Novel o-phosphoserine export protein and the method of producing o-phosphoserine using the same

Patent metadata

Field	Value
Publication number	US-2016115507-A1
Application number	US-201414890423-A
Country	US
Kind code	A1
Filing date	May 9, 2014
Priority date	May 10, 2013
Publication date	Apr 28, 2016
Grant date	—

Abstract

The present invention relates to a novel isolated polypeptide having the ability to export O-phosphoserine (OPS) that is a precursor of L-cysteine, a vector comprising the polynucleotide, an OPS-producing microorganism having enhanced activity of the polypeptide, a method of producing OPS using the microorganism, and a method for preparing cysteine or its derivatives, which comprises reacting OPS, produced by the above method, with a sulfide in the presence of O-phosphoserine sulfhydrylase (OPSS) or a microorganism that expresses OPSS.

First claim

1 . A method for producing O-phosphoserine (OPS), comprising culturing an O-phosphoserine-producing microorganism having enhanced activity of a polypeptide that has an amino acid sequence of SEQ ID NO: 1 or 2 and has O-phosphoserine exporting activity.

2 . The method according to claim 1 , wherein the O-phosphoserine-producing microorganism further has reduced activity of endogenous phosphoserine phosphatase.

3 . The method according to claim 1 , wherein the O-phosphoserine-producing microorganism further has enhanced activity of phosphoglycerate dehydrogenase or phosphoserine aminotransferase.

4 . An isolated polypeptide having an amino acid sequence of SEQ ID NO: 1 or 2 and having O-phosphoserine (OPS) exporting activity.

5 . A polynucleotide encoding the polypeptide of claim 4 .

6 . A vector comprising the polynucleotide of claim 5 .

7 . An O-phosphoserine-producing microorganism having enhanced activity of a polypeptide that has an amino acid sequence of SEQ ID NO: 1 or 2 and has O-phosphoserine exporting activity.

8 . The microorganism of claim 7 , wherein the microorganism further has reduced activity of endogenous phosphoserine phosphatase.

9 . The microorganism according to claim 7 , wherein the microorganism further has enhanced activity of phosphoglycerate dehydrogenase or phosphoserine aminotransferase.

10 . A method for producing cysteine or its derivatives, comprising the steps of:
a) culturing the microorganism of claim 7 to produce O-phosphoserine (OPS); and b) reacting the O-phosphoserine, produced in step a), with a sulfide in the presence of O-phosphoserine sulfhydrylase (OPSS) or a microorganism that expresses OPSS.

11 . The method according to claim 10 , wherein the sulfide is one or more selected from the group consisting of Na 2 S, NaSH, (NH 4 ) 2 S, H 2 S and Na 2 S 2 O 3 .

12 . A method for producing cysteine or its derivatives, comprising the steps of:
a) culturing the microorganism of claim 8 to produce O-phosphoserine (OPS); and b) reacting the O-phosphoserine, produced in step a), with a sulfide in the presence of O-phosphoserine sulfhydrylase (OPSS) or a microorganism that expresses OPSS.

13 . The method according to claim 12 , wherein the sulfide is one or more selected from the group consisting of Na 2 S, NaSH, (NH 4 ) 2 S, H 2 S and Na 2 S 2 O 3 .

14 . A method for producing cysteine or its derivatives, comprising the steps of:
a) culturing the microorganism of claim 9 to produce O-phosphoserine (OPS); and b) reacting the O-phosphoserine, produced in step a), with a sulfide in the presence of O-phosphoserine sulfhydrylase (OPSS) or a microorganism that expresses OPSS.

15 . The method according to claim 14 , wherein the sulfide is one or more selected from the group consisting of Na 2 S, NaSH, (NH 4 ) 2 S, H 2 S and Na 2 S 2 O 3 .

Assignees

• Cj Cheiljidang Corp
• Cj Cheiljedang Corp

Inventors

• Kim Hye Won
• Kim Sol
• Chang Jin Sook
• Yoo In Hwa

Classifications

• C12P13/12 Primary

  Methionine; Cysteine; Cystine · CPC title

• C12P13/06

  Alanine; Leucine; Isoleucine; Serine; Homoserine · CPC title

• C07K14/245 Primary

  Escherichia (G) · CPC title

• C12N15/11

  DNA or RNA fragments; Modified forms thereof (DNA or RNA not used in recombinant technology, C07H21/00); {Non-coding nucleic acids having a biological activity} · CPC title

• C07K14/195

  from bacteria · CPC title