Spatial Positioning of Spectrally Labeled Beads

Patent metadata

Field	Value
Publication number	US-2016161398-A1
Application number	US-201615046623-A
Country	US
Kind code	A1
Filing date	Feb 18, 2016
Priority date	Sep 24, 1998
Publication date	Jun 9, 2016
Grant date	—

Abstract

Devices, systems, kits, and methods for detecting and/or identifying a plurality of spectrally labeled bodies well-suited for performing multiplexed assays. By spectrally labeling the beads with materials which generate identifiable spectra, a plurality of beads may be identified within the fluid. Reading of the beads is facilitated by restraining the beads in arrays, and/or using a focused laser.

First claim

What is claimed is:

1 . A spectral label identification method comprising:
spatially restraining a first spectrally labeled body at a site in an array of sites; spatially restrained a plurality of labeled bodies behind the first body so as to define an array; dispersing a plurality of spectra from the spatially restrained bodies simultaneously across the sensor surface; and identifying the array of bodies from the spectrum, wherein the plurality of spectrally labeled bodies are simultaneously spatially restrained as an array when the first body is restrained.

2 . The method of claim 1 , wherein the array of bodies are restrained within an array of openings in a multi-well plate.

3 . The method of claim 2 , wherein the array of openings in the multi-well plate are spaced to avoid excessive overlap of the dispersed spectra such that each of the bodies can be identified from the associated spectrum.

4 . The method in claim 2 , wherein the array of openings in the multi-well plate are spaced in a staggered pattern so as to increase the array density while avoiding excessive overlap among the dispersed spectra.

5 . The method of claim 2 , wherein the array of openings in the multi-well plate are deep enough to contain a set of labeled bodies.

6 . The method of claim 5 , wherein the labeled bodies are moved into the array of openings in the multi-well plate by vacuum action.

7 . The method of claim 1 , wherein the labeled body is restrained by a restraining energy beam which is configured to restrain a single body.

8 . A spectral label identification method comprising:
spatially restraining a first spectrally labeled body at a site in an array of sites; spatially restraining a plurality of labeled bodies behind the first body so as to define an array. sensing a first and second spectra with a scanning system by moving a sensing field between the bodies of the array; and identifying the array of bodies from the spectrum, wherein the plurality of spectrally labeled bodies are simultaneously spatially restrained as an array when the first body is restrained.

9 . The method of claim 8 , wherein the array of bodies are restrained within the array of openings in a multi-well plate.

10 . The method in claim 9 , wherein the array of openings in the multi-well plate are spaced to avoid excessive overlap to dispersed spectra such that each of the bodies can be identified from the associated spectrum.

11 . The method in claim 9 , wherein the array of openings in the multi-well plate are spaced in a staggered pattern so as to increase the array density while avoiding excessive overlap among the dispersed spectra.

12 . The method of claim 9 , wherein the array of openings in a multi-well plate are deep enough to contain a set of labeled bodies.

13 . The method of claim 12 , wherein the labeled bodies are moved into the array of openings in a multi-well plate by vacuum action.

14 . A spectral label identification method comprising:
spatially restraining a first spectrally labeled body at a site in an array of sites in an array of sites; spatially restraining an array of bodies in the array by an array of discrete binging sites, the binding sites comprising material capable of binding bodies; sensing a first and second spectra with a scanning system by moving a sensing field between the bodies of the array; and identifying the array of bodies from the spectrum, wherein the plurality of spectrally labeled bodies are simultaneously spatially restrained as an array when the first body is restrained.

15 . A spectral label identification method comprising:
spatially restraining a first spectrally labeled body at a site in an array of sites in an array of sites; spatially restraining an array of bodies in the array by an array of discrete bringing sites, the binding sites comprising material capable of binding bodies; dispersing a plurality of spectra from the spatially restrained bodies simultaneously across the sensor surface; and identifying the array of bodies from the spectrum, wherein the plurality of spectrally labeled bodies are simultaneously spatially restrained as an array when the first body is restrained.

16 . A multiplex assay system comprising:
A plurality of bodies released in a fluid, the bodies having labels for generating identifiable spectra; An energy transmitter coupled to the fluid so as to spatially restrain an array of bodies with a restraining energy beam; and A sensor oriented to receive the spectrum which is simultaneously dispersed from the plurality of spatially restrained bodies across the sensor surface.

Assignees

• Life Technologies Corp

Inventors

• Empedocles Stephen
• Watson Andrew
• Jin Jian

Classifications

• B01L3/5025 Primary

  for parallel transport of multiple samples · CPC title

• G01N21/272 Primary

  for following a reaction, e.g. for determining photometrically a reaction rate (photometric cinetic analysis) · CPC title

• G01N2201/12

  Circuits of general importance; Signal processing · CPC title

• B01L2300/0819

  Microarrays; Biochips · CPC title

• G01N21/6456

  Spatial resolved fluorescence measurements; Imaging · CPC title