Identification and characterization of a peptide affinity reagent for the detection of noroviruses in clinical samples

Patent metadata

Field	Value
Publication number	US-2016061835-A1
Application number	US-201414777714-A
Country	US
Kind code	A1
Filing date	Mar 21, 2014
Priority date	Mar 21, 2013
Publication date	Mar 3, 2016
Grant date	—

Abstract

Embodiments of the disclosure include methods and/or compositions for the detection of viral infection, including at least Norovirus infection. In particular embodiments, there are methods and/or compositions employing particular peptides and/or phage useful for detecting Norovirus in a sample. The sample may be from an environment or from an individual. The individual may be a mammal, including a human, cow, horse, dog, cat, pig, and so forth. Certain exemplary peptides and phage that express the peptides are identified as useful for binding to Norovirus . Such peptides and phage are provided to one or more samples in order to identify whether or not Norovirus is present in the sample.

First claim

1 . A composition comprising one or both of:
a peptide comprising a sequence selected from the group consisting of the sequences in Table 1, or a functionally active variant thereof; and a phage that encodes the peptide or the variant.

2 . The composition of claim 1 , wherein the peptide is no more than about 50 amino acids in length.

3 . The composition of claim 1 , wherein the functionally active variant comprises one, two, or three or more alterations in SEQ ID NO:1.

4 . The composition of claim 3 , wherein the alterations are conservative amino acid substitutions.

5 . The composition of claim 1 , wherein the functionally active variant comprises sequence that is 80%, 85%, 90%, 95%, 97%, 98%, or 99% identical to the peptide of which it is a variant.

6 . The composition of claim 1 , wherein the peptide is labeled.

7 . The composition of claim 6 , wherein the label is fluorescent, radioactive, or colored.

8 . A composition comprising an antibody that recognizes a composition of claim 1 .

9 . A method of identifying Norovirus in a sample, comprising the step of subjecting the sample to the composition of claim 1 .

10 . The method of claim 9 , wherein the subjecting step comprises binding of the peptide to the Norovirus to produce a peptide/ Norovirus complex.

11 . The method of claim 10 , further comprising the step of subjecting the complex to a detectable antibody that recognizes the peptide.

12 . The method of claim 10 , wherein the peptide is labeled.

13 . The method of claim 9 , wherein the subjecting step comprises binding of the phage to the Norovirus to produce a phage/ Norovirus complex.

14 . The method of claim 13 , further comprising the step of subjecting the complex to a detectable antibody that recognizes the phage.

15 . The method of claim 13 , wherein the phage is labeled.

16 . The method of claim 9 , wherein the sample is an environmental sample.

17 . The method of claim 16 , wherein the environmental sample is from a medical facility, hotel, school, cruise ship, sports facility, grocery store, long-term care facility, camp, prison, dormitory, water source, a food, child day care, or adult day care.

18 . The method of claim 9 , wherein the sample is from an individual.

19 . The method of claim 18 , wherein the sample is a stool sample or vomitus sample.

20 . The method of claim 9 , further comprising the step of obtaining the sample.

21 . The method of claim 18 , further comprising the step of providing a therapy to the individual.

22 . A kit comprising the composition of claim 1 .

Assignees

• Baylor College Medicine

Inventors

• Palzkill Timothy Gerald
• Estes Mary K
• Atmar Robert Legare
• Rogers Jennifer Dawn
• Ajami Nadim Jose

Classifications

• G01N2333/08

  RNA viruses · CPC title

• A61K38/00

  Medicinal preparations containing peptides (peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00) · CPC title

• G01N2469/10

  Detection of antigens from microorganism in sample from host · CPC title

• G01N33/56983 Primary

  Viruses · CPC title

• C07K7/08

  having 12 to 20 amino acids (gastrins C07K14/595; somatostatins C07K14/655; melanotropins C07K14/68) · CPC title