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Mirna profiling compositions and methods of use
US2016000940A1 · US · A1
| Field | Value |
|---|---|
| Publication number | US-2016000940-A1 |
| Application number | US-201414769398-A |
| Country | US |
| Kind code | A1 |
| Filing date | Feb 26, 2014 |
| Priority date | Feb 28, 2013 |
| Publication date | Jan 7, 2016 |
| Grant date | — |
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Abstract
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Disclosed herein is a nanosensor of miRNA activity in a target cell, and methods of use, for detection and diagnostic applications. The nanosensor comprises a delivery particle comprising an iron oxide crystal coated with a polymer; and a sensor oligonucleotide covalently attached to the polymer. The sensor oligonucleotide comprises a seed region, comprising a nucleic acid sequence that is completely complementary to the target miRNA and comprises a cleavage site which can be engaged and cleaved by the target miRNA.
First claim
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1 . A nanosensor for detection of miRNA activity in a target cell comprising: a) a delivery particle comprising an iron oxide crystal coated with a polymer; and b) a sensor oligonucleotide covalently attached to the polymer, comprising: i) a seed region comprising a nucleic acid sequence that is completely complementary to the target miRNA and comprises a cleavage site which can be engaged and cleaved by the target miRNA; ii) two non-seed regions that each flank the seed region and are each comprised of a nucleic acid sequence that is complementary to the target miRNA to promote hybridization of the sensor oligonucleotide to the target miRNA; and iii) members of a quencher-fluorophore pair; wherein the quencher fluorophore pair members respectively flank the cleavage site and are separated by a distance that permits significant quenching of emitted fluorescent signal. 2 .- 71 . (canceled)
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- A61K49/10Primary
Organic compounds · CPC title
Nucleic acid detection involving sensors · CPC title
for cancer (immunoassay for cancer G01N33/575) · CPC title
involving a quantitation step · CPC title
miRNA, siRNA or ncRNA · CPC title
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- What does patent US2016000940A1 cover?
- Disclosed herein is a nanosensor of miRNA activity in a target cell, and methods of use, for detection and diagnostic applications. The nanosensor comprises a delivery particle comprising an iron oxide crystal coated with a polymer; and a sensor oligonucleotide covalently attached to the polymer. The sensor oligonucleotide comprises a seed region, comprising a nucleic acid sequence that is comp…
- Who is the assignee on this patent?
- Gen Hospital Corp
- What technology area does this patent fall under?
- Primary CPC classification A61K49/10. Mapped technology areas include Human Necessities.
- When was this patent published?
- Publication date Thu Jan 07 2016 00:00:00 GMT+0000 (Coordinated Universal Time) (A1). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).