Recombinant phages and proteins

Patent metadata

Field	Value
Publication number	US-2016018397-A1
Application number	US-201414332060-A
Country	US
Kind code	A1
Filing date	Jul 15, 2014
Priority date	Jul 15, 2013
Publication date	Jan 21, 2016
Grant date	—

Abstract

The present disclosure provides recombinant phages, a Wip1 p23 receptor binding protein and a Wip1 p24 receptor binding protein that bind to Bacillus anthracis . The disclosure further provides methods and uses thereof.

First claim

We claim:

1 . A recombinant protein composition comprising Wip1 p23 receptor binding protein, variants or fragments thereof.

2 . The recombinant protein composition according to claim 1 , wherein the recombinant protein composition further comprises Wip1 p24 receptor binding protein, variants or fragments thereof.

3 . The recombinant protein composition according to claim 1 , wherein the Wip1 p23 receptor binding protein further comprises a reporter molecule.

4 . The recombinant protein composition according to claim 4 , wherein the reporter molecule is a fluorophore, a fluorophore/quencher pair, an antibody, a llama-body, an isotope, or combinations thereof.

5 . The recombinant protein composition according to claim 1 , wherein the Wip1 p23 receptor binding protein is capable of binding Bacillus anthracis.

6 . The recombinant protein composition according to claim 1 , further comprising a substrate.

7 . The recombinant protein composition according to claim 1 , wherein the substrate is an array.

8 . A system for detecting Bacillus anthracis comprising a recombinant protein composition according to claim 1 , and a detector in communication with said recombinant protein composition, wherein the detector is capable of detecting a signal generated upon recognition of a Bacillus anthracis receptor by the recombinant protein composition.

9 . The system according to claim 8 , further comprising a light source in optical communication with the recombinant protein composition.

10 . The system according to claim 8 , further comprising a processor for processing signals detected by the detector.

11 . A method of identifying or detecting Bacillus anthracis in a sample, the method comprising: (a) providing a sample suspected of containing Bacillus anthracis ; (b) contacting the sample with a recombinant protein composition according to claim 1 , wherein a change in a signal generated by a reporter molecule indicates the presence of Bacillus anthracis in the sample.

12 . The method according to claim 11 , wherein the sample is a biological sample or environmental sample.

13 . The method according to claim 11 , wherein the reporter molecule is a fluorophore or flurophore/quencher pair.

14 . The method according to claim 11 , wherein the recombinant protein composition changes conformation when contacting a Bacillus anthracis receptor thereby changing detectable properties of the recombinant protein composition.

15 . An isolated nucleic acid encoding a Wip1 p23 receptor binding protein, a Wip1 p24 receptor binding protein, a variant thereof, or combination thereof, wherein the Wip1 p23 receptor binding protein, Wip1 p24 receptor binding protein, variants or combinations thereof bind to Bacillus anthracis.

16 . The isolated nucleic acid according to claim 15 , wherein the isolated nucleic acid is operably linked to a regulatory element, reporter, a detectable element, or combinations thereof.

17 . The isolated nucleic acid according to claim 15 , wherein the isolated nucleic acid is a cDNA.

18 . A recombinant expression vector comprising the isolated nucleic acid according to claim 15 .

19 . A recombinant expression composition comprising recombinant expression vector according to claim 18 .

20 . The recombinant expression composition according to claim 19 , wherein the recombinant expression composition further comprises a detectable element.

Assignees

• Univ Rockefeller

Inventors

• Fischetti Vincent
• Schuch Raymond
• Kan Sherry

Classifications

• C07K14/005 Primary

  from viruses · CPC title

• G01N33/56911 Primary

  Bacteria · CPC title

• G01N2333/705

  Assays involving receptors, cell surface antigens or cell surface determinants · CPC title

• C12N7/00

  Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof (preparing medicinal viral antigen or antibody compositions, e.g. virus vaccines, A61K39/00) · CPC title

• G01N21/6486

  Measuring fluorescence of biological material, e.g. DNA, RNA, cells (G01N21/6428 takes precedence) · CPC title