Alpha-amylase variants
US-2015376588-A1 · Dec 31, 2015 · US
US2016135472A1 · US · A1
| Field | Value |
|---|---|
| Publication number | US-2016135472-A1 |
| Application number | US-201414781164-A |
| Country | US |
| Kind code | A1 |
| Filing date | Apr 2, 2014 |
| Priority date | Apr 5, 2013 |
| Publication date | May 19, 2016 |
| Grant date | — |
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The present invention provides a method of producing a baked product having reduced crumb elasticity, said method comprising adding a raw starch degrading alpha-amylase, a lipase and a phospholipase to dough ingredients comprising flour, water, and yeast and mixing to prepare a dough; leavening the dough; and baking the dough to obtain the baked product.
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1 . A method of producing a baked product having reduced crumb elasticity, said method comprising a) adding a raw starch degrading alpha-amylase, a lipase and a phospholipase to dough ingredients comprising flour, water, and yeast and mixing to prepare a dough; b) leavening the dough, and c) baking the dough. 2 . The method according to claim 1 , wherein the raw starch degrading alpha-amylase has an amino acid sequence having at least 70% identity to SEQ ID NO:1. 3 . The method according to claim 1 , wherein step a) further comprises adding an anti-staling amylase. 4 . The method according to claim 1 , wherein the anti-staling amylase is a maltogenic alpha-amylase having at least 70% identity to SEQ ID NO:2. 5 . The method according to claim 1 , wherein step a) further comprises adding one or more additional enzymes selected from the group consisting of a xylanase, a galactolipase, a protease, a transglutaminase, a cellulase, a hemicellulase, an acyltransferase, a protein disulfide isomerase, a pectinase, a pectate lyase, an oxidoreductase, a peroxidase, a laccase, a glucose oxidase, a pyranose oxidase, a hexose oxidase, a lipoxygenase, an L-amino acid oxidase or a carbohydrate oxidase, and/or a sulfurhydryl oxidase, a non-raw starch degrading alpha-amylase, and a glucoamylase. 6 . A baked product obtainable by the method according to claim 1 . 7 . A dough prepared by adding a raw starch degrading alpha-amylase, a lipase and a phospholipase to dough ingredients comprising flour, water, and yeast, and mixing. 8 . A baking composition comprising a raw starch degrading alpha-amylase, a lipase and a phospholipase. 9 . The baking composition of claim 8 , wherein the composition additionally comprises an anti-staling amylase. 10 . The baking composition of claim 8 , which is a dough, a flour composition, or a flour pre-mix. 11 . The baking composition of claim 8 , which is in the form of a granulate or agglomerated powder. 12 . The baking composition of claim 8 , wherein 95% (by weight) of the granulate or agglomerated powder has a particle size between 25 and 500 μm. 13 . The baking composition according to claim 8 , wherein the raw starch degrading alpha-amylase has an amino acid sequence having at least 70% identity to SEQ ID NO:1. 14 . (canceled) 15 . A method of producing a baked product having reduced crumb elasticity, said method comprising adding an anti-staling amylase and a raw starch degrading alpha-amylase to dough ingredients comprising flour, water, and yeast and mixing to prepare a dough; leavening the dough; and baking the dough to obtain the baked product.
Solid, dry or compact materials; Granules; Powders · CPC title
with enzymes · CPC title
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