Pepper with novel flavor

Patent metadata

Field	Value
Publication number	US-2016160225-A1
Application number	US-201514980084-A
Country	US
Kind code	A1
Filing date	Dec 28, 2015
Priority date	Jul 2, 2013
Publication date	Jun 9, 2016
Grant date	—

Abstract

The present invention relates to a pepper plant ( Capsicum annuum L.) which may comprise a QTL that leads to the plant producing fruits that have a novel flavor, which QTL is obtainable from a pepper plant which may comprise said QTL, representative seed of which was deposited with the NCIMB under accession number NCIMB 42137.

First claim

What is claimed is:

1 . A Capsicum annuum plant that produces fruits with a novel flavor, wherein the plant comprises a QTL which when present leads to the novel flavor, wherein the QTL is located on LG3 and wherein said QTL is the same as a QTL that is present in the genome of plants grown from seeds of deposit NCIMB 42137 and is linked therein to at least one marker selected from the group consisting of SEQ ID No:1, SEQ ID No:3, SEQ ID No:5, SEQ ID No:7, SEQ ID No:9 and SEQ ID No:11.

2 . The plant of claim 1 , wherein the QTL is homozygously present.

3 . The plant of claim 1 , wherein the QTL is the same as a QTL that is present in the genome of plants grown from seeds of deposit NCIMB 42137 and is linked therein to markers SEQ ID No:1, SEQ ID No:3, SEQ ID No:5, SEQ ID No:7, SEQ ID No:9 and SEQ ID No:11.

4 . The plant of claim 1 , wherein the QTL is as present in the genome of, or obtainable from, Capsicum baccatum or a plan grown from a seed deposited at the NCIMB under accession number NCIMB 42137.

5 . The plant of claim 1 , wherein the novel flavor is defined as the pepper fruits produced by said plant having higher scores for one or more flavor attributes comprising an aroma, flower, non-pungent spice, celery and/or a chive, and/or a lower score for the flavor attribute grassiness.

6 . The plant of claim 5 comprising a higher odor intensity, as compared to pepper fruits of a similar ripening stage from isogenic pepper plants not carrying said QTL.

7 . The plant of claim 1 , wherein the fruit of the pepper plant has a higher amount of artemisia ketone and/or a higher amount of 6-methyl-4-oxo-5-heptenal and/or a higher amount of (E)-2-hexenal and/or a higher amount of (Z)-2-hexen-1-ol and/or a higher amount of 1-penten-3-ol and/or a higher amount of hexanal and/or a higher amount of (Z)-2-penten-1-ol and/or a higher amount of (E)-2-pentenal and/or a lower amount of (Z)-butanoic acid 3-hexenyl ester and/or a lower amount of 2-isobutyl-3-methoxypyrazine compared to a fruit of a similar ripening stage from an isogenic pepper plant not comprising said QTL.

8 . A seed of the plant of claim 1 , comprising the QTL of claim 1 .

9 . The seed of claim 8 , wherein the QTL is homozygously present.

10 . A seed capable of growing into the plant of claim 1 comprising the QTL of claim 1 .

11 . The seed of claim 10 , wherein the QTL is homozygously present.

12 . A progeny of the plant of claim 1 , wherein the progeny comprises the QTL of claim 1 .

13 . The progeny of claim 12 , wherein the QTL is homozygously present.

14 . A progeny of a plant grown from the seed of claim 8 , wherein the progeny comprises the QTL of claim 1 .

15 . The progeny of claim 14 , wherein the QTL is homozygously present.

16 . A progeny of a plant grown from the seed of claim 10 , wherein the progeny comprises the QTL of claim 1 .

17 . The progeny of claim 16 , wherein the QTL is homozygously present.

18 . A propagation material derived from the plant of claim 1 , wherein the propagation material comprises the QTL of claim 1 .

19 . The propagation material of claim 18 , wherein the QTL is homozygously present.

20 . A propagation material derived from the seed of claim 8 , wherein the propagation material comprises the QTL of claim 1 .

21 . The propagation material of claim 20 , wherein the QTL is homozygously present.

22 . A propagation material derived from the seed of claim 10 , wherein the propagation material comprises the QTL of claim 1 .

23 . The propagation material of claim 22 , wherein the QTL is homozygously present 24. A propagation material capable of growing into the plant of claim 1 comprising the QTL as defined in claim 1 .

25 . The propagation material of claim 24 , wherein the QTL is homozygously present.

26 . The propagation material of claim 18 , wherein the propagation material comprises a callus, microspore, pollen, ovary, ovule, embryo, embryo sac, egg cell, cutting, root, stem, cell, protoplast, leaf, cotyledon, hypocotyl, meristematic cell, root, root tip, microspore, anther, flower, seed or stem or a part or tissue culture thereof.

26 . The propagation material of claim 20 , wherein the propagation material comprises a callus, microspore, pollen, ovary, ovule, embryo, embryo sac, egg cell, cutting, root, stem, cell, protoplast, leaf, cotyledon, hypocotyl, meristematic cell, root, root tip, microspore, anther, flower, seed or stem or a part or tissue culture thereof.

26 . The propagation material of claim 22 , wherein the propagation material comprises a callus, microspore, pollen, ovary, ovule, embryo, embryo sac, egg cell, cutting, root, stem, cell, protoplast, leaf, cotyledon, hypocotyl, meristematic cell, root, root tip, microspore, anther, flower, seed or stem or a part or tissue culture thereof.

26 . The propagation material of claim 24 , wherein the propagation material comprises a callus, microspore, pollen, ovary, ovule, embryo, embryo sac, egg cell, cutting, root, stem, cell, protoplast, leaf, cotyledon, hypocotyl, meristematic cell, root, root tip, microspore, anther, flower, seed or stem or a part or tissue culture thereof.

27 . A pepper fruit, or a part thereof, from the plant of claim 1 comprising the QTL of claim 1 .

28 . The fruit of claim 27 , wherein the QTL is homozygously present.

29 . A pepper fruit, or a part thereof, from a plant grown from the seed of claim 8 comprising the QTL of claim 1 .

30 . The fruit of claim 29 , wherein the QTL is homozygously present.

31 . A pepper fruit, or a part thereof, from a plant grown from the seed of claim 10 comprising the QTL of claim 1 .

32 . The fruit of claim 31 , wherein the QTL is homozygously present.

33 . A food product or a processed food product comprising the pepper fruit or a part thereof of claim 27 .

33 . A food product or a processed food product comprising the pepper fruit or a part thereof of claim 29 .

33 . A food product or a processed food product comprising the pepper fruit or a part thereof of claim 31 .

34 . A nucleic acid or a part thereof, optionally in isolated form, which causes a novel flavor in a pepper fruit, which nucleic acid originates from LG3 of a Capsicum baccatum plant or the plant of claim 1 , and is linked thereon to at least one of the molecular markers selected from the group of SEQ ID No:1, SEQ ID No:3, SEQ ID No:5, SEQ ID No:7, SEQ ID No:9 and SEQ ID No:11.

Assignees

• Rijk Zwaan Zaadteelt En Zaadhandel Bv

Inventors

• Eggink Pieter Martijn
• Haanstra Jacob Pieter Willem
• Gutteling Evert Willem
• Bovy Arnaud Guillaume
• Tikunov Yury

Classifications

• C12Q1/6827

  for detection of mutation or polymorphism · CPC title

• C12Q1/6895

  for plants, fungi or algae · CPC title

• A23L19/00

  Products from fruits or vegetables; Preparation or treatment thereof (marmalades, jams, jellies or the like A23L21/10; treating harvested fruit or vegetables in bulk A23N) · CPC title

• C12Q2600/13

  Plant traits · CPC title

• C12Q2600/156

  Polymorphic or mutational markers · CPC title