High linolenic acid producing brassica plants

Patent metadata

Field	Value
Publication number	US-2016010096-A1
Application number	US-201514694747-A
Country	US
Kind code	A1
Filing date	Apr 23, 2015
Priority date	Oct 28, 2013
Publication date	Jan 14, 2016
Grant date	—

Abstract

The present disclosure is directed to Brassica plants having elevated levels of linolenic acids, including non-transgenic plants having elevated levels of linolenic acids. Also described herein are methods for the production of those plants and oils derived from such plants.

First claim

1 . Seed of Brassica napus, Brassica oleracea , or Brassica juncea , comprising all or part of the genomic sequence of B. napus line rrm1367-003 between SNP markers: C2-p16531874 and C2-p51360247;
wherein said part of the genomic sequence is greater than 200 base pairs.

2 . The seed of claim 1 comprising all or part of the genomic sequence between any two SNP markers selected from the group consisting of:
C2-p25019477, C2-p25478505, C2-p25656807, C2-p25913678, C2-p26147167, C2-p26159348, C2-p26207733, C2-p27157822, C2-p27601989, C2-p28031338, C2-p28070964, C2-p28698152, C2-p28806917, C2-p29076828, C2-p29348165, C2-p29383684, 5C00434-p169753, C2-p29474845, C2-p29474845, C2-p29505033, C2-p29505741, C2-p29607300, C2-p29984659, C2-p30062266, C2-p30070472, C2-p30110169, C2-p30154901, C2-p30162991, C2-p30402845, C2-p30431524, C2-p30771286, C2-p30902832, C2-p30942623, C2-p31035160, C2-p31230778, C2-p31354336, C2-p31475220, C2-p31485080, C2-p31502391, C2-p31807771, C2-p31985379, C2-p32008623, C2-p32147720, C2-p32588191, C2-p3353696791, C2-p33633673, C2-p33653822, C2-p33745239, C2-p33761702, C2-p33897506, C2-p33982349, C2-p34550916, C13529254-p142, C2-p34723961, C2-p34766378, C2-p35082231, C2-p35629571, C2-p36261423, C2-p36532052, C2-p36905514, C2-p37181623, C2-p38415038, A02-p21713756 A02-p25181726, and C2-p51360247.

3 . Seed of Brassica napus, Brassica oleracea , or Brassica juncea , comprising all or part of the genomic sequence of B. napus line rrm1367-003 between SNP markers: C7-p4690293 and C7-p22870500.

4 . The seed of claim 3 comprising all or part of the genomic sequence between any two SNP markers selected from the group consisting of: C7-p4690293, C7-p5039845, C7-p5194981, C7-p7498659, C7-p8599974, C7-p8719053, C7-p8726636, C7-p8726743, C7-p8727745, C7-p8766230, C7-p8824122, C7-p8854349, C7-p8870860, C7-p9307503, C7-p9358459, C7-p9593996, C7-p10040604, C7-p10165832, C7-p10180076, C7-p10180716, C7-p10212158, C7-p10215060, C7-p10215325, C7-p10228536, C7-p10261396, C7-p10262047, C7-p10613314, C7-p10617039, C7-p10720977, C7-p11706153, C7-p11718201, C7-p12072579, C7-p12079142, C7-p12123100, C7-p12123399, C7-p12268682, C7-p12281546, C7-p12300699, C7-p12300699, C7-p12301957, C7-p12356302, C7-p12356455, C7-p12385657, C7-p12387173, C7-p12401233, C7-p12485308, C7-p12508706, C7-p12512146, C7-p12514520, C7-p12565005, C7-p12684624, C7-p12757060, C7-p12984513, C7-p12990275, C7-p12995305, C7-p13029440, C7-p13029555, C7-p13069990, C7-p13070860, C7-p13083371, C7-p13135120, C7-p22861548, C7-p22870500, and C7-p22897297.

5 . The seed of claim 3 , comprising all or part of the genomic sequence of B. napus rrm1367-003 between SNP markers: C2-p1653187 and C2-p51360247; and/or C7-p4690293 and C7-p22897297, which genomic sequence when introduced B. napus cv. Topas, ATCC deposit PTA-120738, results in an increase in the 18:3 content of the seed oil fraction of seeds produced by the plant into which the fragment has been introduced (e.g., by breeding) relative to B. napus cv. Topas grown under the same or substantially the same conditions.

6 . The seed of claim 3 , wherein at least one part of the genomic sequence of B. napus rrm1367-003 present in said seed has a length greater than 500 base pairs.

7 . The seed of claim 1 , wherein the alpha linolenic acid content is greater than 1.4 times higher than a reference strain selected from: B. napus cv. Topas; or B. napus cv. AV-Sapphire, breeders code RO011; wherein said reference strain is grown under the same or substantially the same conditions, and said seed is harvested under the same or substantially the same conditions.

8 . The seed of claim 1 , wherein the alpha linolenic acid content is greater than 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2.0, 2.1, 2.2, or 2.3 times higher than the reference strain B. napus cv. Topas, wherein said reference strain is grown under the same or substantially the same conditions, and said seed is harvested under the same or substantially the same conditions.

9 . The seed of claim 1 , wherein the alpha linolenic acid content is greater than 1.4, 1.5, or 1.6 times higher than the reference strain B. napus cv. AV-Sapphire, breeders code RO011, wherein said reference strain is grown under the same or substantially the same conditions, and said seed is harvested under the same or substantially the same conditions.

10 . The seed of claim 1 , wherein the seed has an oil fraction with an 18:3 fatty acid content greater than 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, or 22 percent by weight of the oil fraction, or in a range selected from 7-9, 9-12, 12-15, 15-19, or 19-23 percent by weight of the oil fraction.

11 . The seed of claim 1 , wherein the seed has an oil fraction with a linolenic acid content in a range selected from 26-24, 24-22, 22-20, 20-16, 19-15, 18-15, or 17-14 percent by weight.

12 . The seed of claim 1 , wherein the seed has an oil fraction with an oleic acid content less than 69, 68, 66, 64, 62, 61,60, 58, 56, 54, 52, 50, 48, 46, 44, or 42 percent by weight or in a range selected from 69-60, 65-53, 60-50, or 50-41 percent by weight.

13 . The seed of claim 1 , wherein the seed has an oil fraction with:
a linolenic acid content is greater than 16 percent by weight; an 18:1 fatty acid content in a range selected from 41-50, 45-55, or 50-60 percent by weight; and an 18:2 fatty acid in a range selected from 15-20 or 20-24 percent by weight.

14 . The seed of claim 1 , wherein the seed has an oil fraction with:
a linolenic acid content is greater than 17 percent by weight; an 18-1 fatty acid content in a range selected from 44-50, 46-55, 45-56, 50-55, or 50-57 percent by weight; and an 18:2 fatty acid in a range selected from 15-20 or 20-24 percent by weight.

15 . The seed of claim 1 , having less than 2, 1, 0.5, or 0.1 percent erucic acid by weight of the seed oil fraction.

16 . A plant grown from the seed claim 1 or a part thereof, wherein said plant is non-transgenic, transgenic, or transgenic subject to the proviso that the only transgenes present are genes for herbicide resistance.

17 . A plant, part thereof according to claim 16 expressing a herbicide tolerance to a herbicide selected from the group consisting of imidazolinone, dicamba, cyclohexanedione, sulfonylurea, glyphosate, glufosinate, phenoxy propionic acid, L-phosphinothricin, triazine and benzonitrile.

18 . A plant, part thereof, cell, or protoplast of claim 16 , having insect resistance conferred by a gene encoding a Bacillus thuringiensis endotoxin which is expressed in said plant, part thereof, cell, or protoplast.

19 . The plant of claim 16 , wherein the seed has a meal fraction that contains less than 10, 15, 20, 25, 30, 35, or 40 micromoles of any one or more of 3-butenyl glucosinolate, 4-pentenyl glucosinolate, 2-hydroxy-3 butenyl glucosinolate, and 2-hydroxy-4-pentenyl glucosinolate per gram of dry (air-dry), oil-free solid.

20 . Oil from a seed of claim 1 , or from a plant, or part thereof, grown from a seed of claim 1 , wherein said oil comprises nucleic acids having all or part of the genomic sequence of B. napus line rrm1367-003.

21 . Brassica napus= 1367-003 deposited as ATCC Accession number PTA-120636, or a progeny thereof having an oil fraction with a linolenic acid content greater than about 16, 17, 19, 20, or 21 percent by weight.

22 . A non-transgenic B. napus, B. olereca , or B. juncea plant, or parts thereof, having a low-saturated-fat trait that produces seed having an

Assignees

• Cargill Inc

Inventors

• Chen Zhizheng
• Zheng Honggang

Classifications

• A01H5/10 Primary

  Seeds · CPC title

• C12N15/8274

  for herbicide resistance · CPC title

• C12N15/8278

  Sulfonylurea · CPC title

• C12N15/8275

  Glyphosate · CPC title

• C12N15/8286

  for insect resistance · CPC title