Methods for the production of collagen iv

Patent metadata

Field	Value
Publication number	US-2016175347-A1
Application number	US-201414905457-A
Country	US
Kind code	A1
Filing date	Jul 14, 2014
Priority date	Jul 15, 2013
Publication date	Jun 23, 2016
Grant date	—

Abstract

The method of the current disclosure provides for methods of increasing sulfilimine bonds in collagen IV using bromide containing agents and conditions that either promote peroxidasin cross-linking of collagen IV.

First claim

1 . A method for promoting tissue stability in a target tissue site in a subject in need thereof comprising:
(a) identifying a subject in need of tissue stabilization; and (b) administering to said subject a bromide salt.

2 . The method of claim 1 , wherein said subject is a non-human animal.

3 . The method of claim 1 , wherein said subject is a human.

4 . The method of claim 1 , wherein said bromide salt is administered as a sole active ingredient.

5 . The method of claim 1 , wherein said bromide salt is administered in combination with a second active agent.

6 . The method of claim 5 , wherein said second active agent is peroxide, molecular oxygen, electron-accepting compound such as flavin adenine dinucleotide (FAD), hypobromous acid, nicotinamide adenine dinucelotide (NAD & NADH), nicotinamide adenine dinucelotide phosphate (NADP & NADPH), inosine monophosphate (IMP), guanosine monophosphate (GMP) or a combination thereof.

7 . The method of claim 1 , wherein the target concentration of bromide at said target tissue site is between 1 μM and 1 mM following treatment, such as 5 μm, 10 μm, 15 μm, 20 μm, 25 μm, 30 μm, 50 μm, 75 μm, 100 μm, 150 μm, 200 μm, 250 μm, 300 μm, 400 μm, 500 μm, 600 μm, 700 μm, 800 μm, or 900 μm.

8 . The method of claim 1 , wherein said subject suffers from loss, removal, weakening or injury of collagen containing tissues.

9 . The method of claim 8 , wherein loss, removal, weakening or injury involves a medical operation, a trauma, a disease, natural aging, or exposure to an environmental factor.

10 . The method of claim 9 , where the disease is periodontal disease or cataracts.

11 . The method of claim 1 , wherein said second active agent is an antimicrobial agent.

12 . The method of claim 11 , wherein the antimicrobial agent is a hypohalous acid or hypohalite compound.

13 . The method of claim 1 , further comprising reduce the amount of chloride or thiocyanate levels in the blood or at said target tissue site in said subject.

14 . The method of claim 1 , wherein administering comprises oral, intravenous, intra-arterial, subcutaneous, transdermal or topical administration.

15 . The method of claim 1 , wherein administering comprises systemic administration or administration to or local/regional to a site of healing.

16 . A method for predicting the ability of a subject's tissue to recover or heal comprising measuring the concentration of ionic bromide in a sample from said subject, wherein a bromide blood concentration between 1 μM and 1 mM indicates normal healing and recovery as compared to a normal subject, and a bromide blood concentration above or below 1 μM and 1 mM indicates reduced or delayed healing or recovery as compared to a normal subject.

17 - 25 . (canceled)

26 . A method for promoting sulfilimine bond formation in tissue in culture comprising contacting a collagen-containing tissue with a bromide salt.

27 - 35 . (canceled)

36 . A method of improving tissue development in pre-natal or post-natal infant comprising administering to said infant a bromide salt.

37 - 50 . (canceled)

Assignees

• Univ Vanderbilt

Inventors

• Hudson Billy G
• Cummings Christopher F
• Bhave Gautam
• Mccall A Scott

Classifications

• A61K33/40

  Peroxides · CPC title

• A61K31/7084

  Compounds having two nucleosides or nucleotides, e.g. nicotinamide-adenine dinucleotide, flavine-adenine dinucleotide · CPC title

• A61K31/708

  having oxo groups directly attached to the purine ring system, e.g. guanosine, guanylic acid · CPC title

• A61K45/06

  Mixtures of active ingredients without chemical characterisation, e.g. antiphlogistics and cardiaca · CPC title

• G01N33/49

  Blood {(chemical methods for determining blood cell populations G01N33/5094; chemical analysis of blood groups or blood types G01N33/80)} · CPC title