Method and apparatus for determining ketosis

Patent metadata

Field	Value
Publication number	US-12590944-B2
Application number	US-202118015792-A
Country	US
Kind code	B2
Filing date	Jul 12, 2021
Priority date	Jul 17, 2020
Publication date	Mar 31, 2026
Grant date	Mar 31, 2026

Abstract

An object of the present invention is to make it possible to easily determine a presence or absence of ketosis and/or the possibility of ketosis in an animal on site or the like. Another object of the present invention is to provide an apparatus for performing the determination. A method for determining ketosis according to one embodiment of the present invention includes providing gas detection means with a gas generated from a body fluid collected from an animal (except for humans), and determining ketosis of the animal based on a composition of the gas generated from the body fluid using a response of the gas detection means to the gas generated from the body fluid.

First claim

The invention claimed is:

1 . A method for determining ketosis, comprising:
providing gas detection means with a gas generated from a body fluid collected from an animal other than humans; and determining ketosis of the animal based on a composition of the gas generated from the body fluid using a response of the gas detection means to the gas generated from the body fluid, wherein the gas detection means is a gas sensor, the gas sensor being a surface stress sensor, the response of the gas detection means is a signal output from the surface stress sensor, and the determining of ketosis is performed based on a pattern of a temporal change in the signal output from the surface stress sensor, wherein the gas generated from the body fluid and a purge gas are alternately supplied to the surface stress sensor, and the determining of ketosis is performed using the signal output from the surface stress sensor corresponding to the gas generated from the body fluid and the signal output from the surface stress sensor corresponding to the purge gas, wherein an offset treatment is performed to the signal output from the surface stress sensor, a reference point of the offset treatment is set at an end point of a time frame during which the purge gas is given to the surface stress sensor, and a determination point is set at an arbitrary time point when a certain time has elapsed from an end of a time frame during which the gas generated from the body fluid is supplied to the surface stress sensor, and wherein the body fluid is selected from the group consisting of milk, blood, urine, saliva, and sweat.

2 . The method for determining ketosis according to claim 1 , wherein the gas generated from the body fluid is at least one of ketones, alcohols, aldehydes, nitriles, organic acids, water vapor, nitrogen, oxygen, and carbon dioxide.

3 . The method for determining ketosis according to claim 2 , wherein the gas generated from the body fluid is at least one of ketones and alcohols, the ketones being acetone, and the alcohols being at least one selected from the group consisting of methanol, ethanol, propanol, and butanol.

4 . The method for determining ketosis according to claim 1 , wherein, as the gas generated from the body fluid, a gas obtained by passing a gas substantially not containing a component that affects the determination of ketosis through a container containing the body fluid is supplied to the surface stress sensor.

5 . The method for determining ketosis according to claim 1 , wherein the surface stress sensor is a membrane type surface stress sensor.

6 . The method for determining ketosis according to claim 1 , wherein at least one selected from the group consisting of poly(4-methylstyrene), poly(2,6-diphenyl-p-phenylene oxide), poly(vinylidene fluoride), cellulose acetate butyrate, poly(ethyleneimine), phenyl group-modified silica/titania composite nanoparticles, and polystyrene is used as a material of a sensitive film of the surface stress sensor.

7 . The method for determining ketosis according to claim 6 , wherein, as the surface stress sensor, at least a first surface stress sensor using one material selected from the group as the sensitive film and a second surface stress sensor using another material selected from the group as the sensitive film are used.

8 . The method for determining ketosis according to claim 1 , wherein a time frame during which a predetermined reference gas is given to the surface stress sensor is provided in addition to the time frame during which the gas generated from the body fluid is supplied to the surface stress sensor and the time frame during which the purge gas is given to the surface stress sensor, and the signal output from the surface stress sensor corresponding to the reference gas is further used in the determination of the ketosis.

9 . The method for determining ketosis according to claim 8 , wherein the predetermined reference gas is a gas generated from a reference body fluid.

10 . The method for determining ketosis according to claim 1 , wherein
a reference gas and the purge gas are alternately supplied to the surface stress sensor to prepare digital data corresponding to the reference gas, the digital data corresponding to the reference gas being based on the signal output from the surface stress sensor corresponding to the reference gas and the signal output from the surface stress sensor corresponding to the purge gas, the gas generated from the body fluid and the purge gas are alternately supplied to the surface stress sensor to prepare digital data corresponding to the body fluid to be measured, the digital data corresponding to the body fluid to be measured being based on the signal output from the surface stress sensor corresponding to the gas generated from the body fluid and the signal output from the surface stress sensor corresponding to the purge gas, and the determination of ketosis is performed based on the digital data corresponding to the reference gas and the digital data corresponding to the body fluid to be measured.

11 . The method for determining ketosis according to claim 10 , wherein the surface stress sensor to which the reference gas and the purge gas are alternately supplied and the surface stress sensor to which the gas generated from the body fluid and the purge gas are alternately supplied are the same surface stress sensor, or are surface stress sensors that are different from each other.

12 . The method for determining ketosis according to claim 1 , wherein the gas generated from the body fluid is supplied to an additional gas sensor, and the determination of ketosis is performed based on the signal output from the surface stress sensor and a signal output from the additional gas sensor.

13 . An apparatus used for the method for determining ketosis according to claim 1 , comprising:
at least one surface stress sensor; a first gas flow path for supplying a sample gas generated from a body fluid to be examined collected from an animal, the body fluid being selected from the group consisting of milk, blood, urine, saliva, and sweat; and a second gas flow path for supplying a purge gas not containing a gas component to be measured, wherein the sample gas supplied from the first gas flow path and the purge gas supplied from the second gas flow path are alternately switched and supplied to the at least one surface stress sensor to generate a signal from the at least one surface stress sensor, thereby determining ketosis of the animal based on a composition of the gas generated from the body fluid, and wherein the apparatus performs an offset treatment to the signal output from the at least one surface stress sensor, wherein a reference point of the offset treatment is set at an end point of a time frame during which the purge gas is given to the at least one surface stress sensor, and a determination point is set at an arbitrary time point when a certain time has elapsed from an end of a time frame during which the sample gas is supplied to the at least one surface stress sensor.

14 . The apparatus according to claim 13 , further comprising
an additional gas sensor and an additional gas flow path for supplying the sample gas to the additional gas sensor, wherein the determination of ketosis is performed based on the signal output from the at least one surface stress sensor and a signal output from the additional gas sensor.

15 . The method for determining ketosis according to claim 1 , wherein the presence or absence of ketosis or a possibility of ketosis is determined by obtaining a feature value from the det

Assignees

• Nat Inst Materials Science

Inventors

• NAKAKUBO Ryoh
• Ishida Mitsuyoshi
• Yoshikawa Genki
• Minami Kosuke
• IMAMURA Gaku
• Matsuzaka Hideki
• Nemoto Takahiro

Classifications

• G01N2800/04

  Endocrine or metabolic disorders · CPC title

• G01N33/98

  involving alcohol, e.g. ethanol in breath · CPC title

• G01N33/64

  involving ketones · CPC title

• G01N33/487 Primary

  of liquid biological material · CPC title

• G01N33/493 Primary

  urine · CPC title