High frame-rate multichannel beam-scanning microscopy

Patent metadata

Field	Value
Publication number	US-2016004060-A1
Application number	US-201514735959-A
Country	US
Kind code	A1
Filing date	Jun 10, 2015
Priority date	Jun 10, 2014
Publication date	Jan 7, 2016
Grant date	—

Abstract

A beam-scanning optical design is described for achieving up to kHz frame-rate optical imaging on multiple simultaneous data acquisition channels. In one embodiment, two fast-scan resonant mirrors direct the optical beam on a circuitous trajectory through the field of view, with the trajectory repeat-time given by the least common multiplier of the mirror periods. Dicing the raw time-domain data into sub-trajectories combined with model-based image reconstruction (MBIR) 3D in-painting algorithms allows for effective frame-rates much higher than the repeat time of the Lissajous trajectory. Because sub-trajectory and full-trajectory imaging are different methods of analyzing the same data, both high-frame rate images with relatively low resolution and low frame rate images with high resolution are simultaneously acquired.

First claim

1 . A system for beam-scanning and imaging, comprising:
two synchronized fast-scanning resonant mirrors of differing resonant frequencies to scan the beam in a Lissajous trajectory across a sample surface; implementation of a data acquisition and analysis technique for binning the image data into user-defined temporal bins, resulting in user-defined frame-rates; and a strategy for interpolating the values of unsampled pixels using image interpolation ‘inpainting’ techniques, which include but is not limited to model-based image reconstruction (MBIR) and discrete cosine transform (DCT) coupled with a penalized least squares (DCT-LS), the image interpolation may use some or all of the available dimensions of information, including spatial information, temporal information, polarization information, and spectral information.

2 . The system of claim 1 , the system is capable of achieving up to kHz frame-rate optical imaging.

3 . The system of claim 1 , the system of capable of achieving optical imaging on multiple simultaneous data acquisition channels.

Assignees

• Purdue Research Foundation

Inventors

• Simpson Garth Jason
• Bouman Charles Addison
• Muir Ryan Douglas
• Sullivan Shane
• Newman Justin Allen
• Carlsen Mark

Classifications

• G02B21/0084 Primary

  time-scale detection, e.g. strobed, ultra-fast, heterodyne detection · CPC title

• G02B21/0048

  scanning mirrors, e.g. rotating or galvanomirrors, MEMS mirrors · CPC title

• G02B21/0076

  arrangements using fluorescence or luminescence · CPC title

• G02B26/101

  with both horizontal and vertical deflecting means, e.g. raster or XY scanners (colour television using laser beams scanning a display screen H04N9/3129) · CPC title

• G02B21/008

  Details of detection or image processing, including general computer control · CPC title