Novel metalloproteases

Patent metadata

Field	Value
Publication number	US-2016108388-A1
Application number	US-201414893837-A
Country	US
Kind code	A1
Filing date	May 29, 2014
Priority date	May 29, 2013
Publication date	Apr 21, 2016
Grant date	—

Abstract

Aspects of the present compositions and methods relate to novel metalloproteases polynucleotides encoding the novel metalloprotease, compositions and methods for use thereof.

First claim

1 . A polypeptide comprising an amino acid sequence having at least 60% sequence identity to the amino acid sequence selected from the group consisting of SEQ ID NOs: 3, 6, 9 and 14.

2 . The polypeptide of claim 1 , wherein said polypeptide has at least 80%, 95% or 98% sequence identity to the amino acid sequence selected from the group consisting of SEQ ID NOs: 3, 6, 9 and 14.

3 - 4 . (canceled)

5 . The polypeptide of claim 1 , wherein said amino acid sequence is the amino acid sequence of SEQ ID NO: 3, 6, 9 and/or 14.

6 - 11 . (canceled)

12 . The polypeptide of claim 1 , wherein said polypeptide has protease activity.

13 . The polypeptide of claim 12 , wherein said protease activity comprises casein hydrolysis, collagen hydrolysis, elastin hydrolysis, keratin hydrolysis, soy protein hydrolysis or corn meal protein hydrolysis.

14 . The polypeptide of claim 1 , wherein said polypeptide retains at least 50% of its maximal activity between pH 5 and 9.5 and/or between 30° C. and 80° C.

15 . (canceled)

16 . The polypeptide of claim 1 , wherein said polypeptide has cleaning activity in a detergent composition.

17 . The polypeptide of claim 16 , wherein said detergent composition is an ADW detergent composition, a laundry detergent composition, a liquid laundry detergent composition, or a powder laundry detergent composition.

18 - 21 . (canceled)

22 . The polypeptide of claim 1 , wherein said polypeptide is a recombinant polypeptide.

23 . A composition comprising the polypeptide of claim 1 .

24 . The composition of claim 23 , wherein said composition is a cleaning composition or a detergent composition.

25 . (canceled)

26 . The composition of claim 24 , wherein said detergent composition is selected from the group consisting of a laundry detergent, a fabric softening detergent, a dishwashing detergent, and a hard-surface cleaning detergent.

27 . The composition of claim 23 , wherein said composition further comprises a surfactant; at least one calcium ion and/or zinc ion; at least one stabilizer; from about 0.001 to about 0.1 weight % of said polypeptide; at least one bleaching agent; at least one adjunct ingredient; and/or one or more additional enzymes or enzyme derivatives selected from the group consisting of acyl transferases, alpha-amylases, beta-amylases, alpha-galactosidases, arabinosidases, aryl esterases, beta-galactosidases, carrageenases, catalases, cellobiohydrolases, celluloses, chondroitinases, cutinases, endo-beta-1, 4-glucanases, endo-beta-mannanases, esterases, exo-mannanases, galactanases, glucoamylases, hemicellulases, hyaluronidases, keratinases, laccases, lactases, ligninases, lipases, lipoxygenases, mannanases, oxidases, pectate lyases, pectin acetyl esterases, pectinases, pentosanases, peroxidases, phenoloxidases, phosphatases, phospholipases, phytases, polygalacturonases, proteases, pullulanases, reductases, rhamnogalacturonases, beta-glucanases, tannases, transglutaminases, xylan acetyl-esterases, xylanases, xyloglucanases, and xylosidases, additional metallopotease enzymes and combinations thereof.

28 - 37 . (canceled)

38 . The composition of claim 23 , wherein said composition is a granular, powder, solid, bar, liquid, tablet, gel, or paste composition.

39 - 41 . (canceled)

42 . A method of cleaning, comprising contacting a surface or an item with a cleaning composition comprising the polypeptide of claim 1 .

43 - 44 . (canceled)

45 . The method of claims 42 - 44 , wherein said item is dishware or fabric.

46 - 51 . (canceled)

52 . A method for producing the polypeptide of claim 1 comprising:
a. stably transforming a host cell with an expression vector comprising a polynucleotide encoding the polypeptide of claim 1 ;
b. cultivating said transformed host cell under conditions suitable for said host cell to produce said protease; and
c. recovering said protease.

53 - 59 . (canceled)

60 . A nucleic acid sequence comprising a nucleic acid sequence:
(i) having at least 70% identity to SEQ ID NO: 4, 10 or 15, or (ii) being capable of hybridizing to a probe derived from the polynucleotide sequence set forth in SEQ ID NO: 4, 10 or 15, under conditions of intermediate to high stringency, or (iii) being complementary to the polynucleotide sequence set forth in SEQ ID NO: 4, 10 or 15.

61 . A vector comprising the nucleic acid sequence of claim 60 .

62 . A host cell transformed with the vector of claim 61 .

63 - 64 . (canceled)

65 . A textile processing, animal feed, leather processing, feather processing, or corn soy protein processing composition comprising the polypeptide of claim 1 .

66 - 69 . (canceled)

Assignees

• Danisco Us Inc

Inventors

• Babe Lilia M
• Ghirnikar Roopa
• Goedegebuur Frits
• Gu Xiaogang
• Kolkman Marc
• Yao Jian
• Yu Shukun

Classifications

• C11D3/386

  Preparations containing enzymes {, e.g. protease or amylase} · CPC title

• C12N9/54 Primary

  bacteria being Bacillus · CPC title

• C12Y304/24

  Metalloendopeptidases (3.4.24) · CPC title

• C11D3/3945

  Organic per-compounds (C11D3/3902 takes precedence) · CPC title

• C12N9/52

  derived from bacteria {or Archaea} · CPC title