Device and method for treating a filtration medium

Patent metadata

Field	Value
Publication number	US-2016017274-A1
Application number	US-201314418638-A
Country	US
Kind code	A1
Filing date	Jun 10, 2013
Priority date	Jul 31, 2012
Publication date	Jan 21, 2016
Grant date	—

Abstract

A device ( 1 ) and a method are provided for treating a porous filtration medium ( 37 ) having a receiving unit ( 2 ) with of a receiving part ( 5 ) and a base part ( 6 ). The porous filtration medium ( 37 ) can be lifted by the receiving part ( 5 ) from a lower part ( 33 ) of a filtration device ( 32 ), and the receiving part ( 5 ) with the porous filtration medium ( 37 ) can be mounted on the base part ( 6 ). The receiving part ( 5 ) is latchable to the base part ( 6 ). The base part ( 6 ), towards the filtration medium ( 37 ) has an incubation chamber ( 17 ) connected to a base part ( 6 ) outlet ( 3 ) that faces away from the receiving part ( 5 ), and the outlet ( 3 ) has a projection onto which a receiving vessel ( 4 ) containing a solvent ( 28 ) for dissolving the porous filtration medium ( 37 ) can be detachably pushed on.

First claim

1 . A device ( 1 ) for treating a porous filtration medium ( 37 ), the device ( 1 ) having a receiving unit ( 2 ) with a receiving part ( 5 ) and a base part ( 6 ), the porous filtration medium ( 37 ) is receivable via the receiving part ( 5 ) by a lower part ( 33 ) of a filtration device ( 32 ), and the receiving part ( 5 ) with the porous filtration medium ( 37 ) is mountable on the base part ( 6 ), and the receiving part ( 5 ) is formed so as to be latchable to the base part ( 6 ), wherein:
the base part ( 6 ), towards the filtration medium ( 37 ) has an incubation chamber ( 17 ) that is connected to an outlet ( 3 ) of the base part ( 6 ) that is facing away from the receiving part ( 5 ), and the outlet ( 3 ) has a projection ( 24 ) onto which a receiving vessel ( 4 ) containing a solvent ( 28 ) for dissolving the porous filtration medium ( 37 ) can be detachably pushed on.

2 . The device of claim 1 ,
wherein the incubation chamber ( 17 ) of the base part ( 6 ) is conical towards the outlet ( 3 ).

3 . The device of claim 1 ,
wherein the solvent ( 28 ) for dissolving the filtration medium ( 37 ) is an organic solvent.

4 . The device of claim 3 ,
wherein the solvent ( 28 ) is chloroform or methylene chloride.

5 . The device of claim 1 ,
wherein the receiving vessel ( 4 ) to be push-fitted to the base part ( 6 ) contains both the solvent ( 28 ) and the grinding balls ( 29 ) that support cell disruption.

6 . The device of claim 1 ,
wherein the receiving vessel ( 4 ) to be push-fitted to the base part ( 6 ) contains both the solvent ( 28 ) and a lysis buffer that supports cell disruption.

7 . The device of claim 1 ,
wherein an open end ( 26 ) of the receiving vessel ( 4 ) can be sealed with a cover to prevent fluids from leaking.

8 . The device of claim 5 wherein the outlet ( 3 ) of the base part ( 6 ) has an outlet channel ( 21 ) designed as an oblong slot arranged at a right angle to a longitudinal axis ( 22 ) of the base part ( 6 ), and having a clear width ( 23 ) smaller than an outside diameter of the grinding balls ( 29 ).

9 . The device of claim 1 ,
wherein an inner wall ( 8 ) of the receiving part ( 5 ) can be positioned outside a surface of the filtration medium ( 37 ) that can be used for filtration, and a fixing edge ( 11 ) arranged in the receiving part ( 5 ) can be positioned on an edge ( 38 ) of the filtration medium ( 37 ), and the fixing edge ( 11 ) of the receiving part ( 5 ) can be attached to the edge ( 38 ) of the filtration medium ( 37 ) by of an adhesive bond.

10 . The device of claim 1 ,
wherein the filtration medium ( 37 ) is made of polycarbonate or polyethersulfone.

11 . The device of claim 1 wherein the receiving unit ( 2 ) is attachable to the receiving vessel ( 4 ) arranged vertically at a bottom in a centrifuge adapter ( 39 ) and be centrifuged with the centrifuge adapter ( 39 )_in a centrifuge, whereby the filtration medium ( 37 ) dissolved in the solvent ( 28 ), including retained microorganisms, can be completely transferred into the receiving vessel ( 4 ).

12 . A method for treatment of a porous filtration medium ( 37 ) in a device ( 1 ) having a receiving unit ( 2 ) with a receiving part ( 5 ) and a base part ( 6 ), the method comprising:
mounting the receiving part ( 5 ) on the filtration medium ( 37 ), which is arranged in a lower part ( 33 ) of a filtration device ( 32 ) and is exposed to a liquid sample, wherein a fixing edge ( 11 ) arranged in the receiving part ( 5 ) is connected with an edge ( 38 ) of the filtration medium ( 37 ), lifting the receiving part ( 5 ) with the connected filtration medium ( 37 ) from the lower part ( 33 ), placing the receiving part ( 5 ) on the base part ( 6 ), and latching the receiving part ( 5 ) and the base part ( 6 ) together, detachably connecting a receiving vessel ( 4 ) containing a solvent ( 28 ) for dissolving the porous filtration medium ( 37 ) to an outlet ( 3 ) arranged on the base part ( 6 ), and that inverting and shaking the receiving unit ( 2 ) with the receiving vessel ( 4 ), whereby the solvent ( 28 ) is added to the filtration medium ( 37 ) via the outlet ( 3 ) of the base part ( 6 ), and dissolves the filtration medium ( 37 ).

13 . The method of claim 12 ,
further comprising: adding grinding balls ( 29 ) the receiving vessel ( 4 ) that contains the solvent ( 28 ).

14 . The method of claim 12 ,
further comprising: attaching the receiving unit ( 2 ) to the receiving vessel ( 4 ) arranged vertically at the bottom in a centrifuge adapter ( 39 ) and centrifuging in a centrifuge, whereby the filtration medium ( 37 ) dissolved in the solvent ( 28 ), including retained microorganisms, is completely transferred into the receiving vessel ( 4 ).

15 . The method of claim 14 ,
further comprising: removing the receiving vessel ( 4 ) from the receiving unit ( 2 ) and sealing by a cover.

16 . The method of claim 14 ,
further comprising: adding a lysis buffer to promote cell disruption being to the receiving vessel before sealing with the cover.

17 . The method of claim 16 ,
further comprising: processing the sealed receiving vessel ( 4 ) in an agitation incubator or homogenizer, with cell disruption of the microorganisms being facilitated by the lysis buffer.

Assignees

• Sartorius Stedim Biotech Gmbh

Inventors

• Pflanz Karl
• Scholz Alexandra

Classifications

• C12N1/02

  Separating microorganisms from their culture media · CPC title

• C12M47/06 Primary

  Hydrolysis; Cell lysis; Extraction of intracellular or cell wall material (lysis of microorganisms C12N1/06; extracting or separating nucleic acids from biological samples C12N15/1003) · CPC title

• C12M47/02

  Separating microorganisms from the culture medium; Concentration of biomass (separating microorganisms from their culture media C12N1/02) · CPC title

• C12Q1/6806

  Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay (C12Q1/6804 takes precedence) · CPC title