Novel peptide selectively binding to volatile organic compounds

Patent metadata

Field	Value
Publication number	US-2016103108-A1
Application number	US-201514737648-A
Country	US
Kind code	A1
Filing date	Jun 12, 2015
Priority date	Oct 8, 2014
Publication date	Apr 14, 2016
Grant date	—

Abstract

Provided is a peptide selectively binding to a volatile organic compound, in which the peptide has excellent selectivity for the volatile organic compound and has stability at room temperature so as to effectively collect and detect or eliminate the volatile organic compound.

First claim

What is claimed is:

1 . A peptide or peptide set, comprising one or more selected from the group consisting of amino acid sequences of RX 2 X 2 SSX 3 P (SEQ ID NO. 1), X 1 SSPX 3 GA (SEQ ID NO. 2), SKX 1 SPX 2 T (SEQ ID NO. 3), X 1 X 2 SSX 2 MA (SEQ ID NO. 4), X 1 X 2 SGX 3 AA (SEQ ID NO. 5), X 2 TSSAX 3 P (SEQ ID NO. 6), RX 2 TX 2 SX 1 S (SEQ ID NO. 7), TX 1 X 3 TX 1 X 2 G (SEQ ID NO. 8), X 1 X 2 GTASS (SEQ ID NO. 9), AGX 1 X 2 SSA (SEQ ID NO. 10), X 1 SSPX 3 GA (SEQ ID NO. 11), SGTX 2 X 1 GP (SEQ ID NO. 12), SRX 2 X 3 X 2 MX 3 (SEQ ID NO. 13), X 2 PX 3 PTX 3 P (SEQ ID NO. 14), GX 2 MMAAP (SEQ ID NO. 15), and X 2 SAX 2 PX 3 P (SEQ ID NO. 16).

2 . The peptide or peptide set of claim 1 , wherein the peptide or peptide set selectively binds to volatile organic compounds.

3 . The peptide or peptide set of claim 2 , wherein the volatile organic compounds are hydrocarbons having a benzene ring.

4 . The peptide or peptide set of claim 1 , wherein the peptide or peptide set comprises one or more selected from the group consisting of amino acid sequences of SEQ ID NOS. 17 to 32.

5 . The peptide or peptide set of claim 1 , wherein the peptide or peptide set is displayed on a coat protein of a phage or a fragment thereof.

6 . The peptide or peptide set of claim 5 , wherein the phage is M13 phage, F1 phage, Fd phage, If1 phage, Ike phage, Zj/Z phage, Ff phage, Xf phage, Pf1 phage, or Pf3 phage.

7 . The peptide or peptide set of claim 5 , wherein the C-terminus of the peptide or peptide set is linked to the N-terminus of the coat protein of the phage, or the peptide or peptide set is inserted between consecutive amino acid sequences of the coat protein of the phage or replaces the consecutive amino acid sequences of the coat protein.

8 . The peptide or peptide set of claim 5 , wherein the coat protein is one or more selected from the group consisting of p3, p6, p8 and p9 of M13 phage.

9 . A device for detecting or eliminating a volatile organic compound, the volatile organic compound comprising the peptide or peptide set of claim 1 .

10 . The device of claim 9 , wherein the peptide or peptide set is displayed on a coat protein of a phage or a fragment thereof.

11 . The device of claim 9 , further comprising a substrate.

12 . A method of detecting or eliminating a volatile organic compound in a sample, the method comprising contacting the sample with the peptide or peptide set of claim 1 .

Assignees

• Korea Inst Sci & Tech

Inventors

• Yi Hyunjung
• Kim Sang Kyung
• Ju Soomi
• Lee Ki Young

Classifications

• C07K7/06

  having 5 to 11 amino acids · CPC title

• C12N7/00

  Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof (preparing medicinal viral antigen or antibody compositions, e.g. virus vaccines, A61K39/00) · CPC title

• G01N29/022

  Fluid sensors based on microsensors, e.g. quartz crystal-microbalance [QCM], surface acoustic wave [SAW] devices, tuning forks, cantilevers, flexural plate wave [FPW] devices (microdevices per se B81B) · CPC title

• G01N33/0004 Primary

  Gaseous mixtures, e.g. polluted air · CPC title

• C12N2795/14121

  Viruses as such, e.g. new isolates, mutants or their genomic sequences · CPC title