Toxin genes and methods for their use

Patent metadata

Field	Value
Publication number	US-2016017363-A1
Application number	US-201414773015-A
Country	US
Kind code	A1
Filing date	Mar 6, 2014
Priority date	Mar 7, 2013
Publication date	Jan 21, 2016
Grant date	—

Abstract

Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a toxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated toxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:21-74, or the nucleotide sequence set forth in SEQ ID NO: 1-20, as well as variants and fragments thereof.

First claim

That which is claimed:

1 . A recombinant nucleic acid molecule comprising a nucleotide sequence encoding an amino acid sequence having pesticidal activity, wherein said nucleotide sequence is selected from the group consisting of:
a) the nucleotide sequence set forth in any of SEQ ID NO:1-20; b) a nucleotide sequence that encodes a polypeptide comprising the amino acid sequence of any of SEQ ID NO:21-74; c) a nucleotide sequence that encodes a polypeptide comprising an amino acid sequence having at least 95% sequence identity to the amino acid sequence of any of SEQ ID NO:21-74.

2 . The recombinant nucleic acid molecule of claim 1 , wherein said nucleotide sequence is a synthetic sequence that has been designed for expression in a plant.

3 . The recombinant nucleic acid molecule of claim 1 , wherein said nucleotide sequence is operably linked to a promoter capable of directing expression of said nucleotide sequence in a plant cell.

4 . An expression cassette comprising the recombinant nucleic acid molecule of claim 1 .

5 . The expression cassette of claim 4 , further comprising a nucleic acid molecule encoding a heterologous polypeptide.

6 . A host cell that contains the recombinant nucleic acid of claim 1 .

7 . The host cell of claim 6 that is a bacterial host cell.

8 . The host cell of claim 6 that is a plant cell.

9 . A transgenic plant comprising the host cell of claim 8 .

10 . The transgenic plant of claim 9 , wherein said plant is selected from the group consisting of maize, sorghum, wheat, cabbage, sunflower, tomato, crucifers, peppers, potato, cotton, rice, soybean, sugarbeet, sugarcane, tobacco, barley, and oilseed rape.

11 . A transgenic seed comprising the nucleic acid molecule of claim 1 .

12 . A recombinant polypeptide with pesticidal activity, selected from the group consisting of:
a) a polypeptide comprising the amino acid sequence of any of SEQ ID NO:21-74; and b) a polypeptide comprising an amino acid sequence having at least 95% sequence identity to the amino acid sequence of any of SEQ ID NO:21-74.

13 . The polypeptide of claim 12 further comprising heterologous amino acid sequences.

14 . A composition comprising the polypeptide of claim 12 .

15 . The composition of claim 14 , wherein said composition is selected from the group consisting of a powder, dust, pellet, granule, spray, emulsion, colloid, and solution.

16 . The composition of claim 14 , wherein said composition is prepared by desiccation, lyophilization, homogenization, extraction, filtration, centrifugation, sedimentation, or concentration of a culture of bacterial cells.

17 . The composition of claim 14 , comprising from about 1% to about 99% by weight of said polypeptide.

18 . A method for controlling a lepidopteran, hemipteran, coleopteran, nematode, or dipteran pest population comprising contacting said population with a pesticidally-effective amount of the polypeptide of claim 12 .

19 . A method for killing a lepidopteran, hemipteran, coleopteran, nematode, or dipteran pest, comprising contacting said pest with, or feeding to said pest, a pesticidally-effective amount of the polypeptide of claim 12 .

20 . A method for producing a polypeptide with pesticidal activity, comprising culturing the host cell of claim 6 under conditions in which the nucleic acid molecule encoding the polypeptide is expressed.

21 . A plant or plant cell having stably incorporated into its genome a DNA construct comprising a nucleotide sequence that encodes a protein having pesticidal activity, wherein said nucleotide sequence is selected from the group consisting of:
a) the nucleotide sequence set forth in any of SEQ ID NO:1-20; b) a nucleotide sequence that encodes a polypeptide comprising the amino acid sequence of any of SEQ ID NO:21-74; and c) a nucleotide sequence that encodes a polypeptide comprising an amino acid sequence having at least 95% sequence identity to the amino acid sequence of any of SEQ ID NO:21-74.

22 . A method for protecting a plant from a pest, comprising expressing in a plant or cell thereof a nucleotide sequence that encodes a pesticidal polypeptide, wherein said nucleotide sequence is selected from the group consisting of:
a) the nucleotide sequence set forth in any of SEQ ID NO:1-20; b) a nucleotide sequence that encodes a polypeptide comprising the amino acid sequence of any of SEQ ID NO:21-74; and c) a nucleotide sequence that encodes a polypeptide comprising an amino acid sequence having at least 95% sequence identity to the amino acid sequence of any of SEQ ID NO:21-74.

23 . The method of claim 22 , wherein said plant produces a pesticidal polypeptide having pesticidal activity against a lepidopteran, hemipteran, coleopteran, nematode, or dipteran pest.

24 . A method for increasing yield in a plant comprising growing in a field a plant of or a seed thereof having stably incorporated into its genome a DNA construct comprising a nucleotide sequence that encodes a protein having pesticidal activity, wherein said nucleotide sequence is selected from the group consisting of:
a) the nucleotide sequence set forth in any of SEQ ID NO:1-20; b) a nucleotide sequence that encodes a polypeptide comprising the amino acid sequence of any of SEQ ID NO:21-74; and c) a nucleotide sequence that encodes a polypeptide comprising an amino acid sequence having at least 95% sequence identity to the amino acid sequence of any of SEQ ID NO:21-74;
wherein said field is infested with a pest against which said polypeptide has pesticidal activity.

Assignees

• Athenix Corp
• Bayer Cropscience Lp

Inventors

• Thayer Rebecca
• Sampson Kimberly S
• Lehtinen Duane
• Peters Cheryl
• Roberts Kira
• Magalhaes Leonardo
• Dunn Ethan

Classifications

• A01N37/46

  N-acyl derivatives · CPC title

• C07K14/325 Primary

  Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins · CPC title

• C12N15/8286 Primary

  for insect resistance · CPC title

• A01N37/44

  containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids · CPC title

• Y02A40/146

  Genetically Modified [GMO] plants, e.g. transgenic plants · CPC title