Reconfigurable assembly with faraday wave-based templates

Patent metadata

Field	Value
Publication number	US-2016145566-A1
Application number	US-201414898297-A
Country	US
Kind code	A1
Filing date	Jun 20, 2014
Priority date	Jun 21, 2013
Publication date	May 26, 2016
Grant date	—

Abstract

A method manufactures a structure based on reconfigurable assembly with faraday wave-based templates. The method includes the steps of providing a chamber containing a gas-liquid interface or liquid-liquid interface and dispersing a plurality of floaters at the gas-liquid interface or liquid-liquid interface. The method further includes oscillating the chamber along an axis orthogonal to the gas-liquid interface or liquid-liquid interface, thereby generating a standing wave formed by a parametric instability on the surface of the liquid. After formation of the standing wave, the floaters are allowed to self-assemble, at which point the floaters can be linked together.

First claim

What is claimed is:

1 . A method of manufacturing a structure, comprising:
providing a chamber containing a gas-liquid interface or liquid-liquid interface; dispersing a plurality of floaters at the gas-liquid interface or liquid-liquid interface; oscillating the chamber along an axis orthogonal to the gas-liquid interface or liquid-liquid interface, thereby generating a standing wave at the gas-liquid interface or liquid-liquid interface; allowing the floaters to self-assemble; and linking the floaters, wherein the standing wave is formed by a parametric instability on the surface of the liquid.

2 . The method of claim 1 , wherein the standing wave is a Faraday wave.

3 . The method of claim 1 , wherein the floaters have a diameter of about 0.1 μm to about 1 m.

4 . The method of claim 1 , wherein the floaters have a diameter of about 10 μm to about 5 mm.

5 . The method of claim 1 , wherein the floaters are at least one of a biological sample, a chemical sample and a non-biomaterial unit,
wherein the biological sample is at least one of microorganisms, cells, cell clusters, cell spheroids, cell fragments, viruses, bacteria, fungi, peptides, nucleic acids, proteins, carbohydrates, secreted cellular products and exosomes, wherein the chemical sample is at least one of biomaterial units, hydrogel units and polymer units, and wherein the non-biomaterial unit is at least one of semiconductor units and metallic units.

6 . The method of claim 5 , wherein at least a portion of the floaters encapsulate or are coated with the biological sample.

7 . The method of claim 6 , wherein the biological sample is a microorganism, a cell, a cell cluster, a cell spheroid, a cell fragment, a virus, a bacteria, a fungi, a peptide, a nucleic acid, a protein, a carbohydrate, a secreted cellular product, or an exosome.

8 . The method of claim 1 , wherein the step of linking the plurality of floaters further comprises photo cross-linking , UV cross-linking, chemical cross-linking , thermo cross-linking, surface molecule recognition-based linking, or geometric shape-based linking.

9 . The method of claim 8 , further comprising:
forming a monolayer structure following the step of linking the plurality of floaters; repeating the method of claim 8 to produce a plurality of monolayer structures; and stacking the monolayer structures layer by layer into a 3D architecture.

10 . The method of claim 9 , further comprising:
culturing the 3D architecture, thereby forming 3D tissue constructs.

11 . The method of claim 1 , further comprising:
forming a monolayer structure following the step of linking the plurality of floaters.

12 . A structure made by the method of claim 1 .

13 . A system for manufacturing a structure, comprising:
a chamber having a bottom surface; a liquid disposed in the chamber; a plurality of floaters disposed on the liquid; an oscillating mechanism configured to oscillate the chamber along an axis orthogonal to the gas-liquid interface or liquid-liquid interface, thereby generating a standing wave at the gas-liquid interface or liquid-liquid interface; and a linking mechanism configured to link the plurality of floaters; wherein the standing wave is formed by a parametric instability on the surface of the liquid.

14 . The system of claim 13 , wherein the standing wave is a Faraday wave.

15 . The system of claim 13 , wherein the plurality of floaters have a diameter of about 10 μm to about 5 mm.

16 . The system of claim 13 , wherein the floaters are at least one of a biological sample, a chemical sample and a non-biomaterial unit,
wherein the biological sample is at least one of microorganisms, cells, cell clusters, cell spheroids, cell fragments, viruses, bacteria, fungi, peptides, nucleic acids, proteins, carbohydrates, secreted cellular products and exosomes, wherein the chemical sample is at least one of biomaterial units, hydrogel units and polymer units, and wherein the non-biomaterial unit is at least one of semiconductor units and metallic units.

17 . The system of claim 16 , wherein at least a portion of the floaters encapsulate the biological sample.

18 . The system of claim 17 , wherein the biological sample is a microorganism, a cell, a cell cluster, a cell spheroid, a cell fragment, a virus, a bacteria, a fungi, a peptide, a nucleic acid, a protein, a carbohydrate, a secreted cellular product, or an exosome.

19 . The system of claim 13 , further comprising:
a substrate for stacking a plurality of monolayer structures layer by layer into a 3D architecture.

20 . The system of claim 19 , further comprising:
a culture chamber for culturing the 3D architecture into 3D tissue constructs.

21 . A method of manufacturing a structure, comprising:
providing a chamber containing a gas-liquid interface or liquid-liquid interface; dispersing a plurality of floaters at the gas-liquid interface or liquid-liquid interface, the floaters having a diameter of about 10 μm to about 5 mm; oscillating the chamber along an axis orthogonal to the gas-liquid interface or liquid-liquid interface, thereby generating a Faraday wave at the gas-liquid interface or liquid-liquid interface, the Faraday wave formed by a parametric instability on the surface of the liquid; allowing the floaters to self-assemble; and linking the floaters to form the structure.

Assignees

• Brigham & Womens Hospital

Inventors

• Chen Pu
• Demirci Utkan

Classifications

• B01D43/00 Primary

  Separating particles from liquids, or liquids from solids, otherwise than by sedimentation or filtration (flotation processes B03D1/00; drying solid materials or objects F26B) · CPC title

• C12N5/0062 Primary

  General methods for three-dimensional culture · CPC title

• C12N13/00

  Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves · CPC title

• C12M21/08

  for producing artificial tissue or for ex-vivo cultivation of tissue (prostheses A61F2/00, grafts A61L27/00) · CPC title

• C12M35/04

  Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli · CPC title