Plate, method for manufacturing plate, method for observing biochip, and method for screening

Patent metadata

Field	Value
Publication number	US-2016144368-A1
Application number	US-201615010652-A
Country	US
Kind code	A1
Filing date	Jan 29, 2016
Priority date	Aug 2, 2013
Publication date	May 26, 2016
Grant date	—

Abstract

This plate ( 100 ) is provided with a board ( 130 ) having a first surface, and partitioned member ( 120 ) in which a plurality of partitions are formed by dividing walls ( 22 ). Gaps ( 28 ) are formed to the first surface side between neighboring dividing walls ( 22 ). The ends of the dividing walls ( 22 ) at the gap ( 28 ) side have welded portions welded to the first surface of the board ( 130 ), the board ( 130 ) and the partitioned member ( 120 ) being welded at the welded portions.

First claim

1 . A plate, comprising:
a board having a first surface; and a partitioned member that forms a plurality of partitions using barrier walls, wherein a gap, which is a space between adjacent barrier walls, is formed on the first surface side, an end on the gap side of the barrier wall has a welded part that joins with the first surface of the board, and the board and the partition member are welded together at the welded part.

2 . The plate according to claim 1 , wherein the board and partitioned member above are joined together using ultrasonic welding.

3 . The plate according to claim 1 , wherein the welded part is welded to the first surface of the board across the entire circumference of the barrier wall.

4 . The plate according to claim 1 , wherein the welded part is welded to the first surface of the board on a portion of the circumference of the barrier wall.

5 . The plate according to claim 4 , wherein the welded part has a protruding shape, and a sealing member is provided arranged between the partitioned member and the board.

6 . The plate according to claim 5 , wherein the protruding welded part passes through the sealing member.

7 . The plate according to claim 1 wherein the board and the partitioned member are formed using the same optically transparent resin material.

8 . The plate according to claim 1 , wherein a protective film that covers the open surface of the partition is provided.

9 . The plate according to claim 1 , wherein a biochip is provided that is arranged in the bottom of the partition in the first surface.

10 . A method for manufacturing a plate having a plurality of wells formed by joining a partitioned member in a first surface of a board, wherein a barrier wall of the partitioned member that forms the wells has a gap, which is a space between adjacent barrier walls, on the first surface side of the board, comprising:
a welding step that welds the first surface of the board and the partitioned member together at a welded part provided on an end on the gap side of the barrier wall.

11 . The method for manufacturing a plate according to claim 10 , wherein, in the welding step, the welded part of the partitioned member and the first surface of the board are brought into contact and joined by heating past a melting point and then applying pressure.

12 . The method for manufacturing a plate according to claim 10 , wherein, in the welding step, the welded part of the partitioned member and the first surface of the board are ultrasonically welded together.

13 . The method for manufacturing a plate according to claim 10 , wherein a sealing member having a shape that follows the shape of an end on the gap side of the barrier wall on the first surface of the board is provided, comprising:
a step that aligns the positions of the sealing member and the partitioned member.

14 . The method for manufacturing a plate according to claim 10 , wherein, in the welding step, the welded part is welded across the entire circumference of the barrier wall against the first surface of the board.

15 . The method for manufacturing a plate according to claim 10 , wherein, in the welding step, the welded part is welded on a portion of the circumference of the barrier wall against the first surface of the board.

16 . The method for manufacturing a plate according to claim 13 , comprising:
an inserting step that inserts the welded part into the sealing member and then brings the first surface of the board and the welded part of the partitioned member into contact.

17 . A method for observing a biochip, comprising:
a removing step that removes the partitioned member from the plate; and an observing step for observing a biochip arranged on the first surface of the board.

18 . A screening method that uses a plate having a biochip, comprising:
a dispensing step for dispensing a specimen containing a target that is able to react specifically to a biomolecule fixed on the biochip in the plate according to claim 9 ; a cleaning step for cleaning the plate; a drying step for drying the plate; and a detecting step for detecting affinity between the target and the biomolecule.

19 . A plate, comprising:
a board; and a regulating member that is joined to the board through welding and regulates a support area in order to support the biochip.

20 . A plate, in the plate according to claim 19 , comprising:
a first regulating member that regulates a first support area in order to support a first biochip where the regulating member is joined to the board through welding; and a second regulating member that regulates a second support area in order to support a second biochip, that is a member configured with a body that is separate from the first regulating member, and is joined to the board through welding.

21 . A plate, in the plate according to claim 20 , wherein the length of the first regulating member and the length of the second regulating member are the same in a direction perpendicular to the surface where the board and the regulating members join.

22 . A plate, in the plate according to claim 19 ,
wherein the regulating member has a gap on the end that joins with the board.

23 . A plate, in the plate according to claim 19 ,
wherein the support areas regulated by the regulating members are round.

24 . A plate, in the plate according to claim 19 ,
wherein the board is formed using a material that is translucent.

25 . A plate, in the plate according to claim 19 ,
wherein the regulating members are formed using a material that is translucent.

26 . A plate, in the plate according to claim 19 ,
wherein the board is formed from the same material as the regulating members.

27 . A plate, in the plate according to claim 19 ,
wherein the regulating members are joined to the board using ultrasonic welding.

Assignees

• Nikon Corp

Inventors

• Isami Tadao
• Hamashima Muneki
• Ueda Takehito
• Hayashi Yutaka

Classifications

• B01L2300/044

  pierceable, e.g. films, membranes · CPC title

• B01L2300/168

  Specific optical properties, e.g. reflective coatings · CPC title

• B29C66/112

  Single lapped joints · CPC title

• B29C66/5346

  said single elements being substantially flat · CPC title

• B01L2300/0829

  Multi-well plates; Microtitration plates · CPC title