Thermal reaction device and method for using the same

Patent metadata

Field	Value
Publication number	US-2016129441-A1
Application number	US-201514873958-A
Country	US
Kind code	A1
Filing date	Oct 2, 2015
Priority date	Apr 3, 2003
Publication date	May 12, 2016
Grant date	—

Abstract

An M×N matrix microfluidic device for performing a matrix of reactions, the device having a plurality of reaction cells in communication with one of either a sample inlet or a reagent inlet through a via formed within an elastomeric block of the device. Methods provided include a method for forming vias in parallel in an elastomeric layer of an elastomeric block of a microfluidic device, the method comprising using patterned photoresist masks and etching reagents to etch away regions or portions of an elastomeric layer of the elastomeric block.

First claim

1 . (canceled)

2 . A high density microfluidic device comprising
a first layer containing flow channels and a second layer containing flow channels; a first flow channel in the first layer, said first flow channel in fluidic communication with a first inlet; a second flow channel in the second layer, said second flow channel in fluidic communication with the first flow channel; a third flow channel in the second layer, said third flow channel in fluidic communication with a second inlet; wherein the second and third flow channels both cross over the first flow channel; and wherein the second and third flow channels are in fluidic communication through a fourth flow channel in the second layer.

3 . The microfluidic device of claim 2 , wherein the second flow channel and the third flow channel are about parallel to each other and are substantially perpendicular to the first.

4 . The microfluidic device of claim 2 , wherein the microfluidic device comprises a plurality of first, second, third, and fourth flow channels and each first flow channel is in fluid communication with the plurality of second flow channels.

5 . The microfluidic device of claim 2 , wherein the first and second layers comprise an elastomeric material.

6 . The microfluidic device of claim 2 , wherein at least one of the flow channels is partitioned into a plurality of separate chambers by one or more valves, said valves being formed in said microfluidic device such that a deflectable membrane(s) can be deflected into said sample channel to partition said sample channel into one or more separate chambers.

Assignees

• Fluidigm Corp

Inventors

• Mcbride Lincoln
• Facer Geoffrey
• Unger Marc
• Lucero Michael
• Nassef Hany Ramez

Classifications

• B01L2300/0864

  comprising only one inlet and multiple receiving wells, e.g. for separation, splitting · CPC title

• B01L2400/0481

  squeezing of channels or chambers · CPC title

• G01N27/453

  Cells therefor · CPC title

• B01L3/502715 Primary

  characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces · CPC title

• C12Q1/686

  Polymerase chain reaction [PCR] · CPC title