Culture observation apparatus and culture observation system

Patent metadata

Field	Value
Publication number	US-2016108353-A1
Application number	US-201514882954-A
Country	US
Kind code	A1
Filing date	Oct 14, 2015
Priority date	Oct 17, 2014
Publication date	Apr 21, 2016
Grant date	—

Abstract

An object is to lessen a troublesome checking task during cell culturing. A culture observation apparatus of the present invention includes: a light source unit that radiates illumination light onto the inside of a transparent culture vessel from a side surface of the culture vessel; an image acquisition unit that images, of the illumination light radiated from the light source unit, scattered light emitted from the inside of the culture vessel to acquire images; and a transmission unit that sends the images acquired by the image acquisition unit to the outside. The image acquisition unit includes a sensor array that has a plurality of sensors arranged in at least one line over approximately the entire length in one direction of a bottom surface of the culture vessel and a movement mechanism that relatively moves the sensor array and the culture vessel in a direction intersecting the array direction of the sensors and extending along the bottom surface.

First claim

1 . A culture observation apparatus comprising:
a light source unit that radiates illumination light onto an inside of a transparent culture vessel from a side surface of the culture vessel; an image acquisition unit that images, of the illumination light radiated from the light source unit, scattered light emitted from the inside of the culture vessel to acquire images; and a transmission unit that sends the images acquired by the image acquisition unit to the outside, wherein the image acquisition unit includes a sensor array that has a plurality of sensors arranged in at least one line over approximately the entire length in one direction of a bottom surface of the culture vessel and a movement mechanism that relatively moves the sensor array and the culture vessel in a direction intersecting the array direction of the sensors and extending along the bottom surface.

2 . A culture observation apparatus according to claim 1 , wherein the light source unit radiates illumination light along an optical axis that falls within a range of ±30° with respect to the horizontal direction.

3 . A culture observation apparatus according to claim 1 , wherein the light source unit makes illumination light enter at a height between the bottom surface of the culture vessel and a solution level of a culture solution reserved in the culture vessel.

4 . A culture observation apparatus according to claim 1 , wherein the sensors are each provided with a microlens that is disposed facing the bottom surface of the culture vessel and an image acquisition device that is disposed at the opposite side of the microlens from the bottom surface of the culture vessel.

5 . A culture observation apparatus according to claim 1 , wherein the movement mechanism moves the sensor array and the light source unit, and the culture vessel, relatively, in a direction intersecting the array direction of the sensors and extending along the bottom surface.

6 . A culture observation system comprising:
a culture observation apparatus according to claim 1 , which is disposed in an incubator; and a receiving unit that is disposed outside the incubator and that receives images sent from the transmission unit of the culture observation apparatus.

7 . A culture observation system according to claim 6 , further comprising a determination unit that determines culture states of cells based on the images received by the receiving unit.

Assignees

• Olympus Corp

Inventors

• Kimura Hiroyuki

Classifications

• C12M41/06

  of illumination · CPC title

• G01N21/47

  Scattering, i.e. diffuse reflection (G01N21/25, G01N21/41 take precedence {G01N21/55 takes precedence}) · CPC title

• C12M41/14

  Incubators; Climatic chambers (per se B01L1/00) · CPC title

• G01N2015/1479

  Using diffuse illumination or excitation · CPC title

• C12M31/10

  by light emitting elements located inside the reactor, e.g. LED or OLED · CPC title