Nanofibrous materials as drug, protein, or genetic release vehicles

Patent metadata

Field	Value
Publication number	US-2016158160-A1
Application number	US-201615046675-A
Country	US
Kind code	A1
Filing date	Feb 18, 2016
Priority date	Mar 4, 2005
Publication date	Jun 9, 2016
Grant date	—

Abstract

The present invention is a bioactive, nanofibrous material construct which is manufactured using a unique electrospinning perfusion methodology. One embodiment provides a nanofibrous biocomposite material formed as a discrete textile fabric from a prepared liquid admixture of (i) a non-biodegradable durable synthetic polymer; (ii) a biologically active agent; and (iii) a liquid organic carrier. These biologically-active agents are chemical compounds which retain their recognized biological activity both before and after becoming non-permanently bound to the formed textile material; and will become subsequently released in-situ as discrete freely mobile agents front the fabric upon uptake of water from the ambient environment.

First claim

What is claimed is:

1 . A method of forming a fabricated textile by electrospinning perfusion, the method comprising steps of:
dissolving a non-biodegradable polymer in a solvent to provide an admixture, the dissolving occurring at an ice-cold temperature; permitting the admixture to warm to a temperature between about 20° C. and about 50° C.; loading the admixture into an electrospinning perfusion instrument which can be set at a specified flow rate; perfusing the admixture onto a target surface at the specified flow rate, the step of perfusing occurring at a temperature between about 20° C. and about SOX to provide a perfused material; and permitting trace solvent to be removed from the perfused material to form a fabricated textile.

2 . The method of claim 1 , further comprising dissolving at least one biologically active agent such that the admixture comprises a mixture of the non-biodegradable polymer and the at least one biologically active agent.

3 . The method of claim 1 , wherein the step of permitting trace solvent to be removed is performed using a post-treatment process performed after the step of perfusing the admixture.

4 . The method of claim 1 , wherein the target surface is a mandrel.

5 . The method of claim 1 , wherein the target surface is a stent.

6 . The method of claim 1 , wherein the fabricated textile has a single-strand yarn shape having a diameter between about 0.025 mm and about 2 mm.

7 . The method of claim 1 , wherein the non-biodegradable polymer is selected from the group consisting of a non-biodegradable polyester, a polyurethane, and combinations thereof.

8 . The method of claim 1 , wherein the target surface comprises a first portion and a second portion and the step of perfusing the admixture onto the target surface perfuses the admixture for a first period of time onto the first portion and for a second period of time onto the second portion, wherein the first period and the second period are different.

9 . The method of claim 1 , the method further comprising removing the perfused fabricated textile from the target surface.

10 . The method of claim 9 , wherein the perfused fabricated textile tot formed into a tubular construct.

11 . The method of claim 10 , wherein the tubular construct has an internal diameter of at least 1 mm and less than 40 mm.

12 . The method of claim 10 , wherein the tubular construct has a length of at least about 1 cm and less than about 80 cm.

13 . The method of claim 9 , wherein the perfused fabricated textile is formed into a rounded cuff shape.

14 . The method of claim 9 , wherein the perfused fabricated textile is a flat sheet with a width of at least 1 cm and a length of at least 1 cm.

15 . The method of claim 14 , further comprising fusing the perfused fabricated textile to a second flat sheet comprising a biodegradable polymer.

16 . The method of claim 1 , wherein the step of dissolving further comprises dissolving a biodegradable polymer such that the admixture comprises a mixture of the non-biodegradable polymer and the biodegradable polymer.

17 . The method of claim 2 , wherein the at least one biologically-active agent is maintained at a temperature below about 50° C. during the steps of dissolving, loading, perfusing and permitting such that the at least one biologically active agent maintains the same biological activity after the method as the at least one biologically active agent had before the method.

18 . A fabricated textile suitable for use as a medical article, the fabricated textile being formed by an electrospinning perfusion method comprising:
dissolving a non-biodegradable polymer in a solvent to provide an admixture, the dissolving step occurring at an ice-cold temperature; permitting the admixture to warm to a temperature between about 20° C. and about 50° C.; loading the admixture into an electrospinning perfusion instrument which can be set at a specified flow rate; perfusing the admixture onto a target surface at the specified flow rate, the step of perfusing occurring at a temperature between about 20° C. and about 50° C. to provide a perfused material; and permitting trace solvent to be removed from the perfused material to form a fabricated textile.

19 . The fabricated textile of claim 18 , wherein the method further comprises dissolving at least one biologically active agent such that the admixture comprises a mixture of the non-biodegradable polymer and the at least one biologically active agent.

20 . The fabricated textile of claim 18 , wherein the non-biodegradable polymer is selected from the group consisting of a non-biodegradable polyester, a polyurethane, and combinations thereof.

Assignees

• Biosurfaces Inc
• Univ Clemson
• Rhode Island Board Of Education

Inventors

• Phaneuf Matthew D
• Brown Philip J
• Bide Martin J

Classifications

• D04H3/02

  characterised by the method of forming fleeces or layers, e.g. reorientation of yarns or filaments · CPC title

• A61K31/4196

  1,2,4-Triazoles · CPC title

• A61K31/496 Primary

  Non-condensed piperazines containing further heterocyclic rings, e.g. rifampin, thiothixene or sparfloxacin · CPC title

• D04H1/728

  by electro-spinning {(electro-spinning methods and apparatus D01D5/0007)} · CPC title

• D04H1/435

  Polyesters · CPC title