Impeller and sparger assemblies for a bioprocessing system

Patent metadata

Field	Value
Publication number	US-12589366-B2
Application number	US-201917296662-A
Country	US
Kind code	B2
Filing date	Dec 4, 2019
Priority date	Dec 14, 2018
Publication date	Mar 31, 2026
Grant date	Mar 31, 2026

Abstract

A sparger assembly ( 700 ) for a bioprocessing system includes a base plate ( 710 ) and at least one aeration manifold ( 712, 714 ) removably connected to tire base plate. Each aeration manifold includes at least one inlet for receiving a gas and a plurality of gas outlet openings for delivering tire gas to a fluid within the bioprocessing system. An impeller assembly ( 740 ) fora bioprocessing system includes a hub and at least one blade ( 742 ) operatively connected to the hub. The at least one blade includes a first portion connected to the hub and extending generally vertically, and a second portion extending at an upward angle from tire first portion.

First claim

The invention claimed is:

1 . A sparger assembly for a bioprocessing system, comprising:
a base plate; and at least one aeration manifold removably connected to the base plate, each aeration manifold including at least one inlet for receiving a gas and a plurality of gas outlet openings for delivering the gas to a fluid within the bioprocessing system; and an impeller assembly comprising a hub and at least one blade operatively connected to the hub, the at least one blade including a first portion connected to the hub and extending generally vertically, a second portion extending at an upward angle from the first portion, and a third portion extending from the second portion, wherein the third portion is substantially horizontal, wherein the third portion has a distal end and a proximal end, with the distal end being wider than the proximal end, wherein the base plate includes a mounting device enabling coupling of the impeller assembly to the base plate adjacent to the at least one aeration manifold; and wherein the at least one aeration manifold is arcuate in shape.

2 . The sparger assembly of claim 1 , wherein the at least one aeration manifold is in spaced vertical relation to the base plate when connected to the base plate.

3 . The sparger assembly of claim 1 , wherein the at least one aeration manifold is annular in shape.

4 . The sparger assembly of claim 1 , wherein said at least one aeration manifold comprises:
an inlet chamber unit with an inlet chamber fluidically connected to a tube connector; a porous plate sealed to said inlet chamber unit by a gasket; and a frame fastened to said inlet chamber unit and engaging said porous plate to keep the plate and the gasket in sealing abutment with the inlet chamber unit.

5 . The sparger assembly of claim 4 , wherein said frame is fastened to said inlet chamber unit by a plurality of snap joints.

6 . The sparger assembly of claim 4 , wherein said inlet chamber unit comprises a plurality of feet, fastened to posts in said base plate by snap joints.

7 . The sparger assembly of claim 1 , wherein the at least one aeration manifold is two aeration manifolds including a first aeration manifold and a second aeration manifold, the aeration manifolds being arranged in a circular configuration on the base plate.

8 . The sparger assembly of claim 1 , wherein the at least one aeration manifold is four aeration manifolds, the aeration manifolds being arranged in a circular configuration on the base plate.

9 . The sparger assembly of claim 8 , wherein a size of the gas outlet openings of at least one of the aeration manifolds is different from a size of the gas outlet openings of at least another of the aeration manifolds.

10 . The sparger assembly of claim 7 , wherein the aeration manifolds are arranged on the base plate such that the size of the gas outlet openings of each aeration manifold is different from the size of the gas outlet openings of an immediately adjacent aeration manifold.

11 . The sparger assembly of claim 8 , wherein:
a first pair of the aeration manifolds are arranged on the base plate to form a first semi-circle; and a second pair of the aeration manifolds are arranged on the base plate to form a second semi-circle; wherein the gas outlet openings of the first pair of aeration manifolds are a first size; and wherein the gas outlet openings of the second pair of aeration manifolds are a second size that is different from the first size.

12 . The sparger assembly of claim 1 , wherein:
the at least one aeration manifold is at least two aeration manifolds including a first aeration manifold and a second aeration manifold concentrically mounted with the first aeration manifold; wherein at least one of the first aeration manifold and the second aeration manifold has a pleated periphery.

13 . The sparger assembly of claim 1 , wherein:
the at least one aeration manifold is a plurality of aeration manifolds; and wherein at least one of the aeration manifolds is raised above the base plate a first distance; wherein at least another of the aeration manifolds is raised above the base plate at a second distance; and wherein the first distance is greater than the second distance.

14 . The sparger assembly of claim 1 , wherein the base plate includes a shaft for receiving the impeller assembly and positioning the impeller assembly so that blades of the impeller assembly are located a distance above the at least one aeration manifold.

15 . The sparger assembly of claim 1 , wherein the base plate includes an aperture for interfacing with a drain port in a flexible bioprocessing bag and a drain port of a support vessel that receives the flexible bioprocessing bag.

16 . The sparger assembly of claim 1 , wherein the third portion is substantially triangular in shape.

17 . The sparger assembly of claim 4 , wherein the frame comprises one or more ribs extending between opposing sides of the fame.

Assignees

• Global Life Sciences Solutions Usa Llc

Inventors

• Damren Richard
• Becker Timothy
• Tuohey Colin
• Stankowski Ralph
• Bandaru Sree Ramulu
• Balakrishnan Saravanan
• Falkman Thomas
• Le Greves Pierre
• Dalmo Tomas
• Rao Nagaraj
• Petersson Mikael
• Dash Manas

Classifications

• C12M29/06 Primary

  Nozzles; Sprayers; Spargers; Diffusers (per se B01F23/231, B01J19/26) · CPC title

• C12M27/02

  Stirrer or mobile mixing elements · CPC title

• C12M23/26

  flexible (flexible containers for laboratory use B01L3/505) · CPC title

• C12M23/14

  Bags · CPC title

• B01F2101/44

  Mixing of ingredients for microbiology, enzymology, in vitro culture or genetic manipulation · CPC title