Trenched sample assembly for detection of analytes with electromagnetic read-write heads

Patent metadata

Field	Value
Publication number	US-2016018392-A1
Application number	US-201514869734-A
Country	US
Kind code	A1
Filing date	Sep 29, 2015
Priority date	Dec 16, 2010
Publication date	Jan 21, 2016
Grant date	—

Abstract

A sample assembly includes an outer layer with at least one sample trench. The sample trench includes a first set of antibodies that are bonded on a first surface of a base layer. Target antigens are bonded with the first set of antibodies, and a second set of antibodies are bonded to the target antigens. Further, the sample trench includes nanoparticles that are bonded to the second set of antibodies.

First claim

What is claimed is:

1 . A sample assembly including a biological sample having target antigens, the sample assembly comprising:
an outer layer having at least one sample trench, said sample trench having a bottom surface; a base layer; said sample trench including:
a first set of antibodies bonded on a first surface of said base layer;
said target antigens bonded with said first set of antibodies;
a second set of antibodies bonded to said target antigens; and
nanoparticles bonded to said second set of antibodies; wherein the first and second sets of antibodies are biologically identical.

2 . The sample assembly of claim 1 , comprising magnetic servo alignment marks.

3 . The sample assembly of claim 2 , wherein said sample assembly further comprises a servo alignment trench in said outer layer, said servo alignment trench parallel with said sample trench.

4 . The sample assembly of claim 3 , wherein said servo alignment trench is filled with tape ink and said magnetic servo alignment marks are formed in said tape ink.

5 . The sample assembly of claim 1 , wherein said base layer is formed on said bottom surface of said sample trench.

6 . The sample assembly of claim 1 , wherein said outer layer is formed on said base layer and said base layer is exposed by said bottom surface of said sample trench

7 . The sample assembly of claim 1 , wherein said outer layer is selected from the group consisting of diamond-like-carbon, polytetrafluoroethylene, aluminum oxide, and polyamides.

8 . The sample assembly of claim 1 , wherein said base layer is selected from the group consisting of gold, silicon, and silicon oxide.

Assignees

• IBM

Inventors

• Boday Dylan J
• Schwartz Stephen L
• Topol Anna W
• Waters Sandra L
• Winarski Daniel J

Classifications

• G01N33/54333 Primary

  Modification of conditions of immunological binding reaction, e.g. use of more than one type of particle, use of chemical agents to improve binding, choice of incubation time or application of magnetic field during binding reaction · CPC title

• G01N27/745

  for detecting magnetic beads used in biochemical assays (concerning the assays G01N33/54326; sensors therefor G01R33/1269; automatic analysers therefor G01N35/0098) · CPC title

• G01N33/54346

  Nanoparticles · CPC title

• G01N33/54366

  Apparatus specially adapted for solid-phase testing · CPC title

• B82Y15/00

  Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors · CPC title