Microorganism having novel acrylic acid synthesis pathway having enhanced activity of coa acylating aldehyde dehydrogenase and method of producing acrylic acid using the same

Patent metadata

Field	Value
Publication number	US-2016010124-A1
Application number	US-201514620002-A
Country	US
Kind code	A1
Filing date	Feb 11, 2015
Priority date	Jul 8, 2014
Publication date	Jan 14, 2016
Grant date	—

Abstract

A microorganism capable of producing acrylic acid, comprising a genetic modification that increases activity of CoA acylating aldehyde dehydrogenase (ALDH) catalyzing conversion of 3-hydroxypropionaldehyde (3-HPA) to 3-hydroxy propionyl-CoA (3-HP-CoA) and a genetic modification that increases activity of 3-HP-CoA dehydratase catalyzing conversion of 3-HP-CoA to acrylyl-CoA in the microorganism in comparison with a cell that is not genetically engineered; as well as a method of producing the microorganism, and a method of producing acrylic acid using the same.

First claim

What is claimed is:

1 . A genetically engineered microorganism that produces acrylate, wherein the genetically engineered microorganism comprises
a genetic modification that increases CoA acylating aldehyde dehydrogenase (ALDH) activity in catalyzing conversion of 3-hydroxypropionaldehyde (3-HPA) to 3-hydroxy propionyl-CoA (3-HP-CoA); and a genetic modification that increases 3-HP-CoA dehydratase activity in catalyzing conversion of 3-HP-CoA to acrylyl-CoA; in comparison with a microorganism of the same type that is not genetically engineered.

2 . The microorganism of claim 1 , further comprises a genetic modification that increases activity of an enzyme that catalyzes conversion of acrylyl-CoA to acrylate in comparison with a microorganism of the same type that is not genetically engineered.

3 . The microorganism of claim 1 , wherein the ALDH has an amino acid sequence comprising one of SEQ ID NOs: 1 to 20.

4 . The microorganism of claim 1 , wherein the ALDH belongs to EC 1.2.1.10, or EC 1.2.1.87.

5 . The microorganism of claim 1 , wherein the ALDH is propionaldehyde dehydrogenase (pduP).

6 . The microorganism of claim 1 , wherein the 3-HP-CoA dehydratase has an amino acid sequence comprising one of SEQ ID NOs: 41 to 119.

7 . The microorganism of claim 1 , wherein the 3-HP-CoA dehydratase belongs to EC 4.2.1.

8 . The microorganism of claim 2 , wherein the enzyme that catalyzes conversion of acrylyl-CoA to acrylate has an amino acid sequence comprising one of SEQ ID NOs: 199 to 204.

9 . The microorganism of claim 2 , wherein the enzyme that catalyzes conversion of acrylyl-CoA to acrylate belongs to EC 3.2.1.

10 . The microorganism of claim 2 , wherein the enzyme that catalyzes conversion of acrylyl-CoA to acrylate is 3-HP-CoA hydrolase or 3-hydroxyisobutyryl-CoA hydrolase.

11 . The microorganism of claim 1 , wherein the genetically engineered microorganism comprises increased activity of ALDH and 3-HP-CoA dehydratase and the increased activity of ALDH and 3-HP-CoA dehydratase is caused by increased expression of polynucleotides encoding the enzymes as compared to a microorganism of the same type that is not genetically engineered.

12 . The microorganism of claim 1 , wherein the genetically engineered microorganism comprises exogenous polynucleotides encoding ALDH, 3-HP-CoA dehydratase, and an enzyme catalyzing conversion of acrylyl-CoA to acrylate.

13 . The microorganism of claim 1 , wherein the microorganism is of the Enterobacteria, Corynebacterium , or Brevibacterium genera.

14 . The microorganism of claim 1 , wherein a gene encoding at least one enzyme involved in a pathway of degrading acrylate or converting acrylate to another product is deleted or disrupted.

15 . The microorganism of claim 1 , wherein the genetically engineered microorganism produces 3-HPA.

16 . The microorganism of claim 15 , wherein the genetically engineered microorganism is E. coli that produces 3-HPA, and comprises an exogenous gene encoding glycerol dehydratase (GDH) and an exogenous gene encoding glycerol dehydratase reactivase (GDR).

17 . A method of producing acrylate, the method comprising culturing the microorganism of claim 1 in a culture medium.

18 . The method of claim 17 , wherein the method further comprises recovering acrylate from the culture.

19 . A method of producing a genetically engineered microorganism according to claim 1 , the method comprising introducing into a microorganism an exogenous polynucleotide encoding CoA acylating aldehyde dehydrogenase (ALDH), and an exogenous polynucleotide encoding 3-HP-CoA dehydratase.

Assignees

• Samsung Electronics Co Ltd

Inventors

• Nam Taewook
• Song Yeoju
• Song Seunghoon
• Yun Jiae
• Lee Jinsuk
• Chu Hunsu
• Choi Insuk

Classifications

• C12N9/88

  Lyases (4.) · CPC title

• C12N9/2402

  hydrolysing O- and S- glycosyl compounds (3.2.1) · CPC title

• C12N15/70

  Vectors or expression systems specially adapted for E. coli · CPC title

• C12Y102/0101

  Acetaldehyde dehydrogenase (acetylating) (1.2.1.10) · CPC title

• C12N9/0008

  acting on the aldehyde or oxo group of donors (1.2) · CPC title