Acoustic separation of a test sample

Patent metadata

Field	Value
Publication number	US-12584902-B2
Application number	US-202318534652-A
Country	US
Kind code	B2
Filing date	Dec 10, 2023
Priority date	Dec 10, 2023
Publication date	Mar 24, 2026
Grant date	Mar 24, 2026

Abstract

An example system includes a detector configured to detect a value that is based on a hematocrit of a test sample containing plasma and red blood cells, a fluidic channel configured to hold the test sample, and an acoustic transducer configured to apply acoustic waves to the fluidic channel to separate the plasma from the red blood cells in the fluidic channel. The acoustic waves have at least one of an amplitude, a frequency, or a duration that is based on the value.

First claim

What is claimed is:

1 . A system comprising:
a detector configured to detect a value that is based on a hematocrit of a test sample comprised of plasma and red blood cells; a fluidic channel configured to hold the test sample; an acoustic transducer configured to apply acoustic waves to the fluidic channel to separate the plasma from the red blood cells in the fluidic channel, the acoustic waves having at least one of an amplitude, a frequency, or a duration that is based on the value; memory storing a look-up table (LUT), the LUT storing:
multiple voltages, each voltage corresponding to an amplitude of acoustic waves for a specified value;
multiple frequencies, each frequency corresponding to a frequency of acoustic waves for a specified value; and
multiple durations, each duration corresponding to a duration of acoustic waves to be applied for a specified value; and
a control system configured to control the acoustic transducer based on the value, wherein controlling the acoustic transducer based on value comprises:
selecting a voltage from the LUT based on the value;
selecting a frequency from the LUT based on the value;
selecting a duration from the LUT based on the value; and
controlling the acoustic transducer based on the voltage selected, the frequency selected, and the duration selected.

2 . The system of claim 1 , further comprising:
an optical detector configured to perform optical detection on the plasma separated in the fluidic channel; wherein the control system is configured to determine hemolysis in the test sample based on a result of the optical detection.

3 . The system of claim 1 , wherein the value comprises fluid electrical resistance; and
wherein all of the amplitude, the frequency, and the duration of the acoustic waves are based on the fluid electrical resistance.

4 . The system of claim 1 ,
wherein the control system is configured to apply the voltage selected to the acoustic transducer to produce acoustic waves having the amplitude.

5 . The system of claim 1 , wherein the value comprises fluid electrical resistance that corresponds to a voltage difference between two locations in the test sample, the fluid electrical resistance varying with the hematocrit.

6 . The system of claim 1 , wherein separating the plasma from the red blood cells in the fluidic channel comprises forming a region of plasma in a center of the fluidic channel.

7 . The system of claim 1 , wherein separating the plasma from the red blood cells in the fluidic channel comprises forming a region of red blood cells in a center of the fluidic channel.

8 . The system of claim 1 ,
wherein the detector is configured to detect changes over time in the value; and wherein the control system is configured to control the acoustic transducer to adjust at least one of the amplitude, the frequency, or the duration of the acoustic waves applied to the fluidic channel based on a detected change to the value over time.

9 . The system of claim 1 ,
wherein controlling the acoustic transducer based on the value comprises:
selecting a drive routine from among multiple drive routines stored in memory, the drive routine specifying, for a corresponding value based on the hematocrit, a voltage corresponding to an amplitude of acoustic waves to be produced by the acoustic transducer, a frequency of acoustic waves to be produced by the acoustic transducer, and the duration of acoustic waves to be applied by the acoustic transducer; and
wherein controlling the acoustic transducer based on the voltage selected, the frequency selected, and the duration selected comprises controlling the acoustic transducer in accordance with the drive routine that was selected.

10 . The method of claim 1 , wherein separating the plasma from the red blood cells in the fluidic channel comprises forming a region of plasma in a center of the fluidic channel; or
wherein separating the plasma from the red blood cells in the fluidic channel comprises forming a region of red blood cells in a center of the fluidic channel.

11 . A method comprising:
determining a value based on a hematocrit of a test sample comprised of plasma and red blood cells; applying acoustic waves to a fluidic channel containing the test sample to separate the plasma from the red blood cells, at least one of an amplitude, a frequency, or a duration of the acoustic waves applied to the fluidic channel being based on the value; performing optical detection on the plasma; determining hemolysis of the test sample based on a result of the optical detection; wherein memory stores a look-up table (LUT), the LUT storing:
multiple voltages, each voltage corresponding to an amplitude of acoustic waves for a specified value;
multiple frequencies, each frequency corresponding to a frequency of acoustic waves for a specified value; and
multiple durations, each duration corresponding to a duration of acoustic waves to be applied for a specified value; and
wherein applying the acoustic waves is controlled by a control system, the control system controlling applying the acoustic waves by performing operations comprising:
selecting a voltage from the LUT based on the value;
selecting a frequency from the LUT based on the value;
selecting a duration from the LUT based on the value; and
controlling applying the acoustic waves based on the voltage selected, the frequency selected, and the duration selected.

12 . The method of claim 11 , wherein the amplitude, the frequency, and the duration of the acoustic waves applied to the fluidic channel are all based on the value.

13 . The method of claim 11 , wherein the acoustic waves are applied by an acoustic transducer; and
wherein controlling the amplitude comprises applying the voltage selected to the acoustic transducer.

14 . The method of claim 11 , wherein determining the value comprises:
measuring a voltage difference between two locations in the test sample, the voltage difference corresponding to a fluid electrical resistance of the test sample, the fluid electrical resistance varying with the hematocrit.

15 . The method of claim 11 , wherein detecting the value of the test sample is performed over time; and
wherein the method comprises changing at least one of the amplitude, the frequency, or the duration of the acoustic waves applied to the fluidic channel based on a detected change to the value over time.

16 . A system comprising:
a detector configured to detect a value that is based on a hematocrit of a test sample comprised of plasma and red blood cells; a fluidic channel configured to hold the test sample; an acoustic transducer configured to apply acoustic waves to the fluidic channel to separate the plasma from the red blood cells in the fluidic channel; a control system configured to control the acoustic transducer to produce acoustic waves that sweep over a range of frequencies, the range of frequencies being based on the value; and memory storing a look-up table (LUT), the LUT storing:
multiple voltages, each voltage corresponding to an amplitude of acoustic waves for a specified value;
multiple ranges of frequencies, each range of frequency corresponding to a range of frequency of acoustic waves for a specified value; and
multiple durations, each duration corresponding to a duration of acoustic waves to be applied for a specified value;
wherein the control system is configured to control the acoustic transducer by performing operations comprising:
selecting a voltage from the LUT based on the value;
selecting a range of frequenci

Assignees

• Instr Laboratory Co

Inventors

• Balasubramanian Shankar
• Phan Nam
• Adib Lara
• Wang Yu
• Vanderslice Nicholas

Classifications

• G01N29/34

  Generating the ultrasonic, sonic or infrasonic waves {, e.g. electronic circuits specially adapted therefor} · CPC title

• G01N15/1459

  the analysis being performed on a sample stream · CPC title

• B01L2300/0645

  Electrodes · CPC title

• B01L2400/0436

  acoustic forces, e.g. surface acoustic waves [SAW] · CPC title

• B01L2200/0652

  Sorting or classification of particles or molecules · CPC title