Articles comprising large-surface-area bio-compatible materials and methods for making and using them

Patent metadata

Field	Value
Publication number	US-2016158412-A1
Application number	US-201514841579-A
Country	US
Kind code	A1
Filing date	Aug 31, 2015
Priority date	Jun 23, 2006
Publication date	Jun 9, 2016
Grant date	—

Abstract

The present invention provides articles of manufacture comprising biocompatible nanostructures comprising significantly increased surface area for, e.g., organ, tissue and/or cell growth, e.g., for bone, tooth, kidney or liver growth, and uses thereof, e.g., for in vitro testing of drugs, chemicals or toxins, or as in vivo implants, including their use in making and using artificial tissues and organs, and related, diagnostic, screening, research and development and therapeutic uses, e.g., as drug delivery devices. The present invention provides biocompatible nanostructures with significantly increased surface area, such as with nanotube and nanopore array on the surface of metallic, ceramic, or polymer materials for enhanced cell and bone growth, for in vitro and in vivo testing, cleansing reaction, implants and therapeutics. The present invention provides optically transparent or translucent cell-culturing substrates. The present invention provides biocompatible and cell-growth-enhancing culture substrates comprising elastically compliant protruding nanostructure substrates coated with Ti, TiO 2 or related metal and metal oxide films.

First claim

1 - 58 . (canceled)

59 : A product of manufacture, comprising:
a substrate; and a layer coupled to, attached to or layered into the substrate, wherein the layer comprises a biocompatible material, and the layer comprises a plurality of nanotubes that: (a) each have a diameter in the range of between about 30 to 600 nm, about 20 to 500 nm, or at least about 150 nm, 200 nm, or 400 nm; (b) the height of the nanotubes is in the range of between about 100 to 10,000 nm; or (c) both (a) and (b).

60 : The product of manufacture of claim 59 , wherein the plurality of nanotubes are vertically aligned and laterally spaced.

61 : The product of manufacture of claim 60 , wherein the biocompatible material is a biocompatible surface layer comprising any of a metal, a metal alloy, titanium, a titanium alloy, a ceramic, titanium oxide, titanium dioxide, aluminum oxide, and any combination thereof.

62 : The product of manufacture of claim 60 , wherein the substrate is a non-metallic substrate comprising any of a polymer, a thermosetting polymer, a porous polymer, a plastic material, an elastomeric polymer, a transparent thermoplastic polymer, a transparent UV-light-curable polymer, a transparent glass, a polydimethylsiloxane (PDMS), a polymethyl methacrylate (PMMA), polyethene, polypropene, polystyrene, poly vinyl chloride, and equivalent compounds and any combination thereof.

63 : The product of manufacture of claim 60 , wherein the layer is coupled to the substrate by applying the layer onto a surface of the substrate using any of a thin-film and a thick-film coating followed by an anodization process.

64 : The product of manufacture of claim 63 , wherein the applying further comprises depositing the layer onto the surface.

65 : The product of manufacture of claim 63 , wherein the anodization process comprises an electrochemical anodization process followed by a heat treatment.

66 : The product of manufacture of claim 65 , wherein the heat treatment crystallizes the plurality of nanotubes.

67 : The product of manufacture of claim 60 , wherein the plurality of nanotubes significantly increase surface area thereby enhancing cell and bone growth, for in vitro and in vivo testing, cleansing reactions, implants, therapeutics.

68 : A dental implant, spinal implant, and orthopedic implant comprising or manufactured to have contained therein the product of manufacture of claim 1 .

69 : The product of manufacture of claim 60 , wherein the plurality of nanotubes comprises any of loose configured Ti short-fibers, particles with high-density surface nanostructure of TiO2 nanotubes, and particles with high-density surface nanostructure of TiO2-covered nanopores or nanowires.

70 : The product of manufacture of claim 60 , wherein the layer includes a micro-nano or macro-nano combined structure in addition to the plurality of nanotubes.

71 : The product of manufacture of claim 60 , wherein the layer comprises, has contained within or is enhanced with any of growth factors, biological agents, antibiotics, genes, proteins, drugs, and magnetic nanoparticles incorporated in, around, and on top of the plurality of nanotubes to provide accelerated and healthy cell growth and drug release for therapeutic use.

72 : The product of manufacture of claim 60 , wherein the plurality of nanotubes comprise any of Ti, Ti oxide, Zr, Hf, Nb, Ta, Mo, W, their alloys, their oxides, and any combination thereof.

73 : The product of manufacture of claim 60 , wherein the layer has a thickness of at least 5 nanometers (nm), or between about 5 to 100 nm.

74 : The product of manufacture of claim 60 , wherein the layer has a coating coverage of at least 50% of the substrate.

75 : The product of manufacture of claim 60 , wherein the plurality of nanotubes comprise an elastically compliant nanostructure substrate, optionally coated with TiO 2 .

76 : The product of manufacture of claim 60 , wherein the substrate is a radiotranslucent thermoplastic polymer and the layer is a thin film nanostructure.

77 : A method, comprising:
providing a substrate; and coupling a layer to the substrate, wherein the layer comprises a biocompatible material, and the layer comprises a plurality of nanotubes that: (a) each have a diameter in the range of between about 30 to 600 nm, about 20 to 500 nm, or at least about 150 nm, 200 nm, or 400 nm; (b) the height of the nanotubes is in the range of between about 100 to 10,000 nm; or (c) both (a) and (b).

Assignees

• Univ California

Inventors

• Jin Sungho
• Oh Seunghan

Classifications

• A61L27/30 Primary

  Inorganic materials · CPC title

• A61L27/54

  Biologically active materials, e.g. therapeutic substances {(A61L27/227 takes precedence)} · CPC title

• C23C28/32

  including at least one pure metallic layer · CPC title

• C23C28/345

  with at least one oxide layer · CPC title

• A61L27/14

  Macromolecular materials · CPC title