Methods for Nucleic Acid Cleavage
US-2024417778-A1 · Dec 19, 2024 · US
Phenotype-neutral barcodes for digital analysis
US2015225770A1 · US · A1
| Field | Value |
|---|---|
| Publication number | US-2015225770-A1 |
| Application number | US-201414178665-A |
| Country | US |
| Kind code | A1 |
| Filing date | Feb 12, 2014 |
| Priority date | Feb 12, 2014 |
| Publication date | Aug 13, 2015 |
| Grant date | — |
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Abstract
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Processes of specifically and effectively labeling an organism are provided. Processes involve the incorporation of a plurality of phenotype neutral tags that are differentially detected where the presence or absence of the tag is represented by a digital readout. The incorporation of stealth tags or insertion tags provides a rapid and population maintaining labeling of an organism that can be readily identified by digital PCR techniques.
First claim
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1 . A process of labeling an organism comprising: selecting a labeling region within an exon of a gene; substituting a plurality of non-wild type nucleotides within said labeling region to produce a plurality of tags within said labeling region, said plurality of tags representing a barcode, at least two of said plurality of tags each in a separate codon resulting in synonymous substitutions at each nucleotide substitution site; said step of substituting producing silent mutations in said gen…
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Chemistry & Metallurgy · mapped topic
- C12Q1/6806Primary
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
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- What does patent US2015225770A1 cover?
- Processes of specifically and effectively labeling an organism are provided. Processes involve the incorporation of a plurality of phenotype neutral tags that are differentially detected where the presence or absence of the tag is represented by a digital readout. The incorporation of stealth tags or insertion tags provides a rapid and population maintaining labeling of an organism that can be …
- Who is the assignee on this patent?
- Us Army Res Lab Attn Rdrl Loc I
- What technology area does this patent fall under?
- Primary CPC classification C12Q1/6806. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Thu Aug 13 2015 00:00:00 GMT+0000 (Coordinated Universal Time) (A1). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).