Quantitative pcr method using internal control
US-2024368681-A1 · Nov 7, 2024 · US
Pcr primers
US2015225775A1 · US · A1
| Field | Value |
|---|---|
| Publication number | US-2015225775-A1 |
| Application number | US-201514614680-A |
| Country | US |
| Kind code | A1 |
| Filing date | Feb 5, 2015 |
| Priority date | Feb 7, 2014 |
| Publication date | Aug 13, 2015 |
| Grant date | — |
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Abstract
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The present disclosure provides methods, compositions, and kits for performing PCR (including multiplex PCR). The methods, compositions and kits provided herein use one or more primer pairs that contain one or more cleavable bases located at a minimal distance away from the 3′ termini of the primers, and increase the accuracy of downstream analysis of sequence data.
First claim
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1 . A method for performing polymerase chain reaction (PCR), comprising: a) amplifying one or more different target nucleic acids in the presence of one or more different primer pairs specific to the one or more different target nucleic acids in a single reaction mixture via PCR, wherein each primer of the one or more different primer pairs contains one or more cleavable bases, and wherein in substantially all of the primers of the one or more different primer pairs, each of the one or more c…
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- C12Q1/686Primary
Chemistry & Metallurgy · mapped topic
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- What does patent US2015225775A1 cover?
- The present disclosure provides methods, compositions, and kits for performing PCR (including multiplex PCR). The methods, compositions and kits provided herein use one or more primer pairs that contain one or more cleavable bases located at a minimal distance away from the 3′ termini of the primers, and increase the accuracy of downstream analysis of sequence data.
- Who is the assignee on this patent?
- Qiagen Sciences Llc
- What technology area does this patent fall under?
- Primary CPC classification C12Q1/686. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Thu Aug 13 2015 00:00:00 GMT+0000 (Coordinated Universal Time) (A1). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).