Fucose-binding protein, method for producing same, and use of same

Patent metadata

Field	Value
Publication number	US-12595286-B2
Application number	US-201917267891-A
Country	US
Kind code	B2
Filing date	Aug 8, 2019
Priority date	Aug 13, 2018
Publication date	Apr 7, 2026
Grant date	Apr 7, 2026

Abstract

The present invention aims to provide a fucose-binding protein that shows improved productivity in cases of expression in a host such as Escherichia coli , improved binding affinity to a fucose-containing sugar chain such as a sugar chain containing a structure composed of Fucα1-2Galβ1-3GlcNAc and/or Fucα1-2Galβ1-3GalNAc, and/or improved thermal stability. The above object is achieved by deleting a plurality of amino acid residues in the C-terminal side of the amino acid sequence of the fucose-binding protein BC2LCN of SEQ ID NO: 1, and, when necessary, substituting the glycine residue at position 36 in SEQ ID NO: 1 with a cysteine residue, substituting the glutamine residue at position 39 in SEQ ID NO: 1 with a leucine residue or methionine residue, substituting the glutamine residue at position 65 in SEQ ID NO: 1 with a leucine residue, substituting the cysteine residue at position 72 in SEQ ID NO: 1 with a glycine residue or alanine residue, substituting the glutamic acid residue at position 81 in SEQ ID NO: 1 with a cysteine residue, glutamine residue, histidine residue, or methionine residue, and/or substituting the glycine residue identified as the residue at position 36 in SEQ ID NO: 1 with a cysteine residue.

First claim

The invention claimed is:

1 . A fucose-binding protein comprising an amino acid sequence having at least 90% identity to amino acid residues 1 to X of SEQ ID NO: 1, wherein X is an integer of 110-140, wherein the amino acid sequence comprises at least one amino acid substitution at an amino acid residue position corresponding to an amino acid residue position of SEQ ID NO: 1 selected from the group consisting of residue position 39, 72, 65, 81, and 36;
wherein the fucose-binding…

Assignees

• Tosoh Corp
• Sagami Chemical Res Institute

Inventors

• Hayashi Masahiro
• Maruyama Takahiro
• Ito Hiroyuki
• Asagoshi Aya
• Hatayama Kouta
• Hoya Megumi

Classifications

• C12N5/0693

  Chemistry & Metallurgy · mapped topic

• C07H1/06

  Chemistry & Metallurgy · mapped topic

• B01J20/30

  Operations & Transport · mapped topic

• B01J20/24

  Operations & Transport · mapped topic

• B01D15/3823 Primary

  Operations & Transport · mapped topic