Phytase formulation

Patent metadata

Field	Value
Publication number	US-2016007633-A1
Application number	US-201414770766-A
Country	US
Kind code	A1
Filing date	Mar 10, 2014
Priority date	Mar 14, 2013
Publication date	Jan 14, 2016
Grant date	—

Abstract

A liquid enzyme formulation, an enzyme granule formulation, methods for manufacturing enzyme granules using a fluid bed dryer, wherein the enzyme granules are thermostable without the need for a thermostable coating is provided. The enzyme granules are phytase granules used in the manufacturing of an animal feed, wherein the phytase granule is thermostable without the need for a thermostable coating and the phytase retains about 63% to about 134% of its activity after pelleting at 80° C.

First claim

What is claimed is:

1 . A liquid enzyme formulation comprising:
(a) a phytase; (b) a buffer, wherein the buffer is selected from the group consisting of: a sodium citrate, potassium citrate, citric acid, sodium acetate, acetic acid, sodium phosphate, potassium phosphate and any combination thereof; (c) a stabilizer, wherein the stabilizer is selected from the group consisting of: a sucrose, a sorbitol, a mannitol, a glycerol, a trehalose, a sodium chloride, a sodium sulphate, or any combination thereof; and, (d) an anti-microbial, wherein the anti-microbial is selected from the group consisting of: a potassium sorbate, sodium sorbate, a sorbic acid, a sodium benzoate, a benzoic acid, a methyl paraben, a calcium propionate, a sodium propionate, an ammonium propionate, a propionic acid, or any combination thereof.

2 . The liquid enzyme formulation of claim 1 , wherein
(a) the buffer has a pH in a range from about 4.5 to about 5.50, (b) the stabilizer is a combination of at least two stabilizers, (c) the stabilizer is at a concentration between about 5% to about 30%, or (d) the anti-microbial is a combination of at least two anti-microbials.

3 .- 22 . (canceled)

23 . A granulated enzyme formulation comprising:
(a) a carrier; (b) a primary solution comprising a phytase, a buffer, a stabilizer, a binding agent, a first plasticizer, an anti-microbial, or any combination thereof; and (c) a secondary solution comprising an enhancer, a second plasticizer, or any combination thereof.

24 . The granulated enzyme formulation of claim 23 , wherein the carrier is selected from the group consisting of:
(a) a flour; (b) a wheat flour; (c) a bleached flour (d) a wheat flour and a maltodextrin; (e) a wheat flour and a pre-gelatinized starch; and (f) a starch.

25 . The granulated enzyme formulation of claim 23 , wherein
(a) the buffer is a sodium citrate, (b) the stabilizer is selected from the group consisting of: a sucrose, a sorbitol, a mannitol, a glycerol, a trehalose, a sodium chloride, a sodium sulphate, kaolin, aluminum silicate, magnesium silicate, and any combination thereof, (c) the binding agent is selected from the group consisting of: a guar gum, a xanthan, a sodium algenate, a locust bean gum, a carrageenan gum, a pre-gelatinized modified starch, a maltodextrin, gelatin, a methyl cellulose, a hydroxypropyl cellulose, a hydroxypropyl methyl cellulose, a carboxymethyl cellulose, and any combination thereof, (d) the binding agent is selected from the group consisting of: a guar gum, a xanthan, a sodium algenate, a locust bean gum, a carrageenan gum, a pre-gelatinized modified starch, a maltodextrin, gelatin, a methyl cellulose, a hydroxypropyl cellulose, a hydroxypropyl methyl cellulose, a carboxymethyl cellulose, and any combination thereof, (e) the first plasticizer is selected from the group consisting of: a glycerol, polyethylene glycol, triethyl citrate, triacetin, and acetyl triethylcitrate, (f) the anti-microbial is selected from the group consisting of: a potassium sorbate, sodium sorbate, sodium benzoate, sodium propionate, calcium propionate, ammonium propionate, methyl paraben, benzoic acid, sorbic acid, and any combination thereof. (g) the enhancer is selected from the group consisting of: pre-gelatinized modified starch, maltodextrin, sodium algenate, carrageenan with CaCl2, carregeenan without CaCl2, guar gum with sodium borate, guar gum without sodium borate, gelatin; methyl cellulase; hydroxypropyl cellulase; hydroxypropyl methyl cellulase; carboxymethyl cellulase; sodium chloride; sodium sulphate; kaolin; aluminum silicate, magnesium silicate, and any combination thereof, or (h) the second plasticizer is selected from the group consisting of a glycerol, polyethylene glycol, triethyl citrate, triacetin, and acetyl triethylcitrate.

26 . The granulated enzyme formulation of claim 23 , 24 or 25 , further comprising a flowing aid, wherein the flowing aid is selected from the group consisting of: silicon dioxide, magnesium stearate, kaolin, talc, diatomaceous earth, and any combination thereof.

27 . A method for manufacturing an enzyme granule in a fluidized bed, wherein the enzyme retains at least 63% activity at 75° C. comprising:
(a) loading a dry carrier in a fluid bed granulator;
(b) adding a primary enzyme solution to the dry carrier; and
(c) drying the combination of (a) and (b) to a desired moisture content.

28 . The method of claim 27 , further comprising adding a secondary granulation solution to (a) and (b).

29 . The method of claim 27 , further comprising (d) adding a flowing aid to the fluid bed dryer or dry blender after drying.

30 . The method of claim 28 , wherein the secondary granulation solution further comprises a flowing aid.

31 . The method of claim 27 , wherein the enzyme is selected from the group consisting of: a Phytase, a cellulase, lactase, lipase, protease, catalase, xylanase, beta-glucanase, mannanase, amylase, amidase, epoxide hydrolase, esterase, phospholipase, transaminase, amine oxidase, cellobiohydrolase, ammonia lyase, and any combination thereof.

32 . The method of claim 27 , wherein the desired moisture content is between about 3% to about 10%.

33 . A formulation for phytase wherein the phytase retains an activity at a temperature comprising:
(a) at least 63% activity at 80° C.; (b) at least 58% activity at 85° C.; (c) at least 43% activity at 88° C.; (d) at least 27% activity at 90° C.; and (e) at least 6% activity at 93° C.

Assignees

• Basf Enzymes Llc

Inventors

• Han Yun
• Pratt Michael
• Wu Yi

Classifications

• C12Y301/03072

  5-Phytase (3.1.3.72) · CPC title

• C12Y301/03008

  3-Phytase (3.1.3.8) · CPC title

• C12N9/16 Primary

  acting on ester bonds (3.1) · CPC title

• B01J2/16 Primary

  by suspending the powder material in a gas, e.g. in fluidised beds or as a falling curtain · CPC title

• C12Y301/03026

  4-Phytase (3.1.3.26), i.e. 6-phytase · CPC title