Placental tissue grafts and improved methods of preparing and using the same

Patent metadata

Field	Value
Publication number	US-2016000968-A1
Application number	US-201414325132-A
Country	US
Kind code	A1
Filing date	Jul 7, 2014
Priority date	Jul 7, 2014
Publication date	Jan 7, 2016
Grant date	—

Abstract

Described herein are tissue grafts derived from the placenta. The grafts are composed of at least one layer of amnion which has been decellularized and at least one layer of chorion. Also described herein are methods for making and using the tissue grafts.

First claim

1 . A laminated tissue graft comprising:
a first membrane comprising modified decellularized amnion, wherein the modified amnion comprises an exposed basement membrane; a second membrane comprising cleaned and washed chorion membrane retaining a cellular component layered adjacent to the first membrane, said chorion membrane layered adjacent to said first membrane; and optionally one or more additional membranes layered over the first or second membrane, wherein the one or more additional membranes is selected from the group consisting of amnion, decellularized amnion, chorion, decellularized chorion, allograft pericardium, allograft acellular dermis, amniotic membrane, Wharton's jelly, and combinations thereof; wherein the laminated tissue graft is dehydrated.

2 . The laminated tissue graft of claim 1 , wherein one side of the first membrane is a decellularized fibroblast layer.

3 . The laminated tissue graft of claim 1 , wherein one side of the first membrane is an exposed compact layer.

4 . The laminated tissue graft of claim 1 , wherein one side of the first membrane is the other side of the basement membrane, wherein the other side of the basement membrane is exposed.

5 . (canceled)

6 . The laminated tissue graft of claim 1 , further comprising at least one component selected from the group consisting of a naturally occurring growth factor, a platelet concentrate, a bone marrow aspirate, stem cells, or an antibiotic.

Assignees

• Mimedx Group Inc

Inventors

• Koob Thomas J
• Brown Rebeccah J C
• Dean Deborah L
• Petit Parker

Classifications

• A61L27/3683 Primary

  subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment · CPC title

• A61L27/3604 Primary

  characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel · CPC title

• A61L27/3695

  characterised by the function or physical properties of the final product, where no specific conditions are defined to achieve this (A61L27/3687, A61L27/3691 take precedence) · CPC title

• A61L27/54

  Biologically active materials, e.g. therapeutic substances {(A61L27/227 takes precedence)} · CPC title

• A61L2430/16

  for reconstruction of eye parts, e.g. intraocular lens, cornea · CPC title