Cell culturing method, cell culturing apparatus and kit

Patent metadata

Field	Value
Publication number	US-2016168560-A1
Application number	US-201414907432-A
Country	US
Kind code	A1
Filing date	Jul 25, 2014
Priority date	Jul 26, 2013
Publication date	Jun 16, 2016
Grant date	—

Abstract

The present invention relates to a cell culturing method as well as a cell culturing apparatus and kit for use in said culturing method. This cell culturing method comprises applying cells on a porous polyimide film and culturing. One embodiment of the method according to the present invention comprises a process for sowing cells on the surface of the porous polyimide film. An embodiment comprises a process for placing a cell suspension on the dried surface of the porous polyimide film and leaving the porous polyimide film undisturbed or moving said porous polyimide film to promote liquid efflux or stimulating a portion of the surface to cause the cell suspension to be entangled into the film and then retaining the cells in the cell suspension inside the film while allowing the moisture to flow out. Another embodiment comprises a process for moistening one or both surfaces of the porous polyimide film with a cell culture solution or sterilized liquid, loading a cell suspension on the moistened porous polyimide film, then retaining the cells in the cell suspension inside the film, and allowing the moisture to flow out.

First claim

1 . A cell culturing method that includes applying cells to a porous polyimide film and culturing them.

2 . The method according to claim 1 , including a step of seeding cells on the surface of a porous polyimide film.

3 . The method according to claim 1 , including a step of:
placing a cell suspension on the dried surface of the porous polyimide film, allowing the porous polyimide film to stand, or moving the porous polyimide film to promote efflux of the liquid, or stimulating part of the surface to cause absorption of the cell suspension into the film, and retaining the cells in the cell suspension inside the film and allowing the water to flow out.

4 . The method according to claim 1 , including a step of:
wetting one or both sides of the porous polyimide film with a cell culture medium or a sterilized liquid, loading a cell suspension into the wetted porous polyimide film, and retaining the cells in the cell suspension inside the film and allowing the water to flow out.

5 . The method according to claim 4 , wherein the viable cells are retained in the porous polyimide film, and the dead cells are allowed to flow out together with the water.

6 . The method according to claim 4 , wherein the sterilized liquid is sterilized water or a sterilized buffering solution.

7 . The method according to claim 1 , including the aspect that the cell culture medium, cells and one or more porous polyimide films are placed in a cell culturing vessel, wherein the porous polyimide film is in a suspended state in the cell culture medium.

8 . The method according to claim 7 , wherein two or more fragments of the porous polyimide film are used.

9 . The method according to claim 7 , wherein the cells spontaneously adhere to the porous polyimide film.

10 . The method according to claim 1 , wherein the porous polyimide film is:
i) folded, ii) wound into a roll, iii) connected as sheets or fragments by a filamentous structure, or iv) bound into a rope,
for suspension or fixing in the cell culture medium in the cell culturing vessel.

11 . The method according to claim 10 , wherein the cells spontaneously adhere to the porous polyimide film.

12 . The method according to claim 1 , including using two or more porous polyimide films layered either above and below or left and right in the cell culture medium.

13 . (canceled)

14 . The method according to claim 1 , wherein the cells grow and proliferate on the surface of and inside the porous polyimide film.

15 . The method according to claim 1 , wherein the cells are selected from the group consisting of animal cells, insect cells, plant cells, yeast cells and bacteria.

16 . The method according to claim 15 , wherein the animal cells are cells derived from an animal belonging to the subphylum Vertebrata.

17 . The method according to claim 15 , wherein the bacteria are selected from the group consisting of lactic acid bacteria, E. coli, Bacillus subtilis and cyanobacteria.

18 . The method according to claim 1 ,
wherein the cells are selected from the group consisting of pluripotent stem cells, tissue stem cells, somatic cells and germ cells.

19 . The method according to claim 1 , wherein the cells are selected from the group consisting of sarcoma cells, established cell lines and transformants.

20 . The method according to claim 1 , wherein the porous polyimide film is a porous polyimide film including a polyimide obtained from a tetracarboxylic dianhydride and a diamine.

21 . A cell culturing apparatus for use in a cell culturing method according to claim 1 , including a porous polyimide film.

22 . A cell culturing apparatus according to claim 21 , wherein two or more porous polyimide films are layered either above and below or left and right.

23 . A kit for use in a cell culturing method according to claim 1 , including a porous polyimide film.

Assignees

• Ube Industries

Inventors

• Hagihara Masahiko
• Kawaguchi Tetsuo
• Baba Kousuke
• Shimizu Motohisa

Classifications

• C12N11/08 Primary

  the carrier being a synthetic polymer · CPC title

• C12N11/089

  obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds · CPC title

• C12N2533/30

  Synthetic polymers · CPC title

• C12N5/0068 Primary

  General culture methods using substrates (for specific animal cell type C12N5/06) · CPC title

• C12N1/20

  Bacteria; Culture media therefor · CPC title