Strain for producing succinate from carbon dioxide and method for succinate production using the strain

Patent metadata

Field	Value
Publication number	US-2016002677-A1
Application number	US-201414494930-A
Country	US
Kind code	A1
Filing date	Sep 24, 2014
Priority date	Jul 2, 2014
Publication date	Jan 7, 2016
Grant date	—

Abstract

The present disclosure relates to a strain capable of producing succinate using starch accumulated in microalgae which grow using carbon dioxide as a direct carbon source without converting it to glucose and a method for producing succinate using the same. The present disclosure provides a strain producing succinate from carbon dioxide, selected from a group consisting of Corynebacterium glutamicum BL-1-pBlAmyS (KCTC 12585BP) and Corynebacterium glutamicum BL-1-pSbAmyA (KCTC 12587BP). The present disclosure also provides a method for producing succinate from carbon dioxide, including fermenting starch by inoculating the strain producing succinate from carbon dioxide in a starch-containing medium.

First claim

What is claimed is:

1 . A strain producing succinate from carbon dioxide, selected from a group consisting of Corynebacterium glutamicum BL-1-pBlAmyS (KCTC 12585BP) and Corynebacterium glutamicum BL-1-pSbAmyA (KCTC 12587BP).

2 . The strain producing succinate from carbon dioxide according to claim 1 , wherein the strain producing succinate from carbon dioxide grows using starch accumulated in microalgal biomass that grows using carbon dioxide as a direct carbon source

3 . The strain producing succinate from carbon dioxide according to claim 1 , wherein the growth rate of strain producing succinate from carbon dioxide is 3 times or more higher in a starch-containing medium as compared to the mother strain Corynebacterium glutamicum BL-1 not including the gene AmyA or AmyS.

4 . A method for preparing the strain producing succinate from carbon dioxide according to claim 1 , comprising:
preparing torA-SbAmyA (SEQ ID NO 1) by attaching TorA signal peptide to the coding sequence of AmyA or preparing torA-BlAmyS (SEQ ID NO 2) by attaching TorA signal peptide to the coding sequence of AmyS; inserting the torA-SbAmyA (SEQ ID NO 1) or the torA-BlAmyS (SEQ ID NO 2) to a vector expressible in Corynebacterium glutamicum BL-1 as a mother strain and genetically recombining the same with the mother strain Corynebacterium glutamicum BL-1; and overexpressing the inserted torA-SbAmyA (SEQ ID NO 1) or torA-BlAmyS (SEQ ID NO 2) in the mother strain.

5 . A method for producing succinate from carbon dioxide, comprising fermenting starch by inoculating the strain producing succinate from carbon dioxide according to claim 1 in a starch-containing medium.

6 . The method for producing succinate from carbon dioxide according to claim 5 , wherein the starch-containing medium is a medium containing a microalgal biomass lysate in which carbon dioxide is fixed.

Assignees

• Korea Inst Sci & Tech

Inventors

• Woo Han Min
• Lee Jung Seok
• Um Youngsoon
• Gong Gyeong Taek
• Min Byoung Koun
• Ha Jeong-Myeong
• Kim Yunje
• Sim Sang Jun

Classifications

• C12N15/77

  for Corynebacterium; for Brevibacterium · CPC title

• C12P7/46 Primary

  Dicarboxylic acids having four or less carbon atoms, e.g. fumaric acid, maleic acid · CPC title