Lipase variants and compositions comprising surfactant and lipase variant

Patent metadata

Field	Value
Publication number	US-12595442-B2
Application number	US-202318392370-A
Country	US
Kind code	B2
Filing date	Dec 21, 2023
Priority date	Jun 30, 2016
Publication date	Apr 7, 2026
Grant date	Apr 7, 2026

Abstract

The present invention relates to lipase variants and compositions comprising (i) at least one surfactant and (ii) at least one lipase variant of the invention. Furthermore, the present invention relates to methods of using the compositions.

First claim

The invention claimed is:

1 . A lipase variant of a parent lipase, which (a) comprises a substitution at positions corresponding to positions 1, 210, and 233 of SEQ ID NO: 1; (b) has a sequence identity of at least 85% but less than 100% sequence identity to SEQ ID NO: 1; and (c) has lipase activity.

2 . The lipase variant according to claim 1 , wherein the variant further comprises a modification in at least one of the following positions: V2, D27, N33, G38, F51, E56, L69, D96, K98, D111, G163, V176, H198, Y220, L227, T231, K237, D254, and P256 of SEQ ID NO: 1.

3 . The lipase variant according to claim 1 , wherein the variant further comprises at least one of the following substitutions: V2Y, D27R, N33K, N33Q, G38A, F51V, E56K, L69R, D96E, D96L, K98I, K98Q, D111A, G163K, V176L, H198S, Y220F, L227G, T231R, N233C, K237C, D254S, and P256T of SEQ ID NO: 1.

4 . The lipase variant of claim 1 , comprising E210T/N/A/Q/R.

5 . The lipase variant of claim 1 , comprising N233C or N233R.

6 . The lipase variant of claim 1 , comprising E1C.

7 . The lipase variant of claim 1 , further comprising a modification in at least one of the following positions: V2, N33, G38, E56, L69, V176, L227, K237, and D254 of SEQ ID NO: 1.

8 . The lipase variant of claim 1 , further comprising a modification selected from the following substitutions: V2Y, N33K, N33Q, G38A, E56K, L69R, V176L, L227G, K237C, and D254S.

9 . The lipase variant of claim 1 , which has a sequence identity of at least 90% sequence identity to SEQ ID NO: 1.

10 . The lipase variant of claim 1 , which has a sequence identity of at least 95% sequence identity to SEQ ID NO: 1.

11 . The lipase variant of claim 1 , wherein the parent lipase is SEQ ID NO: 1.

12 . A composition comprising a lipase variant of claim 1 and a surfactant.

13 . The composition of claim 12 , further comprising at least one additional enzyme selected from the group consisting of amylase, cellulase, beta-glucanase, mannanase, and protease.

14 . A method of laundering, comprising laundering a fabric with a composition according to claim 12 at a temperature of 50° C. or less.

15 . A method of pre-treating a fabric with a composition according to claim 12 , comprising the steps of adding said composition to said fabric, and leaving the composition on the fabric for a period of time, and rinsing off said composition from said fabric.

Assignees

• Novozymes As

Inventors

• Borch Kim
• Hansen Carsten Hørslev
• Vind Jesper
• Malten Marco
• Kaasgaard Svend Gunnar

Classifications

• C11D2111/12

  Soft surfaces, e.g. textile · CPC title

• C12N9/20 Primary

  Triglyceride splitting, e.g. by means of lipase · CPC title

• C11D3/50

  Perfumes · CPC title

• C11D3/38618

  Protease or amylase in liquid compositions only · CPC title

• C11D3/38609

  Protease or amylase in solid compositions only · CPC title