Three-dimensional optical coherence tomography apparatus and its application

Patent metadata

Field	Value
Publication number	US-2016124202-A1
Application number	US-201514816172-A
Country	US
Kind code	A1
Filing date	Aug 3, 2015
Priority date	Nov 5, 2014
Publication date	May 5, 2016
Grant date	—

Abstract

Provided herein are devices and systems that apply full-field optical coherence tomography (OCT) technology to three-dimensional skin tissue imaging. A special designed Mirau type objective and an optical microscope module allowing both OCT mode and orthogonal polarization spectral imaging (OPSI) mode are disclosed.

First claim

1 . A device comprising a light source module configured to provide a source light to an optical microscope module, which handles the source light and processes light signal; a Mirau type objective module, which handles light from the optical microscope module and process light signal generated from a tissue translation module holding a tissue sample; and a data processing unit for analyzing light signals from the tissue sample, wherein said Mirau type objective module comprises an interference objective immersed in a media, and wherein said optical microscope module comprises an optical switch configured to toggle between optical coherence tomography (OCT) mode and orthogonal polarization spectral imaging (OPSI) mode.

2 . The device of claim 1 , wherein the light source module comprises an amplified spontaneous emission light source, a superluminescent diode, a light emitting diode (LED), a broadband supercontinuum light source, a mode-locked laser, a tunable laser, a Fourier-domain Mode-locking light source, an optical parametric oscillator (OPO), a halogen lamp, a Ce 3+ :YAG crystal fiber, a Ti 3+ :Al 2 O 3 crystal fiber, or a Cr 4+ :YAG crystal fiber.

3 . The device of claim 2 , wherein the light source module comprises a Ce 3+ :YAG crystal fiber.

4 . The device of claim 1 , wherein said Mirau type objective module comprises an interference objective lens immersed in a media, a first glass plate, a second glass plate in a sealed container filled with one or more media.

5 . The device of claim 1 , wherein the interference objective lens immersed in a media having optical characteristics similar to the tissue sample to be analyzed.

6 . The device of claim 5 , wherein the optical characteristics is refractive index.

7 . The device of claim 6 , wherein the media has a refractive index in a range of about 1.2 to about 1.8.

8 . The device of claim 7 , wherein said media comprises water, silicone oil, or glycerol.

9 . The device of claim 8 , wherein said media comprises silicone oil.

10 . The device of claim 4 , wherein the one or more media comprises a first media and a second media.

11 . The device of claim 1 , wherein said first media comprises water and the second media comprises silicone oil.

12 . The device of claim 1 , wherein the optical switch is a quarter-wave plate where the fast axis of the quarter-wave plate is set to an angle of 45° allowing a portion of a signal light reflected or scattered from the sample passing through with polarization state unchanged.

13 . The device of claim 1 , wherein the optical switch is a quarter-wave plate where the fast axis of the quarter-wave plate is set to an angle of 0° or 90° allowing a portion of a signal light reflected or scattered from the sample passing through with polarization state rotated by 90°.

14 . The device of claim 1 , wherein the optical microscope module further comprises an objective lens, an optical long-wave-pass filter, and a polarization beam splitter.

15 . The device of claim 1 , wherein the tissue translation module comprises a cover glass and a transversely motorized linear stage on a tissue holder means.

16 . The device of claim 1 , wherein the data processing unit comprises a one-dimensional detector, or a two-dimensional detector, optionally coupled a computer, or combinations thereof.

17 . The device of claim 16 , wherein the two-dimensional detector is a charge-coupled device (CCD), a multi-pixel camera, or a complementary metal oxide semiconductor (CMOS) camera, or combination thereof.

18 . A system for imaging a tissue sample comprising a light source module configured to provide a source light to an optical microscope module, which handles the source light and processes light signal; a Mirau type objective module, which handles light from the optical microscope module and process light signal generated from a tissue translation module holding a tissue sample; and a data processing unit for analyzing light signals from the tissue sample, wherein said Mirau type objective module comprises an interference objective immersed in a media, and wherein said optical microscope module comprises an optical switch configured to toggle between optical coherence tomography (OCT) mode and orthogonal polarization spectral imaging (OPSI) mode.

19 . A method for imaging a tissue sample comprising imaging test light in depth emerging from a sample, and imaging a contrast image of absorption from a substructure of the sample, by a device of claim 1 .

20 . The method of claim 19 , wherein the tissue imaging is determined by complete circumferential peripheral and deep margin assessment.

Assignees

• Univ Nat Taiwan

Inventors

• Huang Sheng-Lung
• Ho Tuan-Shu
• Tsai Chien-Chung

Classifications

• G02B21/0056 Primary

  based on optical coherence, e.g. phase-contrast arrangements, interference arrangements · CPC title

• G02B21/0092

  Polarisation microscopes · CPC title

• G02B21/26

  Stages; Adjusting means therefor · CPC title

• G02B21/008

  Details of detection or image processing, including general computer control · CPC title

• G02B21/0032

  Optical details of illumination, e.g. light-sources, pinholes, beam splitters, slits, fibers (G02B21/0036 - G02B21/008; means for illumination of specimens in general G02B21/06) · CPC title