aerivorans (DSM 13326), Sporomusa ovata (DSM 2662), Sporomusa silvacetica (DSM 10669), Sporomusa sphaeroides (DSM 2875), Sporomusa termitida (DSM 4440), Thermoanaerobacter kivui (DSM 2030) and combinations thereof.

7 . A method of producing acetate, ethanol or mixtures thereof from at least one carbon source, the method comprising:
growing at least one acetogenic bacteria in an aqueous medium comprising an amount of cysteine, cystine or mixtures thereof; wherein a concentration of cysteine, cystine or mixtures thereof in the medium is maintained at a concentration range of 0.05 g/L to 0.20 g/L; and obtaining said acetate, ethanol or mixtures thereof.

8 . The method according to claim 7 , wherein the concentration of cysteine, cystine or mixtures thereof in the medium is maintained at about 0.10 g/L.

9 . The method according to claim 7 , wherein the concentration of cysteine and/or cystine in the medium is maintained at about 0.08 g/L.

10 . The method according to claim 7 , wherein the carbon source comprises at least 50% by weight of CO, CO 2 . and mixtures thereof.

11 . The method according to claim 7 , wherein the acetogenic bacteria is selected from the group consisting of Acetoanaerobium notera (ATCC 35199), Acetonema longum (DSM 6540), Acetobacterium carbinolicum (DSM 2925), Acetobacterium malicum (DSM 4132), Acetobacterium species no. 446, Acetobacterium wieringae (DSM 1911), Acetobacterium woodii (DSM 1030), Alkalibaculum bacchi (DSM 22112), Archaeoglobus fulgidus (DSM 4304), Blautia producta (DSM 2950), Butyribacterium methylotrophicum (DSM 3468), Clostridium aceticum (DSM 1496), Clostridium autoethanogenum (DSM 10061, DSM 19630 and DSM 23693), Clostridium carboxidivorans (DSM 15243), Clostridium coskatii (ATCC no. PTA-10522), Clostridium drakei (ATCC BA-623), Clostridium formicoaceticum (DSM 92), Clostridium glycolicum (DSM 1288), Clostridium ljungdahlii (DSM 13528), Clostridium ljungdahlii C-01 (ATCC 55988), Clostridium ljungdahlii ERI-2 (ATCC 55380), Clostridium ljungdahlii O-52 (ATCC 55989), Clostridium mayombei (DSM 6539), Clostridium methoxybenzovorans (DSM 12182), Clostridium ragsdalei (DSM 15248), Clostridium scatologenes (DSM 757), Clostridium species ATCC 29797, Desulfotomaculum kuznetsovii (DSM 6115), Desulfotomaculum thermobezoicum subsp. thermosyntrophicum (DSM 14055), Eubacterium limosum (DSM 20543), Methanosarcina acetivorans C2A (DSM 2834), Moorella sp. HUC22-1, Moorella thermoacetica (DSM 521), Moorella thermoautotrophica (DSM 1974), Oxobacter pfennigii (DSM 322), Sporomusa aerivorans (DSM 13326), Sporomusa ovata (DSM 2662), Sporomusa silvacetica (DSM 10669), Sporomusa sphaeroides (DSM 2875), Sporomusa termitida (DSM 4440), Thermoanaerobacter kivui (DSM 2030) and combinations thereof.

12 . The method according to claim 7 , wherein the cysteine, cystine or mixtures thereof present in the medium is added as a compound selected from the group consisting of L-cysteine-HCl, L-Cysteine, L-Cysteine-HCl (free of water) and L-Cysteine*H 2 O and mixtures thereof.

13 . A method of fermenting syngas to produce acetate, ethanol or mixtures thereof, the method comprising:
culturing at least one acetogenic bacteria in an aqueous medium comprising an amount of cysteine, cystine or mixtures thereof; wherein the cysteine, cystine or mixtures thereof is maintained at a concentration range of 0.05 g/L to 0.20 g/L.

14 . The method according to claim 13 , wherein the concentration of cysteine, cystine or mixtures thereof in the medium is maintained at about 0.10 g/L.

15 . The method according to claim 13 , wherein the acetogenic bacteria is selected from the group consisting of Acetoanaerobium notera (ATCC 35199), Acetonema longum (DSM 6540), Acetobacterium carbinolicum (DSM 2925), Acetobacterium malicum (DSM 4132), Acetobacterium species no. 446, Acetobacterium wieringae (DSM 1911), Acetobacterium woodii (DSM 1030), Alkalibaculum bacchi (DSM 22112), Archaeoglobus fulgidus (DSM 4304), Blautia producta (DSM 2950), Butyribacterium methylotrophicum (DSM 3468), Clostridium aceticum (DSM 1496), Clostridium autoethanogenum (DSM 10061, DSM 19630 and DSM 23693), Clostridium carboxidivorans (DSM 15243), Clostridium coskatii (ATCC no. PTA-10522), Clostridium drakei (ATCC BA-623), Clostridium formicoaceticum (DSM 92), Clostridium glycolicum (DSM 1288), Clostridium ljungdahlii (DSM 13528), Clostridium ljungdahlii C-01 (ATCC 55988), Clostridium ljungdahlii ERI-2 (ATCC 55380), Clostridium ljungdahlii O-52 (ATCC 55989), Clostridium mayombei (DSM 6539), Clostridium methoxybenzovorans (DSM 12182), Clostridium ragsdalei (DSM 15248), Clostridium scatologenes (DSM 757), Clostridium species ATCC 29797, Desulfotomaculum kuznetsovii (DSM 6115), Desulfotomaculum thermobezoicum subsp. thermosyntrophicum (DSM 14055), Eubacterium limosum (DSM 20543), Methanosarcina acetivorans C2A (DSM 2834), Moorella sp. HUC22-1, Moorella thermoacetica (DSM 521), Moorella thermoautotrophica (DSM 1974), Oxobacter pfennigii (DSM 322), Sporomusa aerivorans (DSM 13326), Sporomusa ovata (DSM 2662), Spo

Assignees

• Haas Thomas
• Buelter Thomas
• Demler Martin
• Thiessenhusen Anja
• Evonik Degussa Gmbh

Inventors

• Haas Thomas
• Buelter Thomas
• Demler Martin
• Thiessenhusen Anja

Classifications

• C12P7/065

  with microorganisms other than yeasts · CPC title

• C12P7/24

  containing a carbonyl group · CPC title

• C12N1/20 Primary

  Bacteria; Culture media therefor · CPC title

• C12P7/54

  Acetic acid (vinegar C12J) · CPC title

• Y02E50/10

  Biofuels, e.g. bio-diesel · CPC title