Polypeptides Having Beta-1,3-Galactanase Activity and Polynucleotides Encoding Same

Patent metadata

Field	Value
Publication number	US-2016174589-A1
Application number	US-201414909579-A
Country	US
Kind code	A1
Filing date	Aug 15, 2014
Priority date	Aug 15, 2013
Publication date	Jun 23, 2016
Grant date	—

Abstract

The present invention relates to polypeptides having beta-1,3-galactanase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

First claim

1 . A polypeptide having beta-1,3-galactanase activity, selected from the group consisting of:
(a) a polypeptide having at least 65% sequence identity to the mature polypeptide of SEQ ID NO: 2; (b) a polypeptide encoded by a polynucleotide that hybridizes under low stringency conditions with the mature polypeptide coding sequence of SEQ ID NO: 1 or the full-length complement hereof; (c) a polypeptide encoded by a polynucleotide having at least 65% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 1; (d) a variant of the mature polypeptide of SEQ ID NO: 2 comprising a substitution, deletion, and/or insertion at one or more positions; and (e) a fragment of the polypeptide of (a), (b), (c), or (d) that has beta-1,3-galactanase activity.

2 . The polypeptide of claim 1 , which is an isolated polypeptide.

3 . The polypeptide of claim 1 , having at least 65% sequence identity to the mature polypeptide of SEQ ID NO: 2.

4 . The polypeptide of claim 1 , which is encoded by a polynucleotide that hybridizes under low stringency conditions, low-medium stringency conditions, medium stringency conditions, medium-high stringency conditions, high stringency conditions, or very high stringency conditions with the mature polypeptide coding sequence of SEQ ID NO: 1 or the full-length complement hereof.

5 . The polypeptide of claim 1 , which is encoded by a polynucleotide having at least 65% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 1.

6 . The polypeptide of claim 1 , comprising or consisting of SEQ ID NO: 2 or the mature polypeptide of SEQ ID NO: 2.

7 . The polypeptide of claim 1 , wherein the mature polypeptide is amino acids 21 to 256 of SEQ ID NO: 2.

8 . A composition comprising the polypeptide of claim 1 .

9 . (canceled)

10 . An isolated polynucleotide encoding the polypeptide of claim 1 .

11 . A nucleic acid construct or expression vector comprising the polynucleotide of claim 10 operably linked to one or more control sequences that direct the production of the polypeptide in an expression host.

12 . A recombinant host cell comprising the polynucleotide of claim 10 operably linked to one or more control sequences that direct the production of the polypeptide.

13 . A method of producing a polypeptide having beta-1,3-galactanase activity, comprising cultivating the host cell of claim 12 under conditions conducive for production of the polypeptide.

14 . The method of claim 13 , further comprising recovering the polypeptide.

15 . A whole broth formulation or cell culture composition comprising a polypeptide of claim 1 .

16 . A method or producing a coffee extract, comprising the steps:
a. providing roast and ground coffee beans; b. adding to said coffee beans water and a polypeptide of claim 1 ; c. incubating to make an aqueous coffee extract; and d. separating the coffee extract from the extracted coffee beans.

Assignees

• Novozymes As

Inventors

• Spodsberg Nikolaj
• Krogh Kristian Bertel Roemer M
• Monrad Rune Nygaard

Classifications

• A23F5/246 Primary

  Addition of, or treatment with, enzymes or microorganisms (A23F5/163 and A23F5/204 take precedence) · CPC title

• C12N9/2468

  acting on beta-galactose-glycoside bonds, e.g. carrageenases (3.2.1.83; 3.2.1.157); beta-agarase (3.2.1.81) · CPC title

• C12Y302/01181

  Galactan endo-beta-1,3-galactanase (3.2.1.181) · CPC title

• A23F5/28

  Drying or concentrating coffee extract {(A23F5/246 takes precedence)} · CPC title