Methods for production of l-methionine and related products

Patent metadata

Field	Value
Publication number	US-2016108445-A1
Application number	US-201514982108-A
Country	US
Kind code	A1
Filing date	Dec 29, 2015
Priority date	Dec 29, 2010
Publication date	Apr 21, 2016
Grant date	—

Abstract

A method comprising: (a) enzymatically processing an O-acetylhomoserine (OAHS) fermentation liquor to produce L-methionine and an acetate source; (b) separating at least a portion of said L-methionine from at least a fraction of said acetate source to form separated L-methionine and a residual liquor comprising an acetate-source; and (c) recovering at least a portion of said acetate source from said residual liquor as recovered acetate.

First claim

1 - 71 . (canceled)

72 . A method for producing an L-methionine product comprising;
(a) providing a fermentation liquor comprising an L-methionine precursor selected from the group consisting of O-acetylhomoserine (OAHS) and O-succinyl homoserine (OSHS), and at least one impurity; (b) enzymatically processing said precursor to produce a reaction liquor comprising L-methionine, an organic acid selected from the group consisting of acetic acid and succinic acid and at least one impurity; (c) modifying said reaction liquor to form a crystallizing feed; (d) crystallizing L-methionine from said crystallizing feed to form crystalline L-methionine and an L-methionine-depleted mother liquor; and (e) recovering at least a portion of said organic acid from said mother liquor as recovered organic acid; wherein said recovering comprises producing a residual liquor containing said free organic acid and separating said free organic acid, and said crystalline L-methionine is characterized by at least one characteristic selected from the group consisting of: (i) purity greater than 95%; (ii) containing less than 1% organic acid; (iii) L-methionine precursor content greater than 1 PPB; (iv) content of at least one particular impurity is greater than 10 PPM; (v) less than 2% of D-methionine; and (vi) carbon-14 to carbon-12 ratio of at least 2.0×10 −13 .

73 . The method according to claim 72 , wherein said modifying comprises combining said reaction liquor with an L-methionine-comprising recycle stream.

74 . The method according to claim 73 , further comprising the step of separating L-methionine from said mother liquor to form said L-methionine-comprising recycle stream and a purged mother liquor stream.

75 . The method according to claim 74 , wherein said separating L-methionine from said mother liquor comprises crystallizing to form second crystalline L-methionine and separating said second crystalline L-methionine from said purged mother liquor.

76 . The method according to claim 72 , wherein one or more of said impurity is selected from the group consisting of amino acids, vitamins, minerals, ammonia, acetic acid, succinic acid, methyl mercaptan, enzymes, L-methionine precursor and a combination thereof.

77 . The method according to claim 72 , wherein said L-methionine precursor is O-succinyl homoserine, wherein said organic acid is succinic acid and wherein said processing is conducted in the presence of calcium ions.

78 . A composition comprising L-methionine, produced by the method of claim 72 and characterized by at least one characteristic selected from the group consisting of:
(i) containing less than 1% organic acid;
(ii) L-methionine precursor content greater than 1 PPB, wherein said precursor is selected from OAHS and OSHS;
(iii) content of at least one particular impurity is greater than 10 PPM; and
(iv) carbon-14 to carbon-12 ratio of at least 2.0×10 −13 .

79 . The composition according to claim 78 , characterized by L-methionine purity greater than 95%.

80 . A complex comprising: the composition of claim 78 or 79 ; and a heavy metal.

81 . A feed or food composition comprising the composition of claim 78 or 79 .

82 . A composition comprising L-methionine, produced by the method of claim 72 and characterized by at least one characteristic selected from the group consisting of:
(i) amino acids other than methionine in a concentration of at least 0.05%;
(ii) carboxylic acids in a concentration of at least 0.01%;
(iii) sulfate in a concentration of at least 0.2%;
(iv) OAHS in a concentration of at least 1 PPB; and
(v) methionine purity of at least 60%.

83 . A composition comprising L-methionine, produced by the method of claim 72 and characterized by at least one characteristic selected from the group consisting of:
(i) a methionine concentration of at least 15 g/L;
(ii) an acetate concentration of at least 100 g/L;
(iii) total solids of at least 20%; and
(iv) specific gravity in the range between 1.05 and 1.25; and
(v) ammonium sulfate concentration of at least 100 g/L.

84 . A composition comprising L-methionine, produced by the method of claim 72 and characterized by at least one characteristic selected from the group consisting of:
(i) a methionine concentration of at least 100 g/L;
(ii) an OAHS concentration less than 0.1 g/L;
(iii) a detectable amount of at least one amino acid selected from the group consisting of Glutamic acid, Valine, IsoLeucine, Leucine, Tyrosine, Phenylalanine, Threonine;
(iv) a mineral acid content of at least 30 g/L;
(v) an acetic acid content of at least 30 g/L;
(vi) a concentration of carboxylic acids other than acetic acid of at least 0.01%; and
(vii) a methanol concentration of at least 10% by weight.

85 . A method for producing an L-methionine comprising;
(a) enzymatically processing a fermentation liquor comprising an L-methionine precursor selected from the group consisting of O-acetylhomoserine (OAHS) and O-succinyl homoserine (OSHS) to produce L-methionine and a source of an organic acid selected from acetic acid and succinic acid; (b) separating at least a portion of said L-methionine from at least a fraction of said source of organic acid to form separated L-methionine and a residual liquor comprising the source of organic acid; and (c) recovering at least a portion of said source of organic acid from said residual liquor as recovered organic acid, wherein said recovering comprises producing said residual liquor containing free organic acid and separating said free organic acid.

86 . The method according to claim 85 , further comprising using said recovered organic acid as a reagent, wherein said using comprises applying to production of a fermentation product.

87 . The method according to claim 86 , wherein said fermentation product is selected from the group consisting of carboxylic and amino acids.

88 . The method according to claim 85 , further comprising using said recovered organic acid as a reagent, wherein said using comprises adding said reagent as an ingredient in a fermentation medium.

89 . The method according to claim 85 , wherein said L-methionine precursor is O-succinyl homoserine, wherein said organic acid is succinic acid, and wherein recovering comprises forming residual liquor comprising free succinic acid and separating said free succinic acid.

90 . A composition comprising the compound produced in step (c) of claim 85 , comprising:
at least 10% ammonium sulfate, and at least 10 PPM of ammonium acetate, isobutanol, isobutyl acetate, acetamide and methionine, respectively.

91 . A fertilizer comprising the composition of claim 90 .

Assignees

• Cj Cheiljedang Corp

Inventors

• Hong Soon Won
• Hwang In Seok
• Lee Sang Mok
• Lee Youn Jae
• Jung Jun Young
• Eyal Aharon

Classifications

• C12P13/12 Primary

  Methionine; Cysteine; Cystine · CPC title

• C07C319/28

  Separation; Purification · CPC title

• C05C3/00

  Fertilisers containing other salts of ammonia or ammonia itself, e.g. gas liquor · CPC title

• A23L33/175

  Amino acids · CPC title

• C05G3/00

  Mixtures of one or more fertilisers with additives not having a specially fertilising activity · CPC title