Tomato plants with improved agronomic traits

Patent metadata

Field	Value
Publication number	US-2016100538-A1
Application number	US-201514857688-A
Country	US
Kind code	A1
Filing date	Sep 17, 2015
Priority date	Sep 18, 2014
Publication date	Apr 14, 2016
Grant date	—

Abstract

The present disclosure provides tomato plants exhibiting improved fruit quality and disease resistance. Such plants may comprise novel introgressed genomic regions associated with improved fruit quality or disease resistance. In certain aspects, compositions, including novel polymorphic markers and methods for producing, breeding, identifying, and selecting plants or germplasm with an improved fruit quality or disease resistance phenotype are provided.

First claim

What is claimed is:

1 . A Solanum lycopersicum plant of a commercial variety, the plant comprising a Lin5 allele from Solanum pennellii and a Pen1 allele from Solanum pennellii , wherein the plant exhibits elevated Brix levels relative to a plant lacking the Lin5 and Pen1 alleles.

2 . The plant of claim 1 , wherein the Brix levels of the plant are equal to or greater than the levels of a plant comprising only the Lin5 allele added to the levels of a plant comprising only the Pen1 allele.

3 . The plant of claim 1 , wherein plant is homozygous for said Lin5 allele.

4 . The plant of claim 1 , wherein the plant is heterozygous for said Lin5 allele.

5 . The plant of claim 1 , wherein the plant is homozygous for said Pen1 allele.

6 . The plant of claim 1 , wherein the plant is heterozygous for said Pen1 allele.

7 . The plant of claim 1 , further comprising a Fr1 allele from Solanum peruvianum , and a Tm2a allele from Solanum peruvianum , wherein said plant exhibits improved resistance to Fusarium crown and root rot and tomato mosaic virus.

8 . The plant of claim 7 , wherein the plant is homozygous for said Fr1 allele from Solanum peruvianum.

9 . The plant of claim 7 , wherein the plant is homozygous for said Tm2a allele from Solanum peruvianum.

10 . The plant of claim 9 , wherein the plant exhibits decreased necrosis compared with a plant that is heterozygous for said Tm2a allele from Solanum peruvianum.

11 . The plant of claim 1 , wherein the plant is inbred.

12 . The plant of claim 1 , wherein the plant is hybrid.

13 . A plant part of the plant of claim 1 .

14 . The plant part of claim 13 , wherein the plant part is a leaf, root, flower, fruit, pollen, ovule, cell, or part thereof.

15 . A seed that produces the plant of claim 1 .

16 . The plant of claim 7 , comprising a recombined chromosomal segment comprising said Lin5 allele, said Fr1 allele, and said Tm2a allele, which confers improved resistance to Fusarium crown and root rot and tomato mosaic virus, and elevated Brix levels relative to a plant lacking said segment.

17 . A plant part of the plant of claim 16 .

18 . The plant part of claim 17 , wherein the plant part is a leaf, root, flower, fruit, pollen ovule, seed, cell, or part thereof.

19 . A seed that produces the plant of claim 16 .

20 . The plant of claim 16 , wherein a representative sample of seed comprising said chromosomal segment was deposited under ATCC Accession Number PTA-121480.

21 . The plant of claim 1 , comprising a Solanum lycopersicum allele at locus NL0217199, and a Solanum pennellii allele at locus NSLYC009229170, wherein the fruit of said plant exhibits elevated Brix levels relative to the fruit of a plant lacking the introgressed genomic region.

22 . A method for producing a hybrid Solanum lycopersicum plant with elevated Brix levels, comprising crossing a first Solanum lycopersicum parent plant with a second Solanum lycopersicum plant of a different genotype, wherein the first parent plant comprises a Lin5 allele from Solanum pennellii and a Pen1 allele from Solanum pennellii , wherein the Lin5 and Pen1 alleles confer elevated Brix levels relative to a plant lacking the Lin5 and Pen1 alleles.

23 . The method of claim 22 , further comprising producing a plurality of hybrid Solanum lycopersicum plants comprising crossing the first Solanum lycopersicum parent plant with a plurality of second Solanum lycopersicum plants of different genotypes.

24 . A method of identifying a tomato plant comprising a genotype associated with increased Brix levels in fruit, the method comprising the step of:
detecting in said plant at least a first polymorphism at locus NSLYC009194570.

Assignees

• Seminis Vegetable Seeds Inc

Inventors

• Aarden Harriette
• Bar Ami
• Van Den Bosch Bernardus
• Grit Albert
• Hotzev Amit
• Osri Arnon
• Rodriguez Maria Fernanda
• Rozier Bram
• Vecchietti Alberto
• Wagner Ruth

Classifications

• C12Q1/6895

  for plants, fungi or algae · CPC title

• C12Q2600/13

  Plant traits · CPC title

• C12Q2600/156

  Polymorphic or mutational markers · CPC title

• A01H5/08 Primary

  Fruits · CPC title

• A01H1/02

  Methods or apparatus for hybridisation; Artificial pollination {; Fertility} · CPC title