Two-phase electroextraction from moving phases

Patent metadata

Field	Value
Publication number	US-2016114261-A1
Application number	US-201414898813-A
Country	US
Kind code	A1
Filing date	Jun 19, 2014
Priority date	Jun 19, 2013
Publication date	Apr 28, 2016
Grant date	—

Abstract

The present invention relates to a process for the electro extraction of molecules from a moving fluid donor phase into an acceptor phase, comprising the steps of: providing an electrically conductive donor phase moving at a first flow velocity and in electrically conductive contact with a first electrode, providing an electrically conductive acceptor phase in direct contact and immiscible with the donor phase, in electrically conductive contact with a second electrode; and providing a supporting or confining phase guide pattern to keep a defined interface between donor phase and acceptor phase, and (d) applying an electrical field between the first and the second electrode.

First claim

1 . A process for the electroextraction of compounds from a moving fluid donor phase into an acceptor phase, comprising the steps of:
a) providing an electrically conductive donor phase moving at a first flow velocity and in electrically conductive contact with a first electrode, b) providing an electrically conductive acceptor phase in direct contact and immiscible with the donor phase, in electrically conductive contact with a second electrode; and c) providing a supporting or confining phase guide pattern to keep a defined interface between donor phase and acceptor phase, and (d) applying an electrical field between the first and the second electrode.

2 . A process according to claim 1 , wherein the acceptor phase is moving co-directionally with the donor phase at a second flow velocity lower than the first flow velocity, preferably stagnant.

3 . A process according to any one of the previous claims, wherein the donor and/or acceptor phase remain immiscible during the time scale and under the conditions of the process.

4 . A process according to any one of the previous claims, wherein the electrical field is applied sufficiently high and in a sufficiently long period of time to allow at least part of the analyte compounds to migrate from the donor phase to the acceptor phase, or to the interface between donor and acceptor phase.

5 . A process according to any one of the previous claims, wherein a further acceptor phase is present in at the opposite side of the first acceptor phase and in direct contact with the donor phase, and wherein the first electrode is arranged in the further acceptor phase, and wherein the electrically conductive contact with the donor phase occurs through the further acceptor phase.

6 . A process according to any one of the previous claims, further comprising removing the acceptor phase comprising the migrated analytes, and subjecting the removed acceptor phase to a further separation and/or analysis process.

7 . A process according to any one of the previous claims, wherein the donor phase is the result of a previous separation and/or analysis process.

8 . A process according to claim 6 or claim 7 , wherein the further separation and/or analytical methods include LC such as RP, NP, TLC; CE, NMR, MS and coupled methods such as GC/MS or GC-MS/MS; in particular nanoelectrospray-Direct-Infusion-MS, UV/VIS, nano LC, HPLC, UPLC; RP-(UV/VIS)-EV-NP-MS; RP-(UV/VIS)-EV-NMR; NP-(UV/VIS)-EV-CE-MS/RP-(UV/VIS)-EV-CE-MS; RP(UV/VIS)-EV-TLC/NP-(UV/VIS)-EV-TLC; EV-nano LC and CE-EV.

9 . A device for the two phase electroextraction process according to any one of claims 1 to 8 , comprising:
(i) a channel for transporting a fluid stream comprising a volume defined by a channel floor and two channel walls, and
(ii) a first electrode for contacting a first phase arranged at one of the channel walls, and a second electrode for contacting a second phase arranged at an opposite channel wall to the first electrode to apply an electrical field in orthogonal direction of the flow direction, and means for applying an electrical field over the two electrodes, and
(iii) a contour phase guide arranged on the channel floor and following the contours of the floor at a given distance to the channel walls from the inlet to the outlet conduit, for separating the at least two phases by pinning the meniscus of the phase boundaries to the phase guide.

10 . A device according to claim 9 , wherein the lumen is internally divided into at least a first and a second volume by at least one phase guide formed inside the lumen, further comprising
(iv) at least a first and a second inlet fluid conduit in fluid communication with the channel lumen and the upstream exteriors of the conduit, wherein the first and second inlet conduit are in fluid communication with the first and second volume, respectively, and (iii) at least a first and second outlet conduit in fluid connection with the respective first and second downstream part of the lumen volumes and the exterior of the channel.

11 . A device according to claim 9 or 10 , wherein the phase guide comprises a groove, ridge, or material with different wettability, that acts as a capillary pressure boundary, spanning the complete length of a moving fluid-fluid-meniscus, such that the meniscus is at least partially aligned along the phase guide.

12 . A device according to any one of claims 9 to 11 , wherein the channel comprises two phase guides that confine at a certain point in time during the filling process an advancing or receding liquid, wherein the phase guides differ in their stability for defining a sequential and/or selective overflow of the phase guides in predetermined order.

13 . A device according to any one of claims 9 to 12 , wherein the channel is essentially transparent.

14 . A device according to any one of claims 9 to 13 , wherein the distance between the channel walls (channel width) is in the range of from 100 μm to 2 mm.

15 . Use of the device according to any one of claims 10 to 14 for the enrichment of components, filtration of proteins and/or dust removal from biological samples.

16 . A process for the manufacturing of a device for the 2-phase electroextraction, comprising:
(a) providing a suitable inert base material, and (b) shaping the base material into a channel comprising at least two channel walls and at least one inlet and one outlet conduit, and a phase guide in the channel, and (c) providing the channel walls with electrodes.

Assignees

• Univ Leiden

Inventors

• Schoonen Jan-Williem
• Lindenburg Peter
• Van Duinen Vincent
• Oedit Amar
• Vulto Paul
• Hankemeier Thomas

Classifications

• G01N1/40

  Concentrating samples · CPC title

• B01L3/502776

  specially adapted for focusing or laminating flows · CPC title

• B01D11/0419 Primary

  in combination with an electric or magnetic field or with vibrations · CPC title

• B01D11/0496

  by extraction in microfluidic devices · CPC title

• B01L2300/0645

  Electrodes · CPC title