High efficiency microfluidic purification of stem cells to improve transplants

Patent metadata

Field	Value
Publication number	US-2016168539-A1
Application number	US-201514941957-A
Country	US
Kind code	A1
Filing date	Nov 16, 2015
Priority date	Mar 15, 2013
Publication date	Jun 16, 2016
Grant date	—

Abstract

Described herein is a novel, highly efficient system to remove erythrocytes and purify leukocytes would raise the quality of UCB and other transplant grafts, thereby significantly improving patient outcomes.

First claim

What is claimed is:

1 . A method for isolating stem cells from a sample for transplantation, the method comprising:
(a) providing a sample comprising erythrocytes and leukocytes, the sample having a volume of less than 300 mL; (b) depleting the erythrocytes; and (c) enriching the leukocytes to a purity of at least 90%.

2 . The method of claim 1 , wherein the sample is umbilical cord blood.

3 . The method of claim 2 , wherein the umbilical cord blood is not cryopreserved.

4 . The method of claim 1 , further comprising using the stem cells in a transplantation procedure.

5 . The method of claim 1 , wherein the yield of leukocytes is at least 90%.

6 . The method of claim 1 , wherein the viability of the leukocytes is at least 90%.

7 . The method of claim 1 , wherein the method is performed in less than 1 hour.

8 . The method of claim 1 , wherein the method is performed in less than 10 minutes.

9 . The method of claim 1 , wherein the sample has at least 1000-fold more erythrocytes than leukocytes.

10 . The method of claim 1 , wherein the method does not use centrifugation, Ficoll-Paque or HES

11 . The method of claim 1 , wherein neither the erythrocytes nor the leukocytes are derivatized or labeled.

12 . The method of claim 1 , wherein the sample is flowed through a biochip having a plurality of microscopic obstructions that sort the erythrocytes from the leukocytes.

Assignees

• Univ Maryland
• Univ Princeton

Inventors

• Civin Curt I
• Sturm James C
• Austin Robert H

Classifications

• C12N5/0647 Primary

  Haematopoietic stem cells; Uncommitted or multipotent progenitors · CPC title

• C12N5/0634 Primary

  Cells from the blood or the immune system · CPC title

• A61K35/15

  Cells of the myeloid line, e.g. granulocytes, basophils, eosinophils, neutrophils, leucocytes, monocytes, macrophages or mast cells; Myeloid precursor cells; Antigen-presenting cells, e.g. dendritic cells (presenting a specific antigen A61K39/00; therapeutic combinations of antibodies, or fragments thereof, and blood-derived cells A61K39/00) · CPC title

• A61K35/17

  Lymphocytes; B-cells; T-cells; Natural killer cells; Interferon-activated or cytokine-activated lymphocytes (when activated by a specific antigen A61K39/00) · CPC title

• C12N5/0087

  Purging against subsets of blood cells, e.g. purging alloreactive T cells · CPC title