Actinomycete integrative and conjugative element from actinoplanes sp. se50/110 as plasmid for genetic transformation of related actinobacteria

Patent metadata

Field	Value
Publication number	US-2016130622-A1
Application number	US-201514939495-A
Country	US
Kind code	A1
Filing date	Nov 12, 2015
Priority date	Dec 8, 2011
Publication date	May 12, 2016
Grant date	—

Abstract

The present invention is directed to an innate DNA sequence within the complete genome sequence of Actinoplanes sp. SE50/110 which resembles the structure of an actinomycete integrative and conjugative element (AICE). Related AICEs were used for establishing genetic manipulation tools for other bacteria in the past. In this document, we describe the unique features of the specific AICE found in Actinoplanes sp. SE50/110 which are clearly distinct from any other known AICE as a whole, but share minor parts with varying sequence similarity with other characterized AICEs from other species.

First claim

1 . An actinomycete integrative and conjugative element being selected from the group consisting of:
a polynucleotide having the sequence of SEQ ID 1, a polynucleotide which hybridizes under stringent conditions to a polynucleotide as specified in (a) and a polynucleotide having at least 90% identity with the sequence of SEQ ID 1.

2 . The actinomycete integrative and conjugative element of claim 1 having at least 95% identity with the sequence of SEQ ID 1.

3 . A host cell transformed with the actinomycete integrative and conjugative element of claim 1 .

4 . The host cell of claim 3 wherein the host cell is an Actinoplanes sp.

5 . A method for preparation of biological products comprising the steps of
culturing the host cell of claim 3 in a useful medium, harvesting the product from the culture and isolating and purifying the product.

6 . The method of claim 5 wherein the product is acarbose.

Assignees

• Bayer Ip Gmbh

Inventors

• Klein Andreas
• Selber Klaus
• Wehlmann Hermann
• Rosen Winfried
• Pühler Alfred
• Schwientek Patrick
• Kalinowski Jörn
• Wehmeier Udo

Classifications

• C12P19/26 Primary

  Preparation of nitrogen-containing carbohydrates · CPC title

• C12N15/76

  for Actinomyces; for Streptomyces · CPC title

• C12N15/74

  Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora · CPC title

• C12P19/28

  N-glycosides · CPC title

• C12N15/52

  Genes encoding for enzymes or proenzymes · CPC title