Devices and methods for forming relatively monodisperse droplets

Patent metadata

Field	Value
Publication number	US-2016008778-A1
Application number	US-201414767798-A
Country	US
Kind code	A1
Filing date	Mar 5, 2014
Priority date	Mar 6, 2013
Publication date	Jan 14, 2016
Grant date	—

Abstract

Devices and methods for dividing droplets are generally described. In some embodiments, an article may comprise a fluidic channel comprising an array of obstructions. In certain embodiments, the arrangement of obstructions in the array may affect the flow path of fluid in the channel. For example, the array of obstructions may be used to convert a polydisperse population of droplets into a relatively monodisperse population of droplets. Passing a polydisperse population of droplets through the array may result in the division of droplets such that the population of droplets exiting the array has a narrower distribution in the characteristic dimensions of the droplets. The arrangement of obstructions in the array may allow for high-throughput production of a substantially monodisperse population of droplets in some cases. In some embodiments, the population of droplets exiting the array may be converted into particles.

First claim

What is claimed is:

1 . An article, comprising:
a microfluidic channel comprising a two-dimensional array of obstructions therein, arranged in a plurality of rows of substantially regularly-spaced obstructions, the rows arranged substantially orthogonal to a direction of average fluid flow through the microfluidic channel, wherein at least some of the rows of substantially regularly-spaced obstructions are offset relative to an adjacent row of substantially regularly-spaced obstructions.

2 . The article of claim 1 , wherein the average horizontal spacing between an obstruction and a next nearest obstruction in the rows of the array is greater than or equal to about 10 micrometers and less than about 100 micrometers.

3 . The article of any one of claims 1 or 2 , wherein the average vertical spacing between an obstruction and a next nearest obstruction in the columns of the array is greater than or equal to about 10 micrometers and less than about 100 micrometers.

4 . The article of any one of claims 1 - 3 , wherein the centers of the obstructions in at least some of the rows are offset relative to the centers of the obstructions in an adjacent row.

5 . The article of claim 4 , wherein the centers of the obstructions in at least some of the rows are offset relative to the centers of the obstructions in an adjacent row by less than or equal to about 100 micrometers.

6 . The article of any one of claims 1 - 5 , wherein the array of obstructions comprises at least 5 rows and less than 100 rows of obstructions.

7 . The article of any one of claims 1 - 6 , wherein at least some of the obstructions have a portion that is at a 90° angle with respect to an average direction of fluid flow in the microfluidic channel.

8 . The article of any one of claims 1 - 7 , wherein at least some of the obstructions are substantially rectangular.

9 . The article of any one of claims 1 - 8 , wherein at least some of the obstructions are substantially square.

10 . The article of any one of claims 1 - 9 , wherein at least some of the obstruction are substantially circular.

11 . The article of any one of claims 1 - 10 , wherein the average height of the obstructions is less than about 100 micrometers.

12 . The article of any one of claims 1 - 11 , wherein the average width of the obstructions is less than about 100 micrometers.

13 . The article of any one of claims 1 - 12 , wherein the average aspect ratio of the obstructions is at least 2.

14 . The article of any one of claims 1 - 13 , wherein the average aspect ratio of the obstructions is less than about 10.

15 . The article of any one of claims 1 - 14 , wherein the average interstitial volume of the array is less than or equal to about 200,000 cubic micrometers.

16 . An article, comprising:
a microfluidic channel comprising a two-dimensional array of obstructions therein, arranged in a plurality of rows of obstructions, the rows arranged substantially orthogonal to a direction of average fluid flow through the microfluidic channel, wherein at least about 90% of imaginary lines drawn through the array of obstructions in the direction of average fluid flow through the microfluidic channel intersects obstructions of at least about 40% of the rows of obstructions forming the array.

17 . An article, comprising:
a microfluidic channel comprising an array of obstructions therein, arranged such that no flow path of fluid from upstream entering the array of obstructions exits downstream of the array without at least five changes in direction.

18 . A method, comprising:
providing a two-dimensional array of obstructions contained within a microfluidic channel, wherein the average distance between an obstruction and the next nearest obstruction is less than about 1 mm; and passing a plurality of droplets through the array of obstructions to divide at least about 50% of the droplets to form a plurality of divided droplets.

19 . The method of claim 18 , wherein substantially all of the droplets are divided to form the plurality of divided droplets.

20 . The method of any one of claims 18 or 19 , wherein the plurality of divided droplets has a coefficient of variation of the characteristic dimension is less than or equal to about 20%.

21 . The method of any one of claims 18 - 20 , wherein the coefficient of variation of the characteristic dimension of each of the plurality of droplets is greater than the coefficient of variation of the characteristic dimension of each of the plurality of divided droplets.

22 . The method of any one of claims 18 - 21 , wherein at least about 70% of the droplets are divided to form the plurality of divide droplets.

23 . The method of any one of claims 18 - 22 , wherein at least about 90% of the droplets are divided to form the plurality of divide droplets.

24 . The method of any one of claims 18 - 23 , wherein the droplets are contained within a liquid.

25 . The method of any one of claims 18 - 24 , wherein the ratio of the viscosity of the droplets to the viscosity of the liquid is less than or equal to about 20.

26 . The method of any one of claims 18 - 25 , wherein the capillary number of the droplets is less than about 2.

27 . A method, comprising:
applying shear forces to a plurality of droplets by passing the plurality of droplets through a two-dimensional array of obstructions such that the droplets are divided to form a plurality of divided droplets, wherein the plurality of divided droplets has a distribution in characteristic dimension such that no more than about 5% of the divided droplets have a characteristic dimension greater than about 120% or less than about 80% of the average characteristic dimension of the plurality of divided droplets.

28 . The method of claim 27 , wherein the shear stress is greater than or equal to about 0.01 Pa and less than about 3 Pa.

29 . A method, comprising:
passing a droplet through a two-dimensional array of obstructions contained within a microfluidic channel to divide the droplet to form a plurality of divided droplets.

Assignees

• Harvard College

Inventors

• Weitz David A
• Amstad Esther
• Sperling Ralph Alexander

Classifications

• B01L3/502746 Primary

  characterised by the means for controlling flow resistance, e.g. flow controllers, baffles or throttle valves · CPC title

• B01F5/0602

  Operations & Transport · mapped topic

• B01F3/0807

  Operations & Transport · mapped topic

• B01F13/0069 Primary

  Operations & Transport · mapped topic

• B01F5/0661

  Operations & Transport · mapped topic