Peptide and Complex of Same, Scaffold for Tissue Repair and Surface Treatment Method for Same, and Surface Treatment Solution or Set of Treatment Solutions

Patent metadata

Field	Value
Publication number	US-2016137694-A1
Application number	US-201414787228-A
Country	US
Kind code	A1
Filing date	Apr 25, 2014
Priority date	Apr 26, 2013
Publication date	May 19, 2016
Grant date	—

Abstract

Provided are a peptide that enables surface treatment of a scaffold for tissue repair that makes it possible to accelerate the repair of living tissue without using a material that negatively affects the repair of living tissue, a complex containing this peptide, a scaffold for tissue repair surface treated using this peptide or this complex, a surface treatment method for a scaffold for tissue repair using this peptide or this complex, and a treatment solution or set of treatment solutions to be used in this surface treatment method. Surface treatment of a scaffold for tissue repair is conducted by combining glycosaminoglycan and a peptide containing adhesive sites and basic sites each comprising predetermined amino acid residues.

First claim

1 . A peptide comprising, in the amino acid sequence thereof, an adhesive site consisting of residues of amino acids including a 2,3-dihydroxyphenyl group or 3,4-dihydroxyphenyl group, and a basic site including three or more consecutive basic amino acid residues.

2 . The peptide according to claim 1 , wherein the adhesive site and the basic site bind via a spacer site.

3 . The peptide according to claim 1 , wherein N terminus or C terminus is a basic terminus that includes a basic site at the terminus or vicinity thereof, and
wherein a site in which an amino acid residue is not present, or consisting of at least 1 to no more than 5 amino acid residues and not including the adhesive site is present on a basic terminus side from the basic site.

4 . The peptide according to claim 3 , wherein another end relative to the basic terminus is an adhesive terminus having an adhesive site at a terminus or vicinity thereof, and
wherein the adhesive site is contained and the basic site is not contained within five amino acid residues from the adhesive terminus.

5 . The peptide according to claim 4 , wherein a sequence of nine resides from the C terminus is an amino acid sequence represented by SEQ ID NO 1.

6 . A complex comprising the peptide according to claim 1 , and glycosaminoglycan.

7 . A complex of the peptide according to claim 1 , glycosaminoglycan and cell growth factor,
wherein the cell growth factor is binding with the glycosaminoglycan, and binding with the adhesive site of the peptide via the glycosaminoglycan.

8 . A scaffold for tissue repair, wherein the peptide according to claim 1 is binding to the surface thereof at the adhesive site.

9 . A scaffold for tissue repair having glycosaminoglycan on a surface thereof,
wherein the peptide according to claim 1 is binding with the surface of the scaffold at the adhesive site, and wherein the glycosaminoglycan is binding with the basic site of the peptide.

10 . A scaffold for tissue repair having cell growth factor on a surface thereof,
wherein the peptide according to claim 1 is binding with the surface of the scaffold at the adhesive site, and wherein the cell growth factor is binding with glycosaminoglycan, and binding with the basic site of the peptide via the glycosaminoglycan.

11 . (canceled)

12 . (canceled)

13 . (canceled)

14 . (canceled)

15 . (canceled)

16 . (canceled)

17 . A treatment solution for surface treatment of a scaffold for tissue repair, comprising the peptide according to claim 1 .

18 . A set of treatment solutions for surface treatment of a scaffold for tissue repair, comprising the treatment solution according to claim 17 and a treatment solution containing glycosaminoglycan.

19 . The set of treatment solutions for surface treatment of a scaffold for tissue repair according to claim 18 , further comprising a treatment solution containing cell growth factor.

20 . A treatment solution for surface treatment of a scaffold for tissue repair, comprising the complex according to claim 6 .

21 . A set of treatment solutions for surface treatment of a scaffold for tissue repair, comprising the treatment solution according to claim 20 and a treatment solution containing cell growth factor.

22 . A treatment solution for surface treatment of a scaffold for tissue repair, comprising the complex according to claim 7 .

Assignees

• Nat Cerebral & Cardiovascular Ct
• Jms Co Ltd

Inventors

• Yamaoka Tetsuji
• Kakinoki Sachiro
• Baba Shunsuke
• Hashimoto Yoshiya

Classifications

• A61L2300/236

  Glycosaminoglycans, e.g. heparin, hyaluronic acid, chondroitin · CPC title

• A61L2300/414

  Growth factors · CPC title

• A61L2300/25

  Peptides having up to 20 amino acids in a defined sequence · CPC title

• A61L27/54 Primary

  Biologically active materials, e.g. therapeutic substances {(A61L27/227 takes precedence)} · CPC title

• C07K9/00

  Peptides having up to 20 amino acids, containing saccharide radicals and having a fully defined sequence; Derivatives thereof · CPC title